{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T01:15:18Z","timestamp":1777338918933,"version":"3.51.4"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1969,4,1]],"date-time":"1969-04-01T00:00:00Z","timestamp":-23760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1969,4]]},"abstract":"<jats:p>\n            Some antitumor agents known to specifically inhibit certain tumor cell enzymes were examined for activity against glycolytic enzymes and growth of the insect trypanosomatid,\n            <jats:italic>Crithidia fasciculata<\/jats:italic>\n            . The cytoplasmic enzymes hexokinase, \u03b1-glycerophosphate dehydrogenase, malic dehydrogenase, and glucose-6-phosphate dehydrogenase were tested. Agaricic acid (2-hydroxy-1,2,3-nonadecane tricarboxylic acid) was highly inhibitory (50 to 100%) to malic and \u03b1-glycerophosphate dehydrogenases at \u223c3 \u00d7 10\n            <jats:sup>\u22125<\/jats:sup>\n            <jats:sc>m<\/jats:sc>\n            ; 2-(\n            <jats:italic>p<\/jats:italic>\n            -hydroxyphenyl)-2-phenylpropane (2 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            <jats:sc>m<\/jats:sc>\n            ), and 5,6-dichloro-2-benzoxazolinone (5 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            <jats:sc>m<\/jats:sc>\n            ) were less effective (50% inhibition) against them. The antiprotozoal agents primaquine (4 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            <jats:sc>m<\/jats:sc>\n            ) and Melarsoprol (8 \u00d7 10\n            <jats:sup>\u22124<\/jats:sup>\n            <jats:sc>m<\/jats:sc>\n            ) were 30 to 40% inhibitory. Agaricic acid, 2-(\n            <jats:italic>p<\/jats:italic>\n            -hydroxyphenyl)-2-phenylpropane, and 5,6-dichloro-2-benzoxazolinone inhibited growth of\n            <jats:italic>Crithidia<\/jats:italic>\n            at less than 10\n            <jats:sup>\u22124<\/jats:sup>\n            <jats:sc>m<\/jats:sc>\n            . Eight other test compounds from the Cancer Chemotherapy National Service Center (CCNSC) were not toxic to cell growth, although two (4-biphenylcarboxylic acid and 1-[\n            <jats:italic>p<\/jats:italic>\n            -chlorobenzyl]-2-ethyl-5-methyl-indole-3-acetic acid) inhibited\n            <jats:italic>Crithidia<\/jats:italic>\n            \u03b1-glycerophosphate dehydrogenase below 1 m\n            <jats:sc>m<\/jats:sc>\n            . All of the compounds used specifically inhibited cancer cell \u03b1-glycerophosphate dehydrogenase. The corresponding enzyme in pathogenic African trypanosomes is important in their terminal respiration.\n            <jats:italic>C. fasciculata<\/jats:italic>\n            may be useful in preliminary evaluation of chemotherapeutic agents as potential trypanocides.\n          <\/jats:p>","DOI":"10.1128\/jb.98.1.23-28.1969","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:15:38Z","timestamp":1578190538000},"page":"23-28","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Effects of Some Antitumor Agents on Growth and Glycolytic Enzymes of the Flagellate\n            <i>Crithidia<\/i>"],"prefix":"10.1128","volume":"98","author":[{"given":"Cyrus J.","family":"Bacchi","sequence":"first","affiliation":[{"name":"Haskins Laboratories, New York, New York 10017"},{"name":"Department of Pharmacology, Hahnemann Medical College, Philadelphia, Pennsylvania 19102"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward I.","family":"Ciaccio","sequence":"additional","affiliation":[{"name":"Haskins Laboratories, New York, New York 10017"},{"name":"Department of Pharmacology, Hahnemann Medical College, Philadelphia, Pennsylvania 19102"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lois E.","family":"Koren","sequence":"additional","affiliation":[{"name":"Haskins Laboratories, New York, New York 10017"},{"name":"Department of Pharmacology, Hahnemann Medical College, Philadelphia, Pennsylvania 19102"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","unstructured":"Bacchi C. 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