{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:53:29Z","timestamp":1785412409140,"version":"3.56.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,6,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n We compared the performance of two commercial antimicrobial susceptibility testing (AST) systems for contemporary Enterobacterales strains using broth microdilution (BMD) as the reference standard with 2022 Clinical and Laboratory Standards Institute or current FDA breakpoints applied. Enterobacterales clinical isolates with BMD results were tested in parallel using VITEK 2 AST test cards (N802, XN15, bioM\u00e9rieux, Hazelwood, MO, USA) and MicroScan NM56 AST panels (Beckman Coulter, Sacramento, CA, USA). The 200 isolates (57\n Escherichia coli<\/jats:italic>\n , 55\n Klebsiella pneumoniae<\/jats:italic>\n , 21\n Enterobacter cloacae<\/jats:italic>\n complex, 18\n Serratia marcescens<\/jats:italic>\n , 12\n Proteus mirabilis<\/jats:italic>\n , 10\n Citrobacter koseri<\/jats:italic>\n , 9\n Citrobacter freundii<\/jats:italic>\n , 8\n Klebsiella oxytoca<\/jats:italic>\n , 5\n Klebsiella aerogenes<\/jats:italic>\n , 3\n Providencia stuartii<\/jats:italic>\n , and 2\n Morganella morganii<\/jats:italic>\n ) included 25% extended-spectrum beta-lactamase (ESBL), 23% carbapenem-resistant Enterobacterales (CRE), and 4.5% AmpC resistance phenotypes. For the 28 antimicrobial agents tested, essential agreement (EA, MIC within \u00b11 doubling dilution), categorical agreement (CA, same categorical interpretation: susceptible, intermediate, susceptible dose dependent, and resistant), and error rates were calculated using BMD as the reference standard. Time to results (TTR) was determined for each instrument for all 200 isolates. Hands-on time was assessed by timing two technologists each setting up six batches of five isolates on each system. Accuracy was similar between systems with an overall CA > 94% and EA \u2265 96%. The CA was \u226590% for most agents tested on both systems (exceptions were ampicillin-sulbactam, cefoxitin, minocycline, and nitrofurantoin). The MicroScan with Prompt inoculum preparation required less hands-on setup time than VITEK (1.29 vs 1.83 min\/isolate). The median instrument TTR was less for VITEK (11.7 vs 18 hours,\n P<\/jats:italic>\n < 0.001), yielding an overall faster turnaround time.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n There are limited data directly comparing the performance of commercial antimicrobial susceptibility testing systems for contemporary bacterial strains using the Clinical and Laboratory Standards Institute broth microdilution method as the reference standard and applying updated breakpoints. These data will hopefully encourage labs to perform the necessary verification or validation studies needed to implement current breakpoints and ensure antimicrobial resistance is detected.<\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/jcm.00048-25","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T21:12:11Z","timestamp":1746047531000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of MicroScan and VITEK 2 systems for susceptibility testing of Enterobacterales with updated breakpoints"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2349-320X","authenticated-orcid":true,"given":"Sandra S.","family":"Richter","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03zzw1w08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic","place":["Jacksonville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth L.","family":"Dominguez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03zzw1w08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic","place":["Jacksonville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron A.","family":"Hupp","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03zzw1w08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic","place":["Jacksonville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Griffis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03zzw1w08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic","place":["Jacksonville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn H.","family":"MacVane","sequence":"additional","affiliation":[{"name":"bioMerieux, Inc.","place":["Hazelwood, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00203-19"},{"key":"e_1_3_3_3_2","volume-title":"M52 guideline. Clinical and Laboratory Standards Institute","author":"Clinical and Laboratory Standards Institute","year":"2015","unstructured":"Clinical and Laboratory Standards Institute. 2015. Verification of commercial microbial identification and antimicrobial susceptibility testing systems. 1st ed. M52 guideline. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"1"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00139-18"},{"key":"e_1_3_3_5_2","unstructured":"Food and Drug Administration. 2017. FDA-recognized antimicrobial susceptibility test interpretive criteria. Food and Drug Administration Washington DC. Available from: https:\/\/www.fda.gov\/Drugs\/DevelopmentApprovalProcess\/DevelopmentResources\/ucm410971.htm. Retrieved 10 Dec 2024."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofac007"},{"key":"e_1_3_3_7_2","volume-title":"Approved standard M07-A11. Clinical and Laboratory Standards Institute","author":"Clinical and Laboratory Standards Institute","year":"2018","unstructured":"Clinical and Laboratory Standards Institute. 2018. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M07-A11. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofz004"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04673-22"},{"key":"e_1_3_3_10_2","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute","year":"2022","unstructured":"Clinical and Laboratory Standards Institute. 2022. Performance standards for antimicrobial susceptibility testing. 32nd ed. CLSI supplement M100.","edition":"32"},{"key":"e_1_3_3_11_2","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute","year":"2023","unstructured":"Clinical and Laboratory Standards Institute. 2023. Performance standards for antimicrobial susceptibility testing. 33rd ed. CLSI supplement M100.","edition":"33"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.08.024"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00788-21"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01649-19"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1479"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac688"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01617-22"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1159\/000512582"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02697-14"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00433-18"},{"key":"e_1_3_3_21_2","volume-title":"Manual of clinical microbiology","author":"Karlowsky JA","year":"2023","unstructured":"Karlowsky JA, Richter SS, Patel JB. 2023. Antimicrobial susceptibility testing systems. In Carroll et al (ed), Manual of clinical microbiology, 13th ed. American Society for Microbiology Press, Washington D.C.","edition":"13"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00111-10"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00048-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00048-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T09:00:54Z","timestamp":1749632454000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00048-25"}},"subtitle":[],"editor":[{"given":"Patricia J.","family":"Simner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,6,11]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6,11]]}},"alternative-id":["10.1128\/jcm.00048-25"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00048-25","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,11]]},"assertion":[{"value":"2025-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00048-25"}}