{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T23:03:05Z","timestamp":1769382185341,"version":"3.49.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1968,8,1]],"date-time":"1968-08-01T00:00:00Z","timestamp":-44755200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1968,8]]},"abstract":"<jats:p>A comparative study of the mycelial phase of<jats:italic>Paracoccidioides brasiliensis<\/jats:italic>and<jats:italic>Blastomyces dermatitidis<\/jats:italic>reveals that both fungi are very much alike, containing multiple nuclei and nuclear pores, mitochondria, ribosomes, scarce endoplasmic reticulum, intracytoplasmic membrane systems, glycogen, and vacuoles. Shadowed cell walls show fine fibrillar surfaces that contrast with those in the yeast phase. The intracytoplasmic membrane system is continuous with the plasma membrane and is similar to bacterial mesosomes. Granules with light cores and dark rims are observed in the plasma membrane. Live hyphae growing inside a dead hypha are found much more frequently in immersed cultures than in solid-medium cultures, suggesting that breakage of the hypha could elicit this phenomenon.<\/jats:p>","DOI":"10.1128\/jb.96.2.533-543.1968","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:11:10Z","timestamp":1578190270000},"page":"533-543","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Mycelial Phase of<i>Paracoccidioides brasiliensis<\/i>and<i>Blastomyces dermatitidis<\/i>: an Electron Microscope Study"],"prefix":"10.1128","volume":"96","author":[{"given":"Luis M.","family":"Carbonell","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Instituto Venezolano de Investigaciones Cientificas, Apartado 1827, Caracas, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaquin","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Instituto Venezolano de Investigaciones Cientificas, Apartado 1827, Caracas, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0022-2836(65)80236-4","article-title":"Electron microscope and low angle X-ray diffraction studies on outer segment membrane from the retina of the frog","volume":"14","author":"Blaise J. K.","year":"1965","unstructured":"Blaise , J. K. , M. Dewey , A. E. Blaurock , and C. R. Worthington . 1965 . Electron microscope and low angle X-ray diffraction studies on outer segment membrane from the retina of the frog . J. Mol. Biol. 14 : 143 - 152 .","journal-title":"J. Mol. Biol."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1083\/jcb.7.1.127","article-title":"Relations between basophilia and fine structure of cytoplasm in the fungus Allomyces macrogynus","volume":"7","author":"Blondel B.","year":"1960","unstructured":"Blondel , B. , and G. Turian . 1960 . Relations between basophilia and fine structure of cytoplasm in the fungus Allomyces macrogynus , Em. J. Biophys. Biochem. Cytol. 7 : 127 - 134 .","journal-title":"Em. J. Biophys. Biochem. Cytol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1080\/00275514.1963.12017998","article-title":"The ultrastructure and development of septa in hyphae of Rhizoctonia solani","volume":"55","author":"Bracker C. E.","year":"1963","unstructured":"Bracker , C. E. , Jr. , and E. E. Butler . 1963 . The ultrastructure and development of septa in hyphae of Rhizoctonia solani . Mycologia 55 : 35 - 58 .","journal-title":"Mycologia"},{"key":"p_4","unstructured":"Carbonell L. M. and L. Pollak. 1962. \"Myelin figures\" in yeast cultures of Paracoccidioides VOL. 96 1968 MYCELIA IN P. BRASILIENSIS AND B. DERMATITIDIS"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1007\/BF02051247","article-title":"Ultraestructura del Paracoccidioides brasiliensis en cultivos de la fase levaduriforme","volume":"19","author":"Carbonell L. M.","year":"1963","unstructured":"Carbonell , L. M. , and L. Pollak . 1963 . Ultraestructura del Paracoccidioides brasiliensis en cultivos de la fase levaduriforme . Mycopathol. Mycol. Appl. 19 : 184 - 204 .","journal-title":"Mycopathol. Mycol. Appl."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/JB.90.2.504-510.1965","article-title":"Transformation of mycelial and yeast forms of Paracoccidioides brasiliensis in cultures and in experimental inoculations","volume":"90","author":"Carbonell L. M.","year":"1965","unstructured":"Carbonell , L. M. , and J. Rodriguez . 1965 . Transformation of mycelial and yeast forms of Paracoccidioides brasiliensis in cultures and in experimental inoculations . J. Bacteriol. 90 : 504 - 510 .","journal-title":"J. Bacteriol."