{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T02:56:15Z","timestamp":1648954575770},"reference-count":24,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In Catalonia, a screening protocol for cervical cancer, including human papillomavirus (HPV) DNA testing using the Digene Hybrid Capture 2 (HC2) assay, was implemented in 2006. In order to monitor interlaboratory reproducibility, a proficiency testing (PT) survey of the HPV samples was launched in 2008. The aim of this study was to explore the repeatability of the HC2 assay's performance. Participating laboratories provided 20 samples annually, 5 randomly chosen samples from each of the following relative light unit (RLU) intervals: &lt;0.5, 0.5 to 0.99, 1 to 9.99, and \u226510. Kappa statistics were used to determine the agreement levels between the original and the PT readings. The nature and origin of the discrepant results were calculated by bootstrapping. A total of 946 specimens were retested. The kappa values were 0.91 for positive\/negative categorical classification and 0.79 for the four RLU intervals studied. Sample retesting yielded systematically lower RLU values than the original test (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.005), independently of the time elapsed between the two determinations (median, 53 days), possibly due to freeze-thaw cycles. The probability for a sample to show clinically discrepant results upon retesting was a function of the RLU value; samples with RLU values in the 0.5 to 5 interval showed 10.80% probability to yield discrepant results (95% confidence interval [CI], 7.86 to 14.33) compared to 0.85% probability for samples outside this interval (95% CI, 0.17 to 1.69). Globally, the HC2 assay shows high interlaboratory concordance. We have identified differential confidence thresholds and suggested the guidelines for interlaboratory PT in the future, as analytical quality assessment of HPV DNA detection remains a central component of the screening program for cervical cancer prevention.\n          <\/jats:p>","DOI":"10.1128\/jcm.00100-14","type":"journal-article","created":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T05:11:14Z","timestamp":1393477874000},"page":"1511-1518","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Interlaboratory Reproducibility and Proficiency Testing within the Human Papillomavirus Cervical Cancer Screening Program in Catalonia, Spain"],"prefix":"10.1128","volume":"52","author":[{"given":"R.","family":"Ib\u00e1\u00f1ez","sequence":"first","affiliation":[{"name":"Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"F\u00e9lez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat, Barcelona, Spain"},{"name":"Infections and Cancer, Bellvitge Institute of Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"God\u00ednez","sequence":"additional","affiliation":[{"name":"Infections and Cancer, Bellvitge Institute of Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Guardi\u00e0","sequence":"additional","affiliation":[{"name":"Primary Care Clinical Laboratory Barcelon\u00e9s Nord y Vall\u00e9s Oriental, Catalan Institute of Health, Badalona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Caballero","sequence":"additional","affiliation":[{"name":"Microbiology Department, University Hospital Vall d'Hebron, Catalan Institute of Health, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Juve","sequence":"additional","affiliation":[{"name":"Primary Care Clinical Laboratory Bon Pastor, Catalan Institute of Health, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Combalia","sequence":"additional","affiliation":[{"name":"Pathology Department, UDIAT-CD, University Hospital Parc Tauli, Universitat Aut\u00f2noma de Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Bellosillo","sequence":"additional","affiliation":[{"name":"Pathology Department, Hospital del Mar, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Cuevas","sequence":"additional","affiliation":[{"name":"Pathology and Molecular Genetics Department, University Hospital Arnau de Vilanova, Catalan Institute of Health, Lleida, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Moreno-Crespi","sequence":"additional","affiliation":[{"name":"Pathology Department, University Hospital of Girona Dr. Josep Trueta, Infections and Cancer Unit, Catalan Institute of Oncology, Girona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Pons","sequence":"additional","affiliation":[{"name":"Pathology Department, IISPV, URV, Hospital de Tortosa Verge de la Cinta (HTVC), Catalan Institute of Health, Tortosa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Autonell","sequence":"additional","affiliation":[{"name":"Pathology Department, Hospital Consortium of Vic, Vic, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Clinical Laboratory ICS Tarragona, Molecular Biology Section, University Hospital of Tarragona Joan XXIII, Catalan Institute of Health, Tarragona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Ordi","sequence":"additional","affiliation":[{"name":"Pathology Department, CRESIB\u2013Hospital Cl\u00ednic, University of Barcelona, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"de Sanjos\u00e9","sequence":"additional","affiliation":[{"name":"Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat, Barcelona, Spain"},{"name":"Infections and Cancer, Bellvitge Institute of Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain"},{"name":"CIBER Epidemiology and Public Health, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. G.","family":"Bravo","sequence":"additional","affiliation":[{"name":"Infections and Cancer Unit, Cancer Epidemiology Research Program, Catalan Institute of Oncology (ICO), L'Hospitalet de Llobregat, Barcelona, Spain"},{"name":"Infections and Cancer, Bellvitge Institute of Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70230-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021641"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_3_3_5_2","volume-title":"Cervix cancer screening","author":"IARC Working Group on the Evaluation of Cancer Prevention Strategies","year":"2005","unstructured":"IARC Working Group on the Evaluation of Cancer Prevention Strategies. 2005. 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Addison-Wesley, Reading, United Kingdom."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11723"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1309\/BA43HMCAJ26VWQH3"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-07-2904"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02078-05"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2005.01.009"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00100-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00100-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:58:23Z","timestamp":1646481503000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00100-14"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1128\/JCM.00100-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00100-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5]]},"assertion":[{"value":"2014-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-02-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-04-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}