{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T13:28:26Z","timestamp":1699450106627},"reference-count":26,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[1969,6,1]],"date-time":"1969-06-01T00:00:00Z","timestamp":-18489600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1969,6]]},"abstract":"<jats:p>The major portion of the agglutinating and bactericidal activity of the sera of rabbits immunized with live<jats:italic>Vibrio cholerae<\/jats:italic>or with cholera vaccine was found in the \u03b3M fractions during the early stages of immunization. After 5 weeks or more, \u03b3G fractions accounted for more than half of the agglutinating activity. When late antibody was measured as the amount of protein precipitated by somatic antigens, nearly 3 times as much \u03b3G as \u03b3M was required for agglutination, and about 30 times as much \u03b3G as \u03b3M was required to kill 50% of a standard inoculum in the presence of complement. The ratio of vibriocidal to agglutinin titer of \u03b3G fractions at different stages of immunization was more variable than that of \u03b3M fractions. More complement was required for a vibriocidal effect by \u03b3G than by \u03b3M. Increasing the amount of complement decreased the amount of both \u03b3G and \u03b3M required to kill, but smaller amounts of \u03b3M required disproportionately larger amounts of complement. Less time was required by \u03b3M than by \u03b3G to kill 50% of the inoculum. Removal of the group-reactive antibody from anti-Ogawa serum and serum fractions by absorption with Inaba reduced the vibriocidal titer by more than one-half.<\/jats:p>","DOI":"10.1128\/jb.98.3.956-962.1969","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:17:54Z","timestamp":1578190674000},"page":"956-962","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Agglutinating and Bactericidal Properties of Fractions of Rabbit Anti-<i>Vibrio cholerae<\/i>Serum"],"prefix":"10.1128","volume":"98","author":[{"given":"Robert M.","family":"Pike","sequence":"first","affiliation":[{"name":"Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Texas 75235"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cleo H.","family":"Chandler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Texas Southwestern Medical School, Dallas, Texas 75235"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","first-page":"351","article-title":"A study of the technique for demonstration of 'O' and 'H' agglutinins in the serum of cholera patients","volume":"46","author":"De S. N.","year":"1958","unstructured":"De , S. N. , P. K. L. Choudhury , M. L. Ghose , and A. Mondal . 1958 . A study of the technique for demonstration of 'O' and 'H' agglutinins in the serum of cholera patients . Indian J. Med. Res. 46 : 351 - 358 .","journal-title":"Indian J. Med. Res."},{"key":"p_2","unstructured":"Feeley J. C. 1965. Comparison of vibriocidal and agglutinating antibody responses in cholera patients p. 220-221. Proceedings of the Cholera Research Symposium Honolulu U.S. Department of Health Education and Welfare."},{"key":"p_3","unstructured":"Feeley J. C. 1965. Passive protective activity of antisera in infant rabbits infected orally with Vibrio cholerae p. 231-235. Proceedings of the Cholera Research Symposium Honolulu U.S. Department of Health Education and Welfare."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"264","DOI":"10.4049\/jimmunol.89.2.264","article-title":"Vibriocidal antibody inhibition (VAI) analysis: a technique for the identification of the predominant vibriocidal antibodies in serum and for the detection and identification of Vibrio cholerae antigens","volume":"89","author":"Finkelstein R. A.","year":"1962","unstructured":"Finkelstein , R. A. 1962 . Vibriocidal antibody inhibition (VAI) analysis: a technique for the identification of the predominant vibriocidal antibodies in serum and for the detection and identification of Vibrio cholerae antigens . J. Immunol. 89 : 264 - 271 .","journal-title":"J. Immunol."},{"key":"p_5","first-page":"247","article-title":"A test of antigenicity for the selection of strains for inclusion in cholera vaccines","volume":"39","author":"Finkelstein R. 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Contribution a l'etude du complexe antigednique \"O\" des vibrions. II. Liberation selective spontan 6e de la paroi cellulaire de V. cholerae er V. El Tor avec perte de l'agglutinabilite 0. Isolement de l'agglutinog 6ne specifique. Ann. Inst. Pasteur 102:309-327."},{"key":"p_8","unstructured":"Gallut J. 1965. Antigenic structure of vibrios p. 235-243. Proceedings of the Cholera Research Symposium Honolulu U.S. Department of Health Education and Welfare."},{"key":"p_9","first-page":"143","article-title":"Serologic diagnosis of cholera","volume":"8","author":"Goodner K.","year":"1960","unstructured":"Goodner , K. , H. L. Smith , and H. Stempen . 1960 . Serologic diagnosis of cholera . J. Albert Einstein Med. Center 8 : 143 - 147 .","journal-title":"J. Albert Einstein Med. Center"},{"key":"p_10","first-page":"11","article-title":"Studies on the immune globulins of human serum. II. 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