{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:31:05Z","timestamp":1785403865798,"version":"3.56.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["I01BX006885-01A1"],"award-info":[{"award-number":["I01BX006885-01A1"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"name":"American Rescue Plan Act funds"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2026,7,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Emergence of resistance to fidaxomicin is a threat to current treatment algorithms for\n                      <jats:italic toggle=\"yes\">Clostridioides difficile<\/jats:italic>\n                      infection (CDI), but current surveillance methods are labor-intensive and expensive. We tested the hypothesis that\n                      <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                      Brucella agar (CDBA) media supplemented with 1 \u00b5g\/mL fidaxomicin (CDBA-F1) would provide a sensitive, selective, and cost-effective method to screen stool specimens for\n                      <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                      isolates with reduced fidaxomicin susceptibility (minimum inhibitory concentration\n                      <jats:underline>&gt;<\/jats:underline>\n                      2 \u00b5g\/mL). Clinical stool specimens testing positive for\n                      <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">N<\/jats:italic>\n                      = 126) were plated on CDBA and CDBA-F1;\n                      <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                      isolates recovered were tested for fidaxomicin susceptibility using agar dilution. The sensitivity of CDBA-F1 in comparison to CDBA was 100% for recovery of isolates with reduced fidaxomicin susceptibility (eight of eight detected). Breakthrough growth of stool microbiota was common, but most breakthrough organisms were easily distinguishable from\n                      <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                      by colony morphology. Use of CDBA-F1 could provide a sensitive and selective media for detection of\n                      <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                      isolates with reduced susceptibility while substantially reducing labor and supply costs.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                        isolates with reduced fidaxomicin susceptibility may emerge during CDI therapy and, in some cases, result in treatment failure. We report that supplementation of\n                        <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                        selective media with fidaxomicin results in an effective, efficient, and reduced cost method to screen stool for isolates with reduced fidaxomicin susceptibility. Use of this approach could dramatically increase capacity to conduct surveillance for reduced fidaxomicin susceptibility in\n                        <jats:italic toggle=\"yes\">C. difficile<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.00068-26","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T13:00:20Z","timestamp":1781269220000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["A sensitive and reduced-cost culture medium for recovery of\n                    <i>Clostridioides difficile<\/i>\n                    isolates with reduced fidaxomicin susceptibility from stool"],"prefix":"10.1128","volume":"64","author":[{"given":"Claire E.","family":"Kaple","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051fd9666","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Cadnum","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dbx6743","id-type":"ROR","asserted-by":"publisher"}],"name":"Research Service, Louis Stokes Cleveland VA Medical Center","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah N.","family":"Redmond","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051fd9666","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, Case Western Reserve University School of Medicine","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette L.","family":"Jencson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dbx6743","id-type":"ROR","asserted-by":"publisher"}],"name":"Research Service, Louis Stokes Cleveland VA Medical Center","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6300-2280","authenticated-orcid":true,"given":"Munok","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Research, Central Texas Veterans Health Care System","place":["Temple, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8272-9828","authenticated-orcid":true,"given":"Hosoon","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Research, Central Texas Veterans Health Care System","place":["Temple, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3916-952X","authenticated-orcid":true,"given":"Chetan","family":"Jinadatha","sequence":"additional","affiliation":[{"name":"Department of Medicine, Central Texas Veterans Health Care System","place":["Temple, USA"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Education, College of Medicine, Texas A&M University","place":["Bryan, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0990-4498","authenticated-orcid":true,"given":"Curtis J.","family":"Donskey","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051fd9666","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, Case Western Reserve University School of Medicine","place":["Cleveland, USA"]},{"id":[{"id":"https:\/\/ror.org\/01vrybr67","id-type":"ROR","asserted-by":"publisher"}],"name":"Geriatric Research, Education and Clinical Center, Louis Stokes Cleveland VA Medical Center","place":["Cleveland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1910215"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab718"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac817"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00349-23"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00391-19"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03708-7"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03708-7"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01206-24"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaf028"},{"key":"e_1_3_5_11_2","volume-title":"Methods for antimicrobial susceptibility testing of anaerobic bacteria","author":"Clinical and Laboratory Standards Institute","year":"2018","unstructured":"Clinical and Laboratory Standards Institute. 2018. CLSI standard M11. In Methods for antimicrobial susceptibility testing of anaerobic bacteria, 9th ed. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"9"},{"key":"e_1_3_5_12_2","unstructured":"Prinzi A Rohde RE. 2020 Why differential & selective media remain invaluable tools. Available from: https:\/\/asm.org:443\/articles\/2020\/september\/why-differential-selective-media-are-invaluable-to"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01591-08"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00793-14"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/314874"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics4030267"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/su142416563"},{"key":"e_1_3_5_18_2","unstructured":"IFEU - Institut f\u00fcr Energie- und Umweltforschung. 2017. Eco-profile of polyolefins (HDPE and PP) in the Gulf Cooperation Council (GCC). Available from: https:\/\/www.ifeu.de\/en\/project\/oekoprofil-von-polyolefinen-aus-laendern-des-mittleren-ostens\/"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1013471"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2021.102418"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/mra.01128-23"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00130-26"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac444"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00068-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00068-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:01:08Z","timestamp":1783515668000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00068-26"}},"subtitle":[],"editor":[{"given":"Alexander","family":"Mellmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,7,8]]},"references-count":22,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7,8]]}},"alternative-id":["10.1128\/jcm.00068-26"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00068-26","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,8]]},"assertion":[{"value":"2026-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00068-26"}}