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1128\/JB.94.1.213-223.1967","article-title":"Cell wall changes during the budding process of Paracoccidioides brasiliensis and Blastomyces dermatitidis","volume":"94","author":"Carbonell L. M.","year":"1967","unstructured":"Carbonell , L. M. 1967 . Cell wall changes during the budding process of Paracoccidioides brasiliensis and Blastomyces dermatitidis . J. Bacteriol. 94 : 213 - 223 .","journal-title":"J. Bacteriol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1080\/00275514.1920.12016827","article-title":"The life history of Ascobolus magnificus: origin of the ascoscarp from two strains","volume":"12","author":"Dodge B.","year":"1920","unstructured":"Dodge , B. 0. 1920 . The life history of Ascobolus magnificus: origin of the ascoscarp from two strains . Mycologia 12 : 115 - 133 .","journal-title":"Mycologia"},{"key":"p_9","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1002\/j.1537-2197.1960.tb07145.x","article-title":"The intracellular membranes of Blastomyces dermatitidis","volume":"47","author":"Edwards G. A.","year":"1960","unstructured":"Edwards , G. A. , and M. R. Edwards . 1960 . The intracellular membranes of Blastomyces dermatitidis . Am. J. Botany 47 : 622 - 632 .","journal-title":"Am. J. Botany"},{"key":"p_10","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1128\/JB.94.4.1189-1205.1967","article-title":"Fine structure of Bacillus megaterium during synchronous growth","volume":"94","author":"Ellar D. J.","year":"1967","unstructured":"Ellar , D. J. , D. G. Lundgren , and R. A. Slepecky 1967 . Fine structure of Bacillus megaterium during synchronous growth . J. Bacteriol. 94 : 1189 - 1205 .","journal-title":"J. Bacteriol."},{"key":"p_11","unstructured":"Emmons C. W. C. H. Binford and J. P. Utz. 1963. Medical mycology p. 271. Lea and Febiger Philadelphia."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1083\/jcb.13.3.437","article-title":"A new epoxy embedment for electron microscopy","volume":"13","author":"Freeman J. M.","year":"1962","unstructured":"Freeman , J. M. , and B. 0. Spurlock . 1962 . A new epoxy embedment for electron microscopy . J. Cell Biol. 13 : 437 - 443 .","journal-title":"J. Cell Biol."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1093\/ajcp\/25.8_ts.0975","article-title":"A stain for fungi in tissue sections and smears using Gomori's methenamine silver nitrate technics","volume":"25","author":"Grocott R. G.","year":"1955","unstructured":"Grocott , R. G. 1955 . A stain for fungi in tissue sections and smears using Gomori's methenamine silver nitrate technics . Am. J. Clin. Pathol. 25 : 975 - 979 .","journal-title":"Am. J. Clin. Pathol."},{"key":"p_14","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/j.1469-185X.1965.tb00795.x","article-title":"Fine structure of fungi as revealed by electron microscopy","volume":"40","author":"Hawker L. E.","year":"1965","unstructured":"Hawker , L. E. 1965 . Fine structure of fungi as revealed by electron microscopy . Biol. Rev. 40 : 52 - 92 .","journal-title":"Biol. Rev."},{"key":"p_15","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1128\/jb.85.1.150-163.1963","article-title":"Formation of intracytoplasmic membrane system of mycobacteria related to cell division","volume":"85","author":"Imaeda T.","year":"1963","unstructured":"Imaeda , T. , and M. Ogura . 1963 . Formation of intracytoplasmic membrane system of mycobacteria related to cell division . J. Bacteriol. 85 : 150 - 163 .","journal-title":"J. Bacteriol."},{"key":"p_16","doi-asserted-by":"crossref","unstructured":"Kendrick W. B. and A. C. Molnar. 1965. A new Ceratocystis and its Verticicladiella imperfect stage associated with the bark beetle Dryo-543","DOI":"10.1139\/b65-005"},{"key":"p_17","first-page":"541","article-title":"Intrahyphal hyphae in \"clock","volume":"58","author":"Lowry R. J.","year":"1966","unstructured":"Lowry , R. J. , and A. S. Sussman . 1966 . Intrahyphal hyphae in \"clock \" mutants of Neurospora. Mycologia 58 : 541 - 548 .","journal-title":"mutants of Neurospora. Mycologia"},{"key":"p_18","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1083\/jcb.2.6.799","article-title":"Permanganate-a new fixative for electron microscope","volume":"2","author":"Luft J. H.","year":"1956","unstructured":"Luft , J. H. 1956 . Permanganate-a new fixative for electron microscope . J. Biophys. Biochem. Cytol. 2 : 799 - 802 .","journal-title":"J. Biophys. Biochem. Cytol."},{"key":"p_19","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/0014-4827(59)90139-9","article-title":"Continuity of plasma membrane and nuclear membrane","volume":"16","author":"McAlear J. H.","year":"1959","unstructured":"McAlear , J. H. , and G. A. Edwards . 1959 . Continuity of plasma membrane and nuclear membrane . Exptl. Cell Res. 16 : 689 - 692 .","journal-title":"Exptl. Cell Res."