{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T09:53:20Z","timestamp":1695635600289},"reference-count":22,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1969,4,1]],"date-time":"1969-04-01T00:00:00Z","timestamp":-23760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1969,4]]},"abstract":"<jats:p>The inhibition of<jats:italic>Clostridium perfringens<\/jats:italic>\u03b1-toxin by ethylenediaminetetraacetate (EDTA) and diethylenetriaminepentacetate (DTPA) was studied utilizing three different in vitro assay procedures: diffusion on egg yolk-agar, disintegration of muscle sections, and manometric assay with partially purified lecithin as substrate. DTPA was 10 to 20 times more efficient as an inhibitor than EDTA in systems containing relatively large amounts of<jats:italic>calcium<\/jats:italic>; these observations were similar to those observed in previous in vivo protection studies. A number of other chelating agents were tested for their ability to inhibit \u03b1-toxin in vitro and protect mice against it; the chelating agents which were the most efficient in vitro inhibitors had the greatest in vivo protective ability.<\/jats:p>","DOI":"10.1128\/jb.98.1.29-35.1969","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:15:38Z","timestamp":1578190538000},"page":"29-35","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Relation of In Vitro Inhibition by Chelates of<i>Clostridium perfringens<\/i>\u03b1-Toxin to Their Ability to Protect Against Experimental Toxemia"],"prefix":"10.1128","volume":"98","author":[{"given":"Leah M. M.","family":"Senff","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merwin","family":"Moskowitz","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/path.1700710220","article-title":"The pathology of Clostridium welchii infection","volume":"71","author":"Aikat P. K.","year":"1956","unstructured":"Aikat , P. K. , and J. H. Dible . 1956 . The pathology of Clostridium welchii infection . J. Pathol. Bacteriol. 71 : 461 - 476 .","journal-title":"J. Pathol. 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L'action de l'acide ethylene-diamine-tetra-acetique (EDTA) sur l'intoxication et l'infection experimentale avec le Clostridium perfringens type A . Arch. Roumaines Pathol. Exptl. Microbiol. 22 : 153 - 158 .","journal-title":"Arch. Roumaines Pathol. Exptl. Microbiol."},{"key":"p_4","first-page":"153","article-title":"Action de l'acide ethylene-diamine-tetra-acetique (EDTA) et de ses complexes metalliques sur l'intoxication et l'infection experimentale avec le Clostridium perfringens type A","volume":"22","author":"Bittner J.","year":"1963","unstructured":"Bittner , J. , V. Voinesco , and D. Winter . 1963 . Action de l'acide ethylene-diamine-tetra-acetique (EDTA) et de ses complexes metalliques sur l'intoxication et l'infection experimentale avec le Clostridium perfringens type A . Arch. Roumaines Pathol. Exptl. Microbiol. 22 : 153 - 158 .","journal-title":"Arch. Roumaines Pathol. Exptl. Microbiol."},{"key":"p_5","unstructured":"Dawson R. M. C. 1964. Physicochemical aspects of phospholipase action p. 179-195. In R. M. C. Dawson and D. W. Rhodes (ed.) Metabolism and physiological significance of lipids. J. Wiley and Sons London."},{"key":"p_6","unstructured":"Foreman H. 1960. The pharmacology of some useful chelating agents. p. 92-94. In M. J. Seven (ed.) Metal-binding in medicine. J. B. Lippincott Co. Philadelphia."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/0005-2760(65)90080-9","article-title":"Action of phospholipase B on ultrasonically dispersed lecithin","volume":"106","author":"Kates M.","year":"1965","unstructured":"Kates , M. , J. R. Medeley , and J. L. Beare . 1965 . Action of phospholipase B on ultrasonically dispersed lecithin . Biochim. Biophys. Acta 106 : 630 - 634 .","journal-title":"Biochim. Biophys. 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