{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:27:48Z","timestamp":1790645268754,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100008535","name":"Calgary Laboratory Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008535","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009014","name":"Abbott Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Life-threatening infection in neonates due to group B\n            <jats:named-content content-type=\"genus-species\">Streptococcus<\/jats:named-content>\n            (GBS) is preventable by screening of near-term pregnant women and treatment at delivery. A total of 295 vaginal-rectal swabs were collected from women attending antepartum clinics in Calgary, Alberta, Canada. GBS colonization was detected by the standard culture method (Strep B Carrot Broth subcultured to blood agar with a neomycin disk) and compared to recovery with Strep Group B Broth (Dalynn Biologicals) subcultured to StrepB\n            <jats:italic>Select<\/jats:italic>\n            chromogenic medium (CM; Bio-Rad Laboratories) and the Fast-Track Diagnostics GBS real-time PCR (quantitative PCR [qPCR]) assay (Phoenix Airmid Biomedical Corp.) performed with broth-enriched samples and the Abbott\n            <jats:italic>m2000<\/jats:italic>\n            sp\/\n            <jats:italic>m2000<\/jats:italic>\n            rt system. A total of 62\/295 (21%) women were colonized with GBS; 58 (19.7%) cases were detected by standard culture, while CM and qPCR each found 61 (20.7%) cases. The qPCR and CM were similar in performance, with sensitivities, specificities, and positive and negative predictive values of 98.4 and 98.4%, 99.6 and 99.6%, 98.4 and 98.4%, and 99.6 and 99.6%, respectively, compared to routine culture. Both qPCR and CM would allow more rapid reporting of routine GBS screening results than standard culture. Although the cost per test was similar for standard culture and CM, the routine use of qPCR would cost approximately four times as much as culture-based detection. Laboratories worldwide should consider implementing one of the newer methods for primary GBS testing, depending on the cost limitations of different health care jurisdictions.\n          <\/jats:p>","DOI":"10.1128\/jcm.00043-17","type":"journal-article","created":{"date-parts":[[2017,4,27]],"date-time":"2017-04-27T00:40:29Z","timestamp":1493253629000},"page":"2137-2142","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Evaluation of StrepB\n            <i>Select<\/i>\n            Chromogenic Medium and the Fast-Track Diagnostics Group B Streptococcus (GBS) Real-Time PCR Assay Compared to Routine Culture for Detection of GBS during Antepartum Screening"],"prefix":"10.1128","volume":"55","author":[{"given":"Deirdre L.","family":"Church","sequence":"first","affiliation":[{"name":"Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada"},{"name":"Departments of Pathology &amp; Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Department of Medicine, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Baxter","sequence":"additional","affiliation":[{"name":"Department of Family Medicine, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracie","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Larios","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada"},{"name":"Departments of Pathology &amp; Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Department of Medicine, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel B.","family":"Gregson","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada"},{"name":"Departments of Pathology &amp; Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Department of Medicine, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000239267.42561.06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2010-2217"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318223bad2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2001.110308"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/322697"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200001063420103"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020205"},{"key":"e_1_3_2_9_2","unstructured":"Verani\nJR\nMcGee\nL\nSchrag\nSJ Division of Bacterial Diseases National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention (CDC). 2010. Prevention of perinatal group B streptococcal disease\u2014revised guidelines from CDC 2010. MMWR Recomm Rep59(RR-10):1\u201336. https:\/\/www.cdc.gov\/mmwr\/preview\/mmwrhtml\/rr5910a1.htm."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00557-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.11.002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.01.016"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03356-12"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/600303"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.06.016"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01734-10"},{"key":"e_1_3_2_17_2","volume-title":"Evaluation of a new screening medium for the detection of group B streptococci (GBS)","author":"Hopper C","year":"2013","unstructured":"Hopper C Oleksiuk M . 2013. Evaluation of a new screening medium for the detection of group B streptococci (GBS). Thermo-Fisher Scientific, Basingstoke, Hants, United Kingdom."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00221-10"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2011.01.014"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-285"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0000000000000299"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2011.04.001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02780-12"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/876103"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00043-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00043-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:02:31Z","timestamp":1646474551000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00043-17"}},"subtitle":[],"editor":[{"given":"Peter","family":"Gilligan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":23,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1128\/JCM.00043-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00043-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]},"assertion":[{"value":"2017-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-04-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-06-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}