{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T11:02:12Z","timestamp":1648897332100},"reference-count":11,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1969,2,1]],"date-time":"1969-02-01T00:00:00Z","timestamp":-28857600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1969,2]]},"abstract":"<jats:p>\n            Clinical responses of patients with blastomycosis to treatment with hamycin have been variable. An explanation for this was sought in a series of studies in which in vitro and in vivo susceptibilities to hamycin of five strains of\n            <jats:italic>Blastomyces dermatitidis<\/jats:italic>\n            were compared. Minimal inhibitory concentrations of hamycin for the five strains indicated uniformly high levels of in vitro susceptibility (0.008 to 0.016 \u03bcg\/ml). In vivo activity was measured in infected mice treated intraperitoneally for a period of 28 days with doses of the drug ranging from 0.001 to 0.030 mg per mouse. Significant differences in response to treatment among the five strains were noted (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001), and protective doses were found to vary from 0.001 to &gt;0.030 mg per mouse per day. Further observations of infected mice after treatment revealed marked rates of relapsing infection, and several strains caused death. Persistent inapparent infections were also detected on culture of selected organs. Toxicity due to hamycin alone was not observed. These results suggest that variations in clinical responses to hamycin therapy in treatment of blastomycosis reflect differences in pathogenesis and host response in vivo to the infecting organism rather than differences in susceptibility of\n            <jats:italic>B. dermatitidis<\/jats:italic>\n            to hamycin.\n          <\/jats:p>","DOI":"10.1128\/jb.97.2.481-487.1969","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:13:41Z","timestamp":1578190421000},"page":"481-487","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["In Vitro and In Vivo Activity of Hamycin Against\n            <i>Blastomyces dermatitidis<\/i>"],"prefix":"10.1128","volume":"97","author":[{"given":"Smith","family":"Shadomy","sequence":"first","affiliation":[{"name":"Division of Allergy and Infectious Diseases, and Departments of Medicine and Biometry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Giles M.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, and Departments of Medicine and Biometry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Jean","family":"Shadomy","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, and Departments of Medicine and Biometry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Utz","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, and Departments of Medicine and Biometry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine","family":"Gamble","sequence":"additional","affiliation":[{"name":"Division of Allergy and Infectious Diseases, and Departments of Medicine and Biometry, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23219"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","unstructured":"l. Batson H. C. 1956. An introduction to statistics in the medical sciences. Burgess Publishing Co. Minneapolis Minn."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"166","DOI":"10.3181\/00379727-117-29525","article-title":"In vivo activity of colloidal hamycin, an antifungal antibiotic","volume":"117","author":"Bennett J. E.","year":"1964","unstructured":"Bennett , J. E. , T. W. Williams , W. Piggott , and C. W. Emmons . 1964 . In vivo activity of colloidal hamycin, an antifungal antibiotic . Proc. Soc. Exptl. Biol. Med. 117 : 166 - 170 .","journal-title":"Proc. Soc. Exptl. Biol. Med."},{"key":"p_3","first-page":"139","article-title":"Hamycin, a new antifungal antibiotic. II. Isolation and chemical properties","volume":"3","author":"Bhate D. S.","year":"1961","unstructured":"Bhate , D. S. , G. R. Ambekar , K. K. Bhatnagar , and R. K. Hulyalkar . 1961 . Hamycin, a new antifungal antibiotic. II. Isolation and chemical properties . Hindustan Antibiot. Bull. 3 : 139 - 143 .","journal-title":"Hindustan Antibiot. Bull."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1128\/jb.83.2.219-227.1962","article-title":"Nutritional studies on the yeast phase of Blastomyces dermatitidis and B. brasiliensis","volume":"83","author":"Gilardi G. L.","year":"1962","unstructured":"Gilardi , G. L. , and N. C. Laffer . 1962 . Nutritional studies on the yeast phase of Blastomyces dermatitidis and B. brasiliensis . J. Bacteriol. 83 : 219 - 227 .","journal-title":"J. Bacteriol."},{"key":"p_5","first-page":"112","article-title":"Preliminary report on the fate of hamycin in animals","volume":"6","author":"Kaul P. N.","year":"1964","unstructured":"Kaul , P. N. 1964 . Preliminary report on the fate of hamycin in animals . Hindustan Antibiot. Bull. 6 : 112 - 113 .","journal-title":"Hindustan Antibiot. Bull."},{"key":"p_6","first-page":"41","article-title":"Hamycin in the treatment of Cryptococcus neoformans infection in mice (preliminary communication)","volume":"6","author":"Padhye A. A.","year":"1963","unstructured":"Padhye , A. A. , and M. J. Thirumalachar . 1963 . Hamycin in the treatment of Cryptococcus neoformans infection in mice (preliminary communication) . Hindustan Antibiot. Bull. 6 : 41 - 43 .","journal-title":"Hindustan Antibiot. Bull."},{"key":"p_7","first-page":"136","article-title":"Hamycin, a new antifungal antibiotic. I. Discovery and biological studies","volume":"3","author":"Thirumalachar M. J.","year":"1961","unstructured":"Thirumalachar , M. J. , S. K. Menon , and V. V. Bhatt . 1961 . Hamycin, a new antifungal antibiotic. I. Discovery and biological studies . Hindustan Antibiot. Bull. 3 : 136 - 138 .","journal-title":"Hindustan Antibiot. Bull."},{"key":"p_8","first-page":"506","article-title":"Hamycin: Chemotherapeutic studies in systemic mycoses of man","volume":"95","author":"Utz J. P.","year":"1967","unstructured":"Utz , J. P. , P. Witorsch , T. W. Williams , Jr. , C. W. Emmons , H. J. Shadomy , and W. Piggott . 1967 . Hamycin: Chemotherapeutic studies in systemic mycoses of man . Am. Rev. Respirat. Diseases 95 : 506 - 509 .","journal-title":"Am. Rev. Respirat. Diseases"},{"key":"p_9","unstructured":"Utz J. P. H. J. Shadomy and S. Shadomy. 1968. Clinical and laboratory studies of a new micronized preparation of hamycin in systemic mycoses in man. Antimicrobial Agents and Chemotherapy-1967 p. 113-117."},{"key":"p_10","unstructured":"Williams T. W. Jr. J. E. Bennett and C. W. Emmons. 1965. Chemotherapeutic and toxic activity of hamycin in experimental mycoses. Antimicrobial Agents and Chemotherapy-1964 p. 737-741."},{"key":"p_11","doi-asserted-by":"crossref","first-page":"481","DOI":"10.3181\/00379727-120-30567","article-title":"Hamycin treatment of experimental bLiatomycosis in mice","volume":"120","author":"Williams T. W.","year":"1965","unstructured":"Williams , T. W. , Jr. , and C. W. Emmons . 1965 . Hamycin treatment of experimental bLiatomycosis in mice . Proc. Soc. Exptl. Biol. Med. 120 : 481 - 484 .","journal-title":"Proc. Soc. Exptl. Biol. Med."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.97.2.481-487.1969","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.97.2.481-487.1969","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,4]],"date-time":"2021-09-04T03:02:46Z","timestamp":1630724566000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.97.2.481-487.1969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,2]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1969,2]]}},"alternative-id":["10.1128\/jb.97.2.481-487.1969"],"URL":"https:\/\/doi.org\/10.1128\/jb.97.2.481-487.1969","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1969,2]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}