{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T23:12:39Z","timestamp":1786403559881,"version":"build-2736575974"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish<jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>var.<jats:italic>grubii<\/jats:italic>,<jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>var.<jats:named-content content-type=\"genus-species\">neoformans<\/jats:named-content>, and<jats:named-content content-type=\"genus-species\">Cryptococcus gattii<\/jats:named-content>according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.<\/jats:p>","DOI":"10.1128\/jcm.00064-13","type":"journal-article","created":{"date-parts":[[2013,3,28]],"date-time":"2013-03-28T02:27:18Z","timestamp":1364437638000},"page":"1920-1923","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var.<i>grubii<\/i>, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids"],"prefix":"10.1128","volume":"51","author":[{"given":"Xiaobo","family":"Feng","sequence":"first","affiliation":[{"name":"Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering, Tongji University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Ling","sequence":"additional","affiliation":[{"name":"Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering, Tongji University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqing","family":"Liao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Institute of Medical Mycology, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhirong","family":"Yao","sequence":"additional","affiliation":[{"name":"Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1301.060827"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0902.020246"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2008.00422.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.046672"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.1.28"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4815-4817.2004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1407.080122"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701824429"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.2008\/003665-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1002\/elps.200700903","article-title":"Hyperbranched rolling circle amplification as a rapid and sensitive method for species identification within the Cryptococcus species complex","volume":"29","author":"Kaocharoen S","year":"2008","unstructured":"KaocharoenS WangB TsuiKM TrillesL KongF MeyerW. 2008. 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