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Fast identification is essential to ensure appropriate antimicrobial therapy. Therefore, the objective of this study was to develop a protocol to identify\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            from bronchoalveolar lavage fluid (BALf) with matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry MALDI-TOF MS and to determine the diagnostic accuracy in comparison with other techniques. BALf was obtained from 104 cattle, and the presence of\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            was determined in the following three ways: (i) rapid identification of\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            with MALDI-TOF MS (RIMM) (BALf was enriched and after 24, 48, and 72 h of incubation and was analyzed using MALDI-TOF MS), (ii) triplex real-time PCR for\n            <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Mycoplasma bovirhinis<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Mycoplasma dispar<\/jats:named-content>\n            , and (iii) 10-day incubation on selective-indicative agar.\n          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