{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T05:28:32Z","timestamp":1759814912038},"reference-count":39,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1968,3,1]],"date-time":"1968-03-01T00:00:00Z","timestamp":-57974400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1968,3]]},"abstract":"<jats:p><jats:italic>Hydrogenomonas facilis<\/jats:italic>and<jats:italic>H. eutropha<\/jats:italic>cultured in fructose medium retained high levels of ribulose-1,5-diphosphate carboxylase only when the following conditions were fulfilled: low aeration, FeCl<jats:sub>3<\/jats:sub>addition to fructose medium, and cell harvest at or prior to mid-exponential phase of growth. Repression of carboxylase synthesis was demonstrated under conditions of high oxygen tension during growth of<jats:italic>H. eutropha<\/jats:italic>on fructose. Upon depletion of fructose in the growth medium, carboxylase activity fell abruptly in both organisms. The decline could not be attributed to a repressive mechanism. Rapid inactivation of carboxylase was promoted by transfer of mid-exponential-phase<jats:italic>H. eutropha<\/jats:italic>to a basal salts medium lacking fructose. During severe fructose starvation, N<jats:sub>2<\/jats:sub>, H<jats:sub>2<\/jats:sub>, 80% H<jats:sub>2<\/jats:sub>to 20% air, 2,4-dinitrophenol, actinomycin D, streptomycin, bicarbonate, and magnesium ion deficiency spared carboxylase. Nitrogen starvation or chloramphenicol afforded no protection during severe starvation. In vitro inactivation was also demonstrated in crude cell-free extracts from nonstarved, fructose-grown<jats:italic>H. eutropha<\/jats:italic>. Substrate bicarbonate protected against this loss. Inactivation of the carboxylase could not be demonstrated either by starvation of autotrophically grown cells or in autotrophic extracts. Autotrophic extracts mixed with heterotrophic extracts lost their carboxylase activity, but mixing with heterotrophic extracts that had been heated to 50 C resulted in no loss of activity. Mechanisms are proposed to accommodate these observations.<\/jats:p>","DOI":"10.1128\/jb.95.3.937-946.1968","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:06:32Z","timestamp":1578189992000},"page":"937-946","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Factors Affecting the Synthesis and Degradation of Ribulose-1,5-Diphosphate Carboxylase in<i>Hydrogenomonas facilis<\/i>and<i>Hydrogenomonas eutropha<\/i>"],"prefix":"10.1128","volume":"95","author":[{"given":"Glenn D.","family":"Kuehn","sequence":"first","affiliation":[{"name":"Department of Chemistry, Washington State University, Pullman, Washington 99163"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce A.","family":"McFadden","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Washington State University, Pullman, Washington 99163"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/0006-291X(65)90610-8","article-title":"Carbon dioxide fixation and carboxydismutase in Thiobacillus novellus","volume":"20","author":"ALEEM M. I.","year":"1965","unstructured":"ALEEM , M. I. H., AND E. HUANG. 1965 . Carbon dioxide fixation and carboxydismutase in Thiobacillus novellus . Biochem. Biophys. Res. Commun. 20 : 515 - 520 .","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1104\/pp.42.4.497","article-title":"Photosynthesis in Rhodospirillum rubrum. III. Metabolic control of reductive pentose phosphate and tricarboxylic acid cycle enzymes","volume":"42","author":"ANDERSON L.","year":"1967","unstructured":"ANDERSON , L. , AND R. C. FULLER. 1967 . Photosynthesis in Rhodospirillum rubrum. III. Metabolic control of reductive pentose phosphate and tricarboxylic acid cycle enzymes . Plant Physiol. 