},{"key":"p_20","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1126\/science.156.3774.528","article-title":"Blastomyces dermatitidis: production of the sexual stage","volume":"156","author":"McDonough E. S.","year":"1967","unstructured":"McDonough , E. S. , and A. L. Lewis . 1967 . Blastomyces dermatitidis: production of the sexual stage . Science 156 : 528 - 529 .","journal-title":"Science"},{"key":"p_21","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1080\/00275514.1961.12017949","article-title":"Fine structure of mycota. 5 Lomasomes-previously uncharacterized hyphal structures","volume":"53","author":"Moore R. T.","year":"1961","unstructured":"Moore , R. T. , and J. M. McAlear . 1961 . Fine structure of mycota. 5 Lomasomes-previously uncharacterized hyphal structures . Micologia 53 : 194 - 200 .","journal-title":"Micologia"},{"key":"p_22","first-page":"263","article-title":"Fine structure of mycota. I. Electron microscopy of the Discomycete Ascodesmis","volume":"5","author":"Moore R. T.","year":"1963","unstructured":"Moore , R. T. 1963 . Fine structure of mycota. I. Electron microscopy of the Discomycete Ascodesmis . Nova Hedwigia 5 : 263 - 278 .","journal-title":"Nova Hedwigia"},{"key":"p_23","unstructured":"Moreau C. 1953. Les genres Sordaria et Pleurage p. 331. In Encyclopedie mycologique vol. 25. Paul Lechevalier Paris."},{"key":"p_24","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1083\/jcb.24.3.489","article-title":"Multiperforate septation, Woronin bodies and septal plugs in Fusarium","volume":"24","author":"Reichle R. E.","year":"1965","unstructured":"Reichle , R. E. , and J. V. Alexander . 1965 . Multiperforate septation, Woronin bodies and septal plugs in Fusarium . J. Cell Biol. 24 : 489 - 496 .","journal-title":"J. Cell Biol."},{"key":"p_25","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1083\/jcb.6.3.423","article-title":"Electron microscopy of Neurospora crassa mycelium","volume":"6","author":"Shatkin A. J.","year":"1959","unstructured":"Shatkin , A. J. , and E. L. Tatum . 1959 . Electron microscopy of Neurospora crassa mycelium . J. Biophys. Biochem. Cytol. 6 : 423 - 426 .","journal-title":"J. Biophys. Biochem. Cytol."},{"key":"p_26","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/BF02049924","article-title":"A model for rhythmic and temperatureindependent growth in clock mutants of Neurospora","volume":"25","author":"Sussman A. S.","year":"1965","unstructured":"Sussman , A. S. , T. L. Durkee , and R. J. Lowry . 1965 . A model for rhythmic and temperatureindependent growth in clock mutants of Neurospora . Mycopathol. Mycol. Appl. 25 : 381 - 396 .","journal-title":"Mycopathol. Mycol. Appl."},{"key":"p_27","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1128\/JB.71.4.450-453.1956","article-title":"Electron microscopical studies of ultrathin sections in Penicillium chrysogenum","volume":"71","author":"Tsuda S.","year":"1956","unstructured":"Tsuda , S. 1956 . Electron microscopical studies of ultrathin sections in Penicillium chrysogenum . J. Bacteriol. 71 : 450 - 453 .","journal-title":"J. Bacteriol."},{"key":"p_28","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/S0022-5320(66)80092-8","article-title":"Subunits in chloroplast membrane of Scenedesmus quadricanda","volume":"15","author":"Weir T. E.","year":"1966","unstructured":"Weir , T. E. , T. Bisalputra , and A. Harrison . 1966 . Subunits in chloroplast membrane of Scenedesmus quadricanda . J. Ultrastruct. Res. 15 : 38 - 56 .","journal-title":"J. Ultrastruct. Res."},{"key":"p_29","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1099\/00221287-40-3-401","article-title":"The role of lomasomes in wall formation in Penicillium vermiculatum","volume":"40","author":"Wilsenach R.","year":"1965","unstructured":"Wilsenach , R. , and M. Kessel . 1965 . The role of lomasomes in wall formation in Penicillium vermiculatum . J. Gen. Microbiol. 40 : 401 - 404 .","journal-title":"J. Gen. Microbiol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.96.2.533-543.1968","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.96.2.533-543.1968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T00:14:28Z","timestamp":1665360868000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.96.2.533-543.1968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,8]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1968,8]]}},"alternative-id":["10.1128\/jb.96.2.533-543.1968"],"URL":"https:\/\/doi.org\/10.1128\/jb.96.2.533-543.1968","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1968,8]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}