{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:02:47Z","timestamp":1761580967557},"reference-count":20,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n            is the causative agent of syphilis, a sexually transmitted infection of significant public health importance. Since 2000 there has been a marked increase in the number of cases of syphilis infections notified in Victoria, Australia, with the majority of cases occurring in men who have sex with men (MSM) and the highest incidence being in HIV-infected MSM. The molecular subtyping method described by Pillay et al. (A. Pillay et al., Sex. Transm. Dis. 25:408\u2013414, 1998) has been used in this study to determine the diversity of\n            <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n            subtypes circulating locally and to look for any relationship between\n            <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n            subtypes and HIV status over a 6-year period (2004 to 2009).\n            <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n            DNA was detected in 303 patient specimens (\n            <jats:italic>n<\/jats:italic>\n            = 3,652), and full subtyping profiles were obtained from 90 of these (from 88 patients). A total of 11\n            <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n            subtypes were identified: types 14e (28, 31.1%), 14d (15, 16.7%), 14k (13, 14.4%), 14p (12, 13.3%), 14i (7, 7.8%) 14b (6, 6.7%), 14l (5, 5.6%), and 12i, 13b, 13i, and 13e (1 each, 1.1%). This study showed a similar level of variation among circulating\n            <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n            strains compared with that in other studies using the same methodology. A different mix of strains and different predominating strains have been found at each geographical study location, with type 14e emerging as the predominant local strain in Victoria. There was no detectable trend between\n            <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n            subtypes and the specimen collection site or stage of syphilis (where known), nor was there any relationship between particular strains and HIV status.\n          <\/jats:p>","DOI":"10.1128\/jcm.00083-12","type":"journal-article","created":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T01:00:51Z","timestamp":1331773251000},"page":"1895-1899","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Molecular Subtyping of Treponema pallidum during a Local Syphilis Epidemic in Men Who Have Sex with Men in Melbourne, Australia"],"prefix":"10.1128","volume":"50","author":[{"given":"Francesca","family":"Azzato","sequence":"first","affiliation":[{"name":"Microbiology Division, Victorian Infectious Diseases Reference Laboratory, North Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"Ryan","sequence":"additional","affiliation":[{"name":"Microbiology Division, Victorian Infectious Diseases Reference Laboratory, North Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet","family":"Fyfe","sequence":"additional","affiliation":[{"name":"Microbiology Division, Victorian Infectious Diseases Reference Laboratory, North Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"Leslie","sequence":"additional","affiliation":[{"name":"Microbiology Division, Victorian Infectious Diseases Reference Laboratory, North Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00287-08"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.036301"},{"key":"e_1_3_1_4_2","first-page":"38","article-title":"Human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS)","volume":"13","author":"El-Hayek C","year":"2010","unstructured":"El-HayekC FeiginA. 2010. Human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS). Victoria Infect. Dis. Bull. 13:38.","journal-title":"Victoria Infect. Dis. Bull."},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00128-08"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5375.375"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01186-07"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1071\/SH10095"},{"key":"e_1_3_1_9_2","first-page":"37","article-title":"Syphilis\u2014infectious","volume":"13","author":"Higgins N","year":"2010","unstructured":"HigginsN. 2010. Syphilis\u2014infectious. Victoria Infect. Dis. Bull. 13:37\u201338.","journal-title":"Victoria Infect. Dis. Bull."},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01578-06"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01061-8"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/656533"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/600878"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181d73ce1"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199809000-00004"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.1.256-258.2002"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.3743-3746.2005"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2005.015040"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.040857"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/320698"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-443-06839-3.00238-1"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00083-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00083-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:31:20Z","timestamp":1646472680000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00083-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1128\/JCM.00083-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00083-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6]]},"assertion":[{"value":"2012-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}