42 : 497 - 502 .","journal-title":"Plant Physiol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1085\/jgp.29.6.379","article-title":"Isolation of hexokinase from Baker's yeast","volume":"29","author":"BERGER L.","year":"1946","unstructured":"BERGER , L. , Am C. F. Co RI. 1946 . Isolation of hexokinase from Baker's yeast . J. Gen. Physiol. 29 : 379 - 391 .","journal-title":"J. Gen. Physiol."},{"key":"p_4","first-page":"259","article-title":"Biophysics","volume":"58","author":"BOLTON E. T.","year":"1959","unstructured":"BOLTON , E. T. , R. J. BRITTEN , D. B. Cow IE , B. J. MCCARTHY , K. MCQUILLEN , A ND R . B. ROBERTS. 1959 . Biophysics . Yearbook Carnegie Inst. 58 : 259 - 300 .","journal-title":"Yearbook Carnegie Inst."},{"key":"p_5","unstructured":"DAGLEY S. AND J. SYKEs. 1958. The influence of conditions of culture on certain soluble macromolecular components of Escherichia coli p. 62-69. In R. B. Roberts [ed.] Microsomal particles and protein synthesis Washington Academy of Sciences Washington D.C. Pergamon Press New York."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1146\/annurev.mi.16.100162.001325","article-title":"The endogenous metabolism of microorganisms","volume":"16","author":"DAWES E. A.","year":"1962","unstructured":"DAWES , E. A. , AND D. W. RIBBONS. 1962 . The endogenous metabolism of microorganisms . Ann. Rev. Microbiol. 16 : 241 - 264 .","journal-title":"Ann. Rev. Microbiol."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/BF00408709","article-title":"Uber das Wasserstoff aktivierende System von Hydrogenomonas H 16","volume":"54","author":"EBERHARDT E.","year":"1966","unstructured":"EBERHARDT , E. 1966 . Uber das Wasserstoff aktivierende System von Hydrogenomonas H 16 . Arch. Mikrobiol. 54 : 115 - 124 .","journal-title":"Arch. Mikrobiol."},{"key":"p_8","first-page":"969","article-title":"The proteins of green leaves. V. A cytoplasmic nucleoprotein from spinach and tobacco leaves","volume":"205","author":"EGGMAN L., S.","year":"1953","unstructured":"EGGMAN , L., S. J. SINGER , A ND S . G. WILDMAN. 1953 . The proteins of green leaves. V. A cytoplasmic nucleoprotein from spinach and tobacco leaves . J. Biol. Chem. 205 : 969 - 983 .","journal-title":"J. Biol. Chem."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/0006-3002(59)90179-9","article-title":"Latent enzymic activity of ribonucleoprtoein isolated from E. coli","volume":"36","author":"ELSON D.","year":"1959","unstructured":"ELSON , D. 1959 . Latent enzymic activity of ribonucleoprtoein isolated from E. coli . Biochim. Biophys. Acta 36 : 372 - 386 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_10","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1016\/S0021-9258(18)64142-0","article-title":"Carbon metabolism in Chromatium","volume":"236","author":"FULLER R. C.","year":"1961","unstructured":"FULLER , R. C. , R. M. SMILLIE , E. C. SISLER , A ND H . L. KORNBERG. 1961 . Carbon metabolism in Chromatium . J. Biol. Chem. 236 : 2140 - 2149 .","journal-title":"J. Biol. Chem."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JB.90.1.1-7.1965","article-title":"Enzymatic degradation of ribosomes during endogenous respiration of Pseudomonas aeruginosa","volume":"90","author":"GRONLUND A. F.","year":"1965","unstructured":"GRONLUND , A. F. , AND J. J. R. CAMPBELL. 1965 . Enzymatic degradation of ribosomes during endogenous respiration of Pseudomonas aeruginosa . J. Bacteriol. 90 : 1 - 7 .","journal-title":"J. Bacteriol."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1007\/BF00408416","article-title":"Abbau und Wiederverwertung von Poly-,B-hydroxybuttersaure durch Hydrogenomonas H 16","volume":"56","author":"HIPPE H.","year":"1967","unstructured":"HIPPE , H. 1967 . Abbau und Wiederverwertung von Poly-,B-hydroxybuttersaure durch Hydrogenomonas H 16 . Arch. Mikrobiol. 