{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T06:59:40Z","timestamp":1768892380160,"version":"3.49.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T00:00:00Z","timestamp":1251763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The objective of this study was to characterize by serotyping, pulsed-field gel electrophoresis (PFGE), and PCR amplification of virulence genes and markers of epidemic clones I, II, and III (ECI, ECII, and ECIII) 54 human isolates from apparently sporadic cases of infection occurring in the Lombardy region and in the province of Florence, Tuscany, Italy, in the years 1996 to 2007.\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            isolates were provided by the clinical microbiology laboratories of the Lombardy region and the \u201cCareggi\u201d Hospital of Florence, Tuscany, Italy. Serotyping, PFGE after digestion with the AscI and ApaI enzymes, and PCR amplification for the\n            <jats:italic>inlA<\/jats:italic>\n            ,\n            <jats:italic>inlC<\/jats:italic>\n            , and\n            <jats:italic>inlJ<\/jats:italic>\n            genes and ECI, ECII, and ECIII markers were performed according to procedures described previously. Twenty-five (46.3%)\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            isolates were assigned to serotype 1\/2a, 23 (42.6%) to serotype 4b, and 6 (11.1%) to serotype 1\/2b. Thirty-one AscI pulsotypes were recognized among the 54 human isolates. Eleven molecular subtype clusters, of which eight included indistinguishable pulsotypes and three included closely related pulsotypes, were shared by two to seven isolates. Fifteen isolates exhibited unique AscI pulsotypes. Three groups of clustered isolates and two apparently sporadic isolates generated EC amplicons. All strains tested positive for the\n            <jats:italic>inlA<\/jats:italic>\n            ,\n            <jats:italic>inlC<\/jats:italic>\n            , and\n            <jats:italic>inlJ<\/jats:italic>\n            genes. Based on the results of serotyping and molecular typing, there were 11 occasions when\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            strains with the same subtype were isolated from more than one listeriosis case. A total of 39 out of 54 isolates (72.2%) were attributed to molecular subtype clusters. The results of the study suggest that routine subtyping of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            strains from human listeriosis cases could allow more-timely detection of outbreaks possibly caused by food-borne isolates from a common source and could lead to control of ongoing food exposure, thus preventing the occurrence of more cases.\n          <\/jats:p>","DOI":"10.1128\/jcm.00102-09","type":"journal-article","created":{"date-parts":[[2009,7,16]],"date-time":"2009-07-16T01:44:18Z","timestamp":1247708658000},"page":"2925-2930","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Characterization of\n            <i>Listeria monocytogenes<\/i>\n            Isolates from Human Listeriosis Cases in Italy"],"prefix":"10.1128","volume":"47","author":[{"given":"Caterina","family":"Mammina","sequence":"first","affiliation":[{"name":"Department of Sciences for Health Promotion G. D'Alessandro, University of Palermo, Palermo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aurora","family":"Aleo","sequence":"additional","affiliation":[{"name":"Department of Sciences for Health Promotion G. D'Alessandro, University of Palermo, Palermo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Romani","sequence":"additional","affiliation":[{"name":"Department of Public Health, University of Florence"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Pellissier","sequence":"additional","affiliation":[{"name":"Department of Public Health, Microbiology and Virology, University of Milan, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierluigi","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Virology, Azienda Ospedaliero-Universitaria Careggi, Florence"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizia","family":"Pecile","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Virology, Azienda Ospedaliero-Universitaria Careggi, Florence"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonino","family":"Nastasi","sequence":"additional","affiliation":[{"name":"Department of Public Health, University of Florence"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirella M.","family":"Pontello","sequence":"additional","affiliation":[{"name":"Department of Public Health, Microbiology and Virology, University of Milan, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00961-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01873-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.5291-5294.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-70.5.1116"},{"key":"e_1_3_2_7_2","first-page":"pii=8082","volume":"13","year":"2008","unstructured":"Denny, J., and J. McLauchlin. 2008. Human Listeria monocytogenes infections in Europe\u2014an opportunity for improved European surveillance. Euro Surveill.13(13):pii=8082. http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId=8082 .","journal-title":"Euro Surveill."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/154.10.944"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00404.x"},{"key":"e_1_3_2_10_2","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ). 2007. Request for updating the former SCVPH opinion on Listeria monocytogenes risk related to ready-to-eat foods and scientific advice on different levels of Listeria monocytogenes in ready-to-eat foods and the related risk for human illness. Scientific Opinion of the Panel on Biological Hazards. http:\/\/www.efsa.europa.eu\/EFSA\/efsa_locale-1178620753812_1178680093176.htm\n."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-70.3.574"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.12.2929"},{"key":"e_1_3_2_13_2","first-page":"E7","volume":"12","year":"2007","unstructured":"Gianfranceschi, M., A. Gattuso, M. D'Ottavio, S. Fokas, and P. Aureli. 2007. Results of a 12-month long enhanced surveillance of listeriosis in Italy. 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