{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T16:06:17Z","timestamp":1776960377837,"version":"3.51.4"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T00:00:00Z","timestamp":1493078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["96841"],"award-info":[{"award-number":["96841"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["64787"],"award-info":[{"award-number":["64787"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["UID: 85858"],"award-info":[{"award-number":["UID: 85858"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome","doi-asserted-by":"publisher","award":["WT104803"],"award-info":[{"award-number":["WT104803"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome","doi-asserted-by":"publisher","award":["WT084323"],"award-info":[{"award-number":["WT084323"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome","doi-asserted-by":"publisher","award":["WT098316"],"award-info":[{"award-number":["WT098316"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"publisher","award":["10218"],"award-info":[{"award-number":["10218"]}],"id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The utility of a line probe assay (Genotype MTBDR\n            <jats:italic>plus<\/jats:italic>\n            ) performed directly on 2-month sputa to monitor tuberculosis treatment response is unknown. We assessed if direct testing of 2-month sputa with MTBDR\n            <jats:italic>plus<\/jats:italic>\n            can predict 2-month culture conversion and long-term treatment outcome. Xpert MTB\/RIF-confirmed rifampin-susceptible tuberculosis cases were recruited at tuberculosis diagnosis and followed up at 2 and 5 to 6 months. MTBDR\n            <jats:italic>plus<\/jats:italic>\n            was performed directly on 2-month sputa and on all positive cultured isolates at 2 and 5 to 6 months. We also investigated the association of a positive direct MTBDR\n            <jats:italic>plus<\/jats:italic>\n            at 2 months with subsequent unsuccessful tuberculosis treatment outcome (failure\/death during treatment or subsequent disease recurrence). A total of 279 patients (62% of whom were HIV-1 coinfected) were recruited. Direct MTBDR\n            <jats:italic>plus<\/jats:italic>\n            at 2 months had a sensitivity of 78% (95% confidence interval [CI], 65 to 87) and specificity of 80% (95% CI, 74 to 84) to predict culture positivity at 2 months with a high negative predictive value of 93% (95% CI, 89 to 96). Inconclusive genotypic susceptibility results for both rifampin and isoniazid were seen in 26% of MTBDR\n            <jats:italic>plus<\/jats:italic>\n            tests performed directly on sputum. Compared to a reference of MTBDR\n            <jats:italic>plus<\/jats:italic>\n            performed on positive cultures, the false-positive resistance rate for direct testing of MTBDR\n            <jats:italic>plus<\/jats:italic>\n            on sputa was 4% for rifampin and 2% for isoniazid. While a positive 2-month smear was not significantly associated with an unsuccessful treatment outcome (adjusted odds ratio [aOR], 2.69; 95% CI, 0.88 to 8.21), a positive direct MTBDR\n            <jats:italic>plus<\/jats:italic>\n            at 2 months was associated with an unsuccessful outcome (aOR 2.87; 95% CI, 1.11 to 7.42). There is moderate utility of direct 2-month MTBDR\n            <jats:italic>plus<\/jats:italic>\n            to predict culture conversion at 2 months and also to predict an unfavorable outcome.\n          <\/jats:p>","DOI":"10.1128\/jcm.00025-17","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T01:40:57Z","timestamp":1488418857000},"page":"1508-1515","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Utility of Second-Generation Line Probe Assay (Hain MTBDR\n            <i>plus<\/i>\n            ) Directly on 2-Month Sputum Specimens for Monitoring Tuberculosis Treatment Response"],"prefix":"10.1128","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7830-1394","authenticated-orcid":false,"given":"Neesha","family":"Rockwood","sequence":"first","affiliation":[{"name":"Department of Medicine, Imperial College, London, United Kingdom"},{"name":"Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, Republic of South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Wojno","sequence":"additional","affiliation":[{"name":"National Health Laboratory Service, Groote Schuur Hospital, Cape Town, Republic of South Africa"},{"name":"Division of Microbiology, Department of Pathology, University of Cape Town, Cape Town, Republic of South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonas","family":"Ghebrekristos","sequence":"additional","affiliation":[{"name":"National Health Laboratory Service, Groote Schuur Hospital, Cape Town, Republic of South Africa"},{"name":"Division of Microbiology, Department of Pathology, University of Cape Town, Cape Town, Republic of South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark P.","family":"Nicol","sequence":"additional","affiliation":[{"name":"Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, Republic of South Africa"},{"name":"National Health Laboratory Service, Groote Schuur Hospital, Cape Town, Republic of South Africa"},{"name":"Division of Microbiology, Department of Pathology, University of Cape Town, Cape Town, Republic of South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graeme","family":"Meintjes","sequence":"additional","affiliation":[{"name":"Department of Medicine, Imperial College, London, United Kingdom"},{"name":"Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, Republic of South Africa"},{"name":"Department of Medicine, University of Cape Town, Cape Town, Republic of South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Imperial College, London, United Kingdom"},{"name":"Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, Republic of South Africa"},{"name":"Department of Medicine, University of Cape Town, Cape Town, Republic of South Africa"},{"name":"The Francis Crick Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62073-5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071116"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70071-2"},{"key":"e_1_3_3_5_2","volume-title":"Definitions and reporting framework for tuberculosis\u20132013 revision","author":"World Health Organization","year":"2014","unstructured":"World Health Organization. 2014. Definitions and reporting framework for tuberculosis\u20132013 revision. World Health Organization, Geneva, Switzerland. http:\/\/apps.who.int\/iris\/bitstream\/10665\/79199\/1\/9789241505345_eng.pdf. Accessed 16 September 2015."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139017"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05903-11"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01958-12"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01602-13"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00128-10"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00472-13"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(13)70119-X"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201312-2140OC"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.13.0853"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160062"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02501-08"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00025-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00025-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:02:45Z","timestamp":1646474565000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00025-17"}},"subtitle":[],"editor":[{"given":"Geoffrey A.","family":"Land","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1128\/JCM.00025-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00025-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"2017-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}