56 : 248 - 277 .","journal-title":"Arch. Mikrobiol."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1099\/00221287-33-3-445","article-title":"Ribulose diphosphate carboxylase in Thiorhodaceae","volume":"33","author":"HURLBERT R. E.","year":"1963","unstructured":"HURLBERT , R. E. , AND J. LASCELLES. 1963 . Ribulose diphosphate carboxylase in Thiorhodaceae . J. Gen. Microbiol. 33 : 445 - 458 .","journal-title":"J. Gen. Microbiol."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1073\/pnas.58.4.1703","article-title":"Regulation of glutamine synthetase. VIII. ATP: glutamine synthetase adenylyltransferase, an enzyme that catalyzes alterations in the regulatory properties of glutamine synthetase","author":"KINGDON H. S.","year":"1967","unstructured":"KINGDON , H. S. , B. M. SHAPIRO , A ND E . R. STADTMAN. 1967 . Regulation of glutamine synthetase. VIII. ATP: glutamine synthetase adenylyltransferase, an enzyme that catalyzes alterations in the regulatory properties of glutamine synthetase . Proc. Natl. Acad. Sci. U. S. 58: 1703 - 1710 .","journal-title":"Proc. Natl. Acad. Sci. U."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0006-3002(60)90117-7","article-title":"The influence of growth substrates on metabolic pathways in Micrococcus denitrificans","volume":"39","author":"KORNBERG H. L.","year":"1960","unstructured":"KORNBERG , H. L. , J. F. COLLINS , A ND D . BIGLEY. 1960 . The influence of growth substrates on metabolic pathways in Micrococcus denitrificans . Biochim. Biophys. Acta 39 : 9 - 24 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_16","unstructured":"LASCELLES J. 1962. Tetrapyrrole synthesis in 945"},{"key":"p_17","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"LOWRY H., N.","year":"1951","unstructured":"LOWRY , 0. H., N. J. ROSEBROUGH , A. L. FARR , A ND R . J. RANDALL. 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 .","journal-title":"J. Biol. Chem."},{"key":"p_18","unstructured":"MAHLER H. R. AND E. H. CoRDES. 1966. Biological Chemistry p. 594-595. Harper and Rowe Publishers Inc. New York."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1042\/bj0690110","article-title":"Turnover of protein in growing and non-growing populations of Escherichia coli","volume":"69","author":"MANDELSTAM J.","year":"1958","unstructured":"MANDELSTAM , J. 1958 . Turnover of protein in growing and non-growing populations of Escherichia coli . Biochem. J. 69 : 110 - 119 .","journal-title":"Biochem. J."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1128\/BR.24.3.289-308.1960","article-title":"The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation","volume":"24","author":"MANDELSTAM J.","year":"1960","unstructured":"MANDELSTAM , J. 1960 . The intracellular turnover of protein and nucleic acids and its role in biochemical differentiation . Bacteriol. Rev. 24 : 289 - 308 .","journal-title":"Bacteriol. Rev."},{"key":"p_21","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/0003-9861(57)90533-7","article-title":"The biochemistry of Hydrogenomonas. V. Factors affecting autotrophic fixation of carbon dioxide","volume":"66","author":"FADDEN B. A.","year":"1957","unstructured":"Mc FADDEN , B. A. , AND D. E. ATKINSON. 1957 . The biochemistry of Hydrogenomonas. V. Factors affecting autotrophic fixation of carbon dioxide . Arch. Biochem. Biophys. 66 : 16 - 22 .","journal-title":"Arch. Biochem. Biophys."},{"key":"p_22","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1128\/JB.86.5.971-977.1963","article-title":"Quantitative studies of the effect of organic substrates and 2,4-dinitrophenol on heterotrophic carbon dioxide fixation in Hydrogenomonasfacilis","volume":"86","author":"FADDEN B. A.","year":"1963","unstructured":"Mc FADDEN , B. A. , AND H. R. HOMANN. 