{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T10:04:17Z","timestamp":1769421857866,"version":"3.49.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1969,2,1]],"date-time":"1969-02-01T00:00:00Z","timestamp":-28857600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1969,2]]},"abstract":"<jats:p>The number of tumors initiated by<jats:italic>Agrobacterium tumefaciens<\/jats:italic>strain B6 on primary pinto bean leaves was decreased when cells of an avirulent strain (IIBNV6) were included in the inoculum. With sufficient B6 cells to initiate ca. 50% of the maximal number of tumors per leaf, inhibition was detected at a 1:1 ratio of B6 to IIBNV6 cells and increased linearly with the logarithm of the number of IIBNV6. Varying the number of B6 in the presence of a constant number of IIBNV6 or varying the number of both, while maintaining a constant ratio of B6 to IIBNV6, showed that the inhibition was a function of the absolute concentration of each cell type. The data fit a one-particle dose response curve, which indicates that a single IIBNV6 cell can prevent tumor initiation by a single B6 cell. Inhibition was obtained with mixed inocula and when the addition of IIBNV6 preceded B6, but not when B6 preceded IIBNV6. Heat-inactivated IIBNV6 inhibited, as did ultraviolet or heat-inactivated B6. Several unrelated bacteria and certain strains of<jats:italic>Agrobacterium<\/jats:italic>failed to inhibit, whereas other related strains gave inhibition. Attachment of IIBNV6 to a specific would site, thus excluding B6 from the site, is proposed to account for these data. A specific complementary binding of a virulent bacterium to a host wound site exposed by the inoculation procedure is suggested as an essential early event in the crown-gall tumor initiation process.<\/jats:p>","DOI":"10.1128\/jb.97.2.620-628.1969","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:13:54Z","timestamp":1578190434000},"page":"620-628","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":193,"title":["Bacterial Attachment to a Specific Wound Site as an Essential Stage in Tumor Initiation by<i>Agrobacterium tumefaciens<\/i>"],"prefix":"10.1128","volume":"97","author":[{"given":"Barbara B.","family":"Lippincott","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Northwestern University, Evanston, Illinois 60201"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Lippincott","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Northwestern University, Evanston, Illinois 60201"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","first-page":"779","article-title":"Severity of bacterial wilt as influenced by ratio of virulent to avirulent cells of Pseudomonas solanacearum in inoculum","volume":"54","author":"Averre C. 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