{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T22:01:53Z","timestamp":1792879313519,"version":"4.5.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tigecycline is one of the few remaining therapeutic options for extensively drug-resistant (XDR) Gram-negative bacilli (GNB). MICs of tigecycline to\n                    <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n                    have been reported to be elevated when determined by the Etest compared to determinations by the broth microdilution (BMD) method. The study aim was to compare the susceptibility of GNB to tigecycline by four different testing methods. GNB were collected from six health care systems (25 hospitals) in southeast Michigan from January 2010 to September 2011. Tigecycline MICs among\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    , carbapenem-resistant\n                    <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n                    (CRE), extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n                    , and susceptible\n                    <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n                    isolates were determined by Etest, BMD, Vitek-2, and MicroScan. Nonsusceptibility was categorized as a tigecycline MIC of \u22654 \u03bcg\/ml for both\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n                    . The study included 4,427 isolates: 2,065 ESBL-producing\n                    <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n                    , 1,105\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    , 888 susceptible\n                    <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n                    , and 369 CRE isolates. Tigecycline nonsusceptibility among\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    isolates was significantly more common as determined by Etest compared to that determined by BMD (odds ratio [OR], 10.3;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), MicroScan (OR, 12.4;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), or Vitek-2 (OR, 9.4;\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001). These differences were not evident with the other pathogens. Tigecycline MICs varied greatly according to the\n                    <jats:italic>in vitro<\/jats:italic>\n                    testing methods among\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    isolates. Etest should probably not be used by laboratories for tigecycline MIC testing of\n                    <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n                    isolates, since MICs are significantly elevated with Etest compared to those determined by the three other methods.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00001-14","type":"journal-article","created":{"date-parts":[[2014,3,6]],"date-time":"2014-03-06T00:33:33Z","timestamp":1394066013000},"page":"1617-1621","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Major Variation in MICs of Tigecycline in Gram-Negative Bacilli as a Function of Testing Method"],"prefix":"10.1128","volume":"52","author":[{"given":"Dror","family":"Marchaim","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Detroit Medical Center, Wayne State University, Detroit, Michigan, USA"},{"name":"Division of Infectious Diseases, Assaf Harofeh Medical Center, Zerifin, Israel"},{"name":"Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M.","family":"Pogue","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Services, Detroit Medical Center, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oran","family":"Tzuman","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Assaf Harofeh Medical Center, Zerifin, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayoko","family":"Hayakawa","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Detroit Medical Center, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Lephart","sequence":"additional","affiliation":[{"name":"Department of University Laboratories, Detroit Medical Center, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Salimnia","sequence":"additional","affiliation":[{"name":"Department of University Laboratories, Detroit Medical Center, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Painter","sequence":"additional","affiliation":[{"name":"Department of University Laboratories, Detroit Medical Center, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus J.","family":"Zervos","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Henry Ford Health System, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura E.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Henry Ford Health System, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Beth","family":"Perri","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Henry Ford Health System, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Hartman","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Henry Ford Health System, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rama V.","family":"Thyagarajan","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Oakwood Healthcare System, Dearborn, Michigan, 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