1963 . Quantitative studies of the effect of organic substrates and 2,4-dinitrophenol on heterotrophic carbon dioxide fixation in Hydrogenomonasfacilis . J. Bacteriol. 86 : 971 - 977 .","journal-title":"J. Bacteriol."},{"key":"p_23","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1128\/JB.89.3.839-847.1965","article-title":"Characteristics and intermediates of short-term 14CO2 incorporation during ribose oxidation by Hydrogenomonas facilis","volume":"89","author":"FADDEN B. A.","year":"1965","unstructured":"Mc FADDEN , B. A. , AND H. R. HOMANN. 1965 . Characteristics and intermediates of short-term 14CO2 incorporation during ribose oxidation by Hydrogenomonas facilis . J. Bacteriol. 89 : 839 - 847 .","journal-title":"J. Bacteriol."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/0006-291X(65)90318-9","article-title":"Ribulose diphosphate carboxylase and CO2 incorporation in extracts of Hydrogenomonas facilis","volume":"19","author":"FADDEN B. A.","year":"1965","unstructured":"Mc FADDEN , B. A. , AND C. L. Tu. 1965 . Ribulose diphosphate carboxylase and CO2 incorporation in extracts of Hydrogenomonas facilis . Biochem. Biophys. Res. Commun. 19 : 728 - 733 .","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1128\/JB.93.3.886-893.1967","article-title":"Regulation of autotrophic and heterotrophic carbon dioxide fixation in Hydrogenomonas facilis","volume":"93","author":"FADDEN B. A.","year":"1967","unstructured":"Mc FADDEN , B. A. , AND C. L. Tu. 1967 . Regulation of autotrophic and heterotrophic carbon dioxide fixation in Hydrogenomonas facilis . J. Bacteriol. 93 : 886 - 893 .","journal-title":"J. Bacteriol."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0006-3002(59)90357-9","article-title":"Variations in the amount of ribonucleoprotein particles in E. coli","volume":"35","author":"MENDELSOHN J.","year":"1959","unstructured":"MENDELSOHN , J. , AND A. TISSIERES. 1959 . Variations in the amount of ribonucleoprotein particles in E. coli . Biochim. Biophys. Acta 35 : 248 - 250 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_27","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1021\/bi00890a006","article-title":"Partial purification and the enzymatic nature of fraction I protein of rice leaves","volume":"3","author":"MENDIOLA L.","year":"1964","unstructured":"MENDIOLA , L. , AND T. AKAZAWA. 1964 . Partial purification and the enzymatic nature of fraction I protein of rice leaves . Biochemistry 3 : 174 - 179 .","journal-title":"Biochemistry"},{"key":"p_28","unstructured":"MILTON R. F. AND W. A. WATERS. 1955. Methods of quantitative microanalysis p. 318-319. Edward Arnold Ltd. London England."},{"key":"p_29","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0304-4165(65)90349-1","article-title":"Heterogeneity of protein turnover in Escherichia coli","volume":"104","author":"PINE M. J.","year":"1965","unstructured":"PINE , M. J. 1965 . Heterogeneity of protein turnover in Escherichia coli . Biochim. Biophys. Acta 104 : 439 - 456 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_30","unstructured":"POLLOCK M. R. 1959. Induced formation of enzymes p. 619-680. In P. D. Boyer H. Lardy and K. Myrback [ed.] The enzymes vol. 1. Academic Press Inc. New York."},{"key":"p_31","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/0006-3002(59)90052-6","article-title":"Carboxydismutase activity in formate and oxalategrown Pseudomonas oxalaticus (Strain OX1)","volume":"31","author":"QUAYLE J. R.","year":"1959","unstructured":"QUAYLE , J. R. , AND D. B. KEECH. 