{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T09:53:08Z","timestamp":1695635588846},"reference-count":10,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1966,10,1]],"date-time":"1966-10-01T00:00:00Z","timestamp":-102643200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1966,10]]},"abstract":"<jats:p><jats:sc>Adamson, David<\/jats:sc>M. (University of Oklahoma, Norman),<jats:sc>and George C. Cozad<\/jats:sc>. Immunoelectrophoretic studies of sera from rabbits experimentally infected with<jats:italic>Histoplasma capsulatum<\/jats:italic>. J. Bacteriol.<jats:bold>92:<\/jats:bold>887\u2013891. 1966.\u2014In a study involving 24 mature Dutch rabbits inoculated intravenously with 2.6 \u00d7 10<jats:sup>8<\/jats:sup><jats:italic>Histoplasma capsulatum<\/jats:italic>yeast cells per kg of body weight, all rabbits were shown to develop precipitins by the end of the 5th week. Precipitins were demonstrated both by the micro-Ouchterlony method and microimmunoelectrophoresis with the use of concentrated histoplasmin as the antigen. The latter technique proved to be the more sensitive. The 10 \u00d7 concentrate of histoplasmin gave better precipitin patterns than did the 5 \u00d7 concentrate. Agglutinins were formed by 90% of the rabbits by the end of the 4th week, as shown by the capillary tube agglutination test. Three precipitin bands, arbitrarily designated A, B, and C, were demonstrated by immunoelectrophoresis with use of the sera of the test animals. Band A occurred between the 1st and 2nd weeks after inoculation and was, for a period, present in 100% of the sera of the test animals. Band A occurred separately or in conjunction with band B or B and C, and it persisted longer than these other two bands. Bands B and C appeared between the 3rd and 4th weeks and were found, by the end of the 5th week, in 35 and 15%, respectively, of the sera tested. They were never seen separately or together without the presence of band A. Band C disappeared by the end of the 6th week, and band B, by the 10th week, but band A was still present in 19% of the sera at the end of 10 weeks.<\/jats:p>","DOI":"10.1128\/jb.92.4.887-891.1966","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T01:55:49Z","timestamp":1578189349000},"page":"887-891","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Immunoelectrophoretic Studies of Sera from Rabbits Experimentally Infected with<i>Histoplasma capsulatum<\/i>"],"prefix":"10.1128","volume":"92","author":[{"given":"David M.","family":"Adamson","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George C.","family":"Cozad","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"368","DOI":"10.4049\/jimmunol.81.5.368","article-title":"A study of the whole yeast cell agglutination test in rabbits experimentally infected with Histoplasma capsulatum","volume":"81","author":"COZAD G. C.","year":"1958","unstructured":"COZAD , G. C. 1958 . A study of the whole yeast cell agglutination test in rabbits experimentally infected with Histoplasma capsulatum . J. Immunol. 81 : 368 - 375 .","journal-title":"J. Immunol."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"387","DOI":"10.4049\/jimmunol.85.4.387","article-title":"A capillary tube agglutination test for histoplasmosis","volume":"85","author":"COZAD G. C.","year":"1960","unstructured":"COZAD , G. C. , AND H. W. LARSH. 1960 . A capillary tube agglutination test for histoplasmosis . J. Immunol. 85 : 387 - 390 .","journal-title":"J. Immunol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.2307\/4585471","article-title":"Studies of the role of fungi in pulmonary disease; cross-reactions of histoplasmin","volume":"60","author":"EMMONS C. W.","year":"1945","unstructured":"EMMONS , C. W. , B. J. OLSON , A ND W . W. ELDRIDGE. 1945 . Studies of the role of fungi in pulmonary disease; cross-reactions of histoplasmin . Public Health Rept. 60 : 1383 - 1394 .","journal-title":"Public Health Rept."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1164\/arrd.1963.87.4.592","article-title":"A micro-method of the agargel precipitation test for histoplasmosis","volume":"87","author":"GOLDIN M.","year":"1963","unstructured":"GOLDIN , M. 1963 . A micro-method of the agargel precipitation test for histoplasmosis . Am. Rev. Respirat. Diseases 87 : 592 - 593 .","journal-title":"Am. Rev. Respirat. Diseases"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0006-3002(53)90233-9","article-title":"Methode permettant L'Etude conjuquee des propri 6tes electrophoretiques et immunochimiques D'un melange de proteines. Application au serum sanguin","volume":"10","author":"GRABAR P.","year":"1953","unstructured":"GRABAR , P. , AND C. A. WILLIAM s, JR. 1953 . Methode permettant L'Etude conjuquee des propri 6tes electrophoretiques et immunochimiques D'un melange de proteines. Application au serum sanguin . Biochim. Biophys. Acta 10 : 193 - 194 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_6","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1542\/peds.22.4.616","article-title":"Diagnosis of histoplasmosis using precipitin reaction to agar-gel","volume":"22","author":"HEINER AS C","year":"1958","unstructured":"HEINER , DOUGL AS C . 1958 . Diagnosis of histoplasmosis using precipitin reaction to agar-gel . Pediatrics 22 : 616 - 627 .","journal-title":"Pediatrics"},{"key":"p_7","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1699-0463.1949.tb00751.x","article-title":"Antigen-antibody reactions in gels","volume":"26","author":"OUCHTERLONY","year":"1949","unstructured":"OUCHTERLONY , 0. 1949 . Antigen-antibody reactions in gels . Acta Pathol. Microbiol Scand. 26 : 507 - 515 .","journal-title":"Acta Pathol. Microbiol Scand."},{"key":"p_8","first-page":"259","article-title":"Precipitins in human histoplasmosis","volume":"43","author":"SALVIN S. B.","year":"1954","unstructured":"SALVIN , S. B. , AND M. L. FURCOLOW. 1954 . Precipitins in human histoplasmosis . J. Lab. Clin. Med. 43 : 259 - 274 .","journal-title":"J. Lab. Clin. Med."},{"key":"p_9","first-page":"845","article-title":"Evaluation of the agar-plate precipitin test for histoplasmosis","volume":"84","author":"SCHUBERT J. H.","year":"1961","unstructured":"SCHUBERT , J. H. , H. J. LYNCH , JR. , AND L. AJELLO. 1961 . Evaluation of the agar-plate precipitin test for histoplasmosis . Am. Rev. Respirat. Diseases 84 : 845 - 849 .","journal-title":"Am. Rev. Respirat. Diseases"},{"key":"p_10","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1042\/bj0310313","article-title":"Electrophoresis of serum globulin","volume":"31","author":"TI LIUS","year":"1937","unstructured":"TI sE LIUS , A. 1937 . Electrophoresis of serum globulin . Biochem. J. 31 : 313 - 317 .","journal-title":"Biochem. J."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.92.4.887-891.1966","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.92.4.887-891.1966","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T18:44:21Z","timestamp":1695581061000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.92.4.887-891.1966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1966,10]]},"references-count":10,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1966,10]]}},"alternative-id":["10.1128\/jb.92.4.887-891.1966"],"URL":"https:\/\/doi.org\/10.1128\/jb.92.4.887-891.1966","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1966,10]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}