{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:28:29Z","timestamp":1756996109803},"reference-count":13,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1968,12,1]],"date-time":"1968-12-01T00:00:00Z","timestamp":-34214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1968,12]]},"abstract":"<jats:p>\n            The relative ability of the calcium chelates of calcium disodium ethylenediaminetetraacetate (EDTA) and calcium trisodium ethylenetriaminepentaacetate (DTPA) to protect mice against lethal doses of\n            <jats:italic>Clostridium perfringens<\/jats:italic>\n            \u03b1-toxin was investigated. Their protective ability was assayed by the increase in survival time of mice which had been given large doses of toxin, and by determining the median protective dose of chelate that would protect mice against toxin at a minimum lethal dose of two. In both assay procedures, intraperitoneal, intravenous, and intracutaneous injections of toxin were utilized, and with each toxin injection route the protective ability of the chelate was determined with the three routes of injection. DTPA was 10 to 20 times more effective than EDTA with both types of assay procedure and with all injection routes. DTPA may be superior to EDTA as a protective agent not only because it binds zinc to a greater extent, but also because of its greater retention in the body and its ability to gain entrance into cells. It appears that DTPA may be of value as a therapeutic agent in gas-gangrene.\n          <\/jats:p>","DOI":"10.1128\/jb.96.6.1925-1930.1968","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:16:09Z","timestamp":1578190569000},"page":"1925-1930","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Chelates in Chemotherapy of Experimental Gas-Gangrene Toxemia"],"prefix":"10.1128","volume":"96","author":[{"given":"Kenneth L.","family":"Lynch","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merwin","family":"Moskowitz","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","unstructured":"l. Basil B. 1963. Statistical evaluation in chemotherapy p. 57-79. hI R. J. Schnitzer and F. Hawking. (ed.) Experimental chemotherapy vol. 1. Academic Press Inc. New York.","DOI":"10.1016\/B978-1-4832-3178-5.50008-3"},{"key":"p_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1128\/JB.82.2.275-283.1961","article-title":"Fate of partially purified C'4-labeled toxin of Clostridium perfringen 7s","volume":"82","author":"Ellner P. D.","year":"1961","unstructured":"Ellner , P. D. 1961 . Fate of partially purified C'4-labeled toxin of Clostridium perfringen 7s . J. Bacteriol. 82 : 275 - 283 .","journal-title":"J. Bacteriol."},{"key":"p_3","unstructured":"Finney D. J. 1952. Probit analysis 2nd ed. Cambridge Univ. Press Cambridge England."},{"key":"p_4","first-page":"77","article-title":"Morphological and biochemical effects of Clostridium perfringens alpha toxin on muscle mitochondria","volume":"50","author":"Grossman W.","year":"1967","unstructured":"Grossman , W. , D. H. Heitkamp , and B. Sacktor . 1967 . Morphological and biochemical effects of Clostridium perfringens alpha toxin on muscle mitochondria . Am. J. Pathol. 50 : 77 .","journal-title":"Am. J. Pathol."},{"key":"p_5","doi-asserted-by":"crossref","unstructured":"Lynch K. L. and M. Moskowitz. 1968. Relationship of route of inoculation and nature of toxin preparation to bioassay of Clostridium J. BACTERIOL.","DOI":"10.1128\/jb.96.6.1920-1924.1968"},{"key":"p_6","first-page":"57","article-title":"On the biochemical mechanism of action of gas-gangrene toxins","volume":"5","author":"MacFarlane M. G.","year":"1955","unstructured":"MacFarlane , M. G. 1955 . On the biochemical mechanism of action of gas-gangrene toxins . Symp. Soc. Gen. Microbiol. 5 : 57 - 77 .","journal-title":"Symp. Soc. Gen. Microbiol."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1126\/science.123.3207.1077","article-title":"Protection against Clostridium perfringens type A toxin by a metal-chelating compound","volume":"123","author":"Moskowitz M.","year":"1956","unstructured":"Moskowitz , M. , M. W. Deverell , and R. Mc-Kinney . 1956 . Protection against Clostridium perfringens type A toxin by a metal-chelating compound . Science 123 : 1077 .","journal-title":"Science"},{"key":"p_8","doi-asserted-by":"crossref","first-page":"706","DOI":"10.3181\/00379727-92-22587","article-title":"Metal chelates as therapeutic and detoxifying agents in gas-gangrene","volume":"92","author":"Moskowitz M.","year":"1956","unstructured":"Moskowitz , M. 1956 . Metal chelates as therapeutic and detoxifying agents in gas-gangrene . Proc. Soc. Exptl. Biol. Med. 92 : 706 .","journal-title":"Proc. Soc. Exptl. Biol. Med."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/181550a0","article-title":"Mode of action of chelates in protecting against Clostridium perfringens toxin","volume":"181","author":"Moskowitz M.","year":"1958","unstructured":"Moskowitz , M. 1958 . Mode of action of chelates in protecting against Clostridium perfringens toxin . Nature 181 : 550 .","journal-title":"Nature"},{"key":"p_10","unstructured":"Moskowitz M. 1960. Protection against Clostridiumn perfringens toxin by metal-binding compounds p. 205. In M. J. Seven and L. A. Johnson (ed.) Metal-binding in medicine. J. B. Lippincott Co. Philadelphia."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1146\/annurev.me.14.020163.001135","article-title":"Synthetic chelating agents in clinical medicine","volume":"14","author":"Pullman T. N.","year":"1963","unstructured":"Pullman , T. N. , A. R. Lavender , and N. Forland . 1963 . Synthetic chelating agents in clinical medicine . Ann. Rev. Med. 14 : 175 - 194 .","journal-title":"Ann. Rev. Med."},{"key":"p_12","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1128\/JB.72.1.108-114.1956","article-title":"The linear representation of dosage-response curves in microbial-antibiotic assays","volume":"72","author":"Treffers H. P.","year":"1956","unstructured":"Treffers , H. P. 1956 . The linear representation of dosage-response curves in microbial-antibiotic assays . J. Bacteriol. 72 : 108 - 114 .","journal-title":"J. Bacteriol."},{"key":"p_13","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1111\/j.1749-6632.1960.tb20040.x","article-title":"The influence of the physiologic disposition of chelates on their use in medicine","volume":"88","author":"Weiner M.","year":"1960","unstructured":"Weiner , M. 1960 . The influence of the physiologic disposition of chelates on their use in medicine . Ann. N. Y. Acad. Sci. 88 : 426 - 434 .","journal-title":"Ann. N. Y. Acad. Sci."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.96.6.1925-1930.1968","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.96.6.1925-1930.1968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T16:50:03Z","timestamp":1627577403000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.96.6.1925-1930.1968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,12]]},"references-count":13,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1968,12]]}},"alternative-id":["10.1128\/jb.96.6.1925-1930.1968"],"URL":"https:\/\/doi.org\/10.1128\/jb.96.6.1925-1930.1968","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1968,12]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}