{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:49:56Z","timestamp":1762865396948},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Drug resistance in\n            <jats:italic>Plasmodium falciparum<\/jats:italic>\n            is a serious public health threat in the countries where this organism is endemic. Since resistance has been associated with specific single-nucleotide polymorphisms (SNPs) in parasite genes, molecular markers are becoming useful surrogates for monitoring the emergence and dispersion of drug resistance. In this study, a multiplex PCR (mPCR) and oligonucleotide microarray method was developed for the detection of these SNPs in genes encoding chloroquine resistance transporter (Pf\n            <jats:italic>crt<\/jats:italic>\n            ), multidrug resistance 1 (Pf\n            <jats:italic>mdr1<\/jats:italic>\n            ), dihydrofolate reductase (Pf\n            <jats:italic>dhfr<\/jats:italic>\n            ), dihydropteroate synthetase (Pf\n            <jats:italic>dhps<\/jats:italic>\n            ), and ATPase 6 (Pf\n            <jats:italic>ATPase6<\/jats:italic>\n            ) of\n            <jats:italic>P. falciparum<\/jats:italic>\n            . The results show that DNA microarray technology, combined with mPCR, is a promising and time-saving tool that supports conventional detection methods, allowing sensitive, accurate, simultaneous analysis of the SNPs associated with drug resistance in\n            <jats:italic>P. falciparum<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.00081-08","type":"journal-article","created":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T01:27:08Z","timestamp":1209605228000},"page":"2167-2174","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Multiplex PCR and Oligonucleotide Microarray for Detection of Single-Nucleotide Polymorphisms Associated with\n            <i>Plasmodium falciparum<\/i>\n            Drug Resistance"],"prefix":"10.1128","volume":"46","author":[{"given":"Guo Qing","family":"Zhang","sequence":"first","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis, and Filariasis, 207 Rui Jin Er Road, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya Yi","family":"Guan","sequence":"additional","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis, and Filariasis, 207 Rui Jin Er Road, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai Hui","family":"Sheng","sequence":"additional","affiliation":[{"name":"National Engineering Center for Biochip at Shanghai, 151 Li Bing Road, Pudong, Shanghai 201203, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating 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