{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:54:03Z","timestamp":1776300843671,"version":"3.50.1"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[1969,8,1]],"date-time":"1969-08-01T00:00:00Z","timestamp":-13219200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1969,8]]},"abstract":"\n Physarum flavicomum, P. polycephalum<\/jats:italic>\n , and\n P. rigidum<\/jats:italic>\n grew at\n p<\/jats:italic>\n H 4.2 in a medium composed of mineral salts, glucose, biotin, thiamine, hematin, and four amino acids. Important differences in\n p<\/jats:italic>\n H tolerance were noted among the species. The minimal medium of\n P. flavicomum<\/jats:italic>\n and\n P. polycephalum<\/jats:italic>\n contained the amino acids methionine, glycine, and arginine, but valine was also required by\n P. rigidum<\/jats:italic>\n . Starting with an inoculum of about 0.3 mg of protein per 25 ml of minimal medium,\n P. flavicomum<\/jats:italic>\n and\n P. polycephalum<\/jats:italic>\n grew to 23 mg and\n P. rigidum<\/jats:italic>\n to 12 mg of protein per 25 ml in 3, 2, and 4 weeks, respectively.\n P. flavicomum<\/jats:italic>\n and\n P. polycephalum<\/jats:italic>\n grew with valine or leucine replacing arginine in the minimal medium but the growth yields and growth rates were decreased. All three species utilized homocysteine thiolactone in the minimal media in place of methionine. Serine adequately replaced glycine for\n P. rigidum<\/jats:italic>\n but was inhibitory in the minimal medium of\n P. flavicomum<\/jats:italic>\n or\n P. polycephalum<\/jats:italic>\n unless homocysteine thiolactone also replaced methionine. Growth rates of all three organisms were increased in the presence of seven amino acids (original four plus leucine, lysine, and isoleucine).\n <\/jats:p>","DOI":"10.1128\/jb.99.2.531-534.1969","type":"journal-article","created":{"date-parts":[[2020,1,5]],"date-time":"2020-01-05T02:19:32Z","timestamp":1578190772000},"page":"531-534","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Growth of\n Physarum flavicomum<\/i>\n and\n Physarum rigidum<\/i>\n in Chemically Defined Minimal Media"],"prefix":"10.1128","volume":"99","author":[{"suffix":"Jr.","given":"Henry R.","family":"Henney","sequence":"first","affiliation":[{"name":"Department of Biology, University of Houston, Houston, Texas 77004"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Lynch","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Houston, Houston, Texas 77004"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","unstructured":"Alexopoulos C. J. 1966. Morphogenesis in the myxomycetes p. 211-234. In G. Ainsworth and A. Sussman (ed.) The fungi vol. 2. Academic Press Inc. New York."},{"key":"p_2","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1128\/JB.92.4.1028-1035.1966","article-title":"Methionine metabolism of the myxomycete Physarum polycephalum","volume":"92","author":"Daniel J. W.","year":"1966","unstructured":"Daniel , J. W. , and K. Babcock . 1966 . Methionine metabolism of the myxomycete Physarum polycephalum . J. Bacteriol. 92 : 1028 - 1035 .","journal-title":"J. Bacteriol."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1128\/jb.86.2.324-331.1963","article-title":"Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum","volume":"86","author":"Daniel J. W.","year":"1963","unstructured":"Daniel , J. W. , K. L. Babcock , A. H. Sievert , and H. P. Rusch . 1963 . Organic requirements and synthetic media for growth of the myxomycete Physarum polycephalum . J. Bacteriol. 86 : 324 - 331 .","journal-title":"J. Bacteriol."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1128\/JB.84.5.1104-1110.1962","article-title":"Hematinrequiring plasmodial myxomycete","volume":"84","author":"Daniel J. W.","year":"1962","unstructured":"Daniel , J. W. , J. Kelley , and H. P. Rusch . 1962 . Hematinrequiring plasmodial myxomycete . J. Bacteriol. 84 : 1104 - 1110 .","journal-title":"J. Bacteriol."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1099\/00221287-25-1-47","article-title":"The pure culture of Physarum polycephalum on a partially defined soluble medium","volume":"25","author":"Daniel J. W.","year":"1961","unstructured":"Daniel , J. W. , and H. P. Rusch . 1961 . The pure culture of Physarum polycephalum on a partially defined soluble medium . J. Gen. Microbiol. 25 : 47 - 59 .","journal-title":"J. Gen. Microbiol."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1099\/00221287-53-3-333","article-title":"Nutritional requirements for the growth in pure culture of the myxomycete Physarum rigidum and related species","volume":"53","author":"Henney H. R.","year":"1968","unstructured":"Henney , H. R. , Jr. , and M. R. Henney . 1968 . Nutritional requirements for the growth in pure culture of the myxomycete Physarum rigidum and related species . J. Gen. Microbiol. 53 : 333 - 339 .","journal-title":"J. Gen. Microbiol."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1099\/00221287-53-3-321","article-title":"The mating-type systems of the myxomycetes Physarum rigidum and P. flavicomum","volume":"53","author":"Henney M. R.","year":"1968","unstructured":"Henney , M. R. , and H. R. Henney , Jr. 1968 . The mating-type systems of the myxomycetes Physarum rigidum and P. flavicomum . J. Gen. Microbiol. 53 : 321 - 332 .","journal-title":"J. Gen. Microbiol."},{"key":"p_8","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1128\/JB.98.1.249-255.1969","article-title":"Characterization of ribosomes from the myxomycete Physarum rigidum grown in pure culture","volume":"98","author":"Henney H. R.","year":"1969","unstructured":"Henney , H. R. , Jr. , and D. Jungkind . 1969 . Characterization of ribosomes from the myxomycete Physarum rigidum grown in pure culture . J. Bacteriol. 98 : 249 - 255 .","journal-title":"J. Bacteriol."},{"key":"p_9","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1016\/S0021-9258(18)50164-2","article-title":"Distribution of folic acid derivatives in natural material. 1. Chicken liver folates","volume":"237","author":"Noronha J. M.","year":"1962","unstructured":"Noronha , J. M. , and M. Silverman . 1962 . Distribution of folic acid derivatives in natural material. 1. Chicken liver folates . J. Biol. Chem. 237 : 3299 - 3302 .","journal-title":"J. Biol. Chem."},{"key":"p_10","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1128\/JB.89.4.1145-1150.1965","article-title":"Glycine synthesis and metabolism in Escherichia coli","volume":"89","author":"Pizer L. I.","year":"1965","unstructured":"Pizer , L. I. 1965 . Glycine synthesis and metabolism in Escherichia coli . J. Bacteriol. 89 : 1145 - 1150 .","journal-title":"J. Bacteriol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.99.2.531-534.1969","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.99.2.531-534.1969","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T16:54:22Z","timestamp":1627577662000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.99.2.531-534.1969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,8]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1969,8]]}},"alternative-id":["10.1128\/jb.99.2.531-534.1969"],"URL":"https:\/\/doi.org\/10.1128\/jb.99.2.531-534.1969","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1969,8]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}