1959 . Carboxydismutase activity in formate and oxalategrown Pseudomonas oxalaticus (Strain OX1) . Biochim. Biophys. Acta 31 : 587 - 588 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_32","unstructured":"REPASKE R. 1962. Nutritional requirements for 946 KUEHN AND MCFADDEN"},{"key":"p_33","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1104\/pp.41.5.885","article-title":"Regulation of photosynthetic capacity in Chlamydomonas mundana","volume":"41","author":"RUSSEL G. K.","year":"1966","unstructured":"RUSSEL , G. K. , AND M. GIBBS. 1966 . Regulation of photosynthetic capacity in Chlamydomonas mundana . Plant Physiol. 41 : 885 - 890 .","journal-title":"Plant Physiol."},{"key":"p_34","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/0005-2787(67)90669-7","article-title":"Effect of actinomycin D and mitomycin D on the degradation of deoxyribonucleic acid and polydeoxyribonucleotide by deoxyribonucleases and venom phosphodiesterase","volume":"145","author":"SARKAR N. K.","year":"1967","unstructured":"SARKAR , N. K. 1967 . Effect of actinomycin D and mitomycin D on the degradation of deoxyribonucleic acid and polydeoxyribonucleotide by deoxyribonucleases and venom phosphodiesterase . Biochim. Biophys. Acta 145 : 174 - 177 .","journal-title":"Biochim. Biophys. Acta"},{"key":"p_35","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1128\/JB.63.1.87-98.1952","article-title":"Growth and hydrogenase production of a new bacterium, Hydrogenomonas facilis","volume":"63","author":"SCHATZ A.","year":"1952","unstructured":"SCHATZ , A. , AND C. BOVELL , JR. 1952 . Growth and hydrogenase production of a new bacterium, Hydrogenomonas facilis . J. Bacteriol. 63 : 87 - 98 . J. BACrERIOL.","journal-title":"J. Bacteriol."},{"key":"p_36","doi-asserted-by":"crossref","first-page":"4609","DOI":"10.1016\/S0021-9258(18)96999-1","article-title":"Studies of the stability in vivo and in vitro of rat liver tryptophan pyrrolase","volume":"240","author":"SCHIMKE R. T.","year":"1965","unstructured":"SCHIMKE , R. T. , E. W. SWEENEY , A ND C . M. BERLIN. 1965 . Studies of the stability in vivo and in vitro of rat liver tryptophan pyrrolase . J. Biol. Chem. 240 : 4609 - 4620 .","journal-title":"J. Biol. Chem."},{"key":"p_37","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/cber.18870200144","article-title":"Notiz uber eine Fruchtzucker-reaction","volume":"20","author":"SELIWANOFF T.","year":"1887","unstructured":"SELIWANOFF , T. 1887 . Notiz uber eine Fruchtzucker-reaction . Chem. Ber. 20 : 181 - 182 .","journal-title":"Chem. Ber."},{"key":"p_38","unstructured":"STENT G. 1963. Molecular biology of bacterial viruses p. 153. W. H. Freeman and Co. San Francisco."},{"key":"p_39","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1016\/S0021-9258(18)91262-7","article-title":"The effect of oxygenation on the rate of digestion of human hemoglobins by carboxylpeptidases","volume":"239","author":"ZIT","year":"1964","unstructured":"ZIT o, R., E. ANTONINI , A ND J . WYMAN. 1964 . The effect of oxygenation on the rate of digestion of human hemoglobins by carboxylpeptidases . J. Biol. Chem. 239 : 1804 - 1808 .","journal-title":"J. Biol. Chem."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.95.3.937-946.1968","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.95.3.937-946.1968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T00:13:25Z","timestamp":1665360805000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.95.3.937-946.1968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,3]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1968,3]]}},"alternative-id":["10.1128\/jb.95.3.937-946.1968"],"URL":"https:\/\/doi.org\/10.1128\/jb.95.3.937-946.1968","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1968,3]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}