{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:51:55Z","timestamp":1792741915116,"version":"4.5.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacterial vaginosis (BV) is a recurrent condition that is associated with a range of negative outcomes, including the acquisition of human immunodeficiency virus and other sexually transmitted diseases, preterm births, and pelvic inflammatory disease. In contrast to the\n            <jats:italic>Lactobacillus<\/jats:italic>\n            -dominated normal vaginal microbiota, BV is characterized by a lack of lactobacilli and an abundance of anaerobic and gram-negative organisms, including\n            <jats:italic>Gardnerella vaginalis<\/jats:italic>\n            and\n            <jats:italic>Atopobium vaginae<\/jats:italic>\n            . To date, the laboratory diagnosis of BV has relied upon the fulfillment of criteria determined by microscopic observation of Gram-stained vaginal swabs. We describe a molecular-based method for the easy determination of the species profile within the vaginal microbiota based on the amplification of the chaperonin-60 genes of all bacteria present in the swab and hybridization of the amplicon to species-specific oligonucleotide-coupled fluorescent beads that are identified by flow cytometry with a Luminex instrument. We designed a nineplex Luminex array for characterization of the vaginal microbiota and applied it to the analysis of vaginal swabs from individuals from Africa and North America. Using the presence of\n            <jats:italic>A. vaginae<\/jats:italic>\n            or\n            <jats:italic>G. vaginalis<\/jats:italic>\n            , or both, as the defining criterion for BV, we found that the method was highly specific and sensitive for the diagnosis of BV using microscopy as a gold standard.\n          <\/jats:p>","DOI":"10.1128\/jcm.00112-09","type":"journal-article","created":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T02:09:13Z","timestamp":1254362953000},"page":"4067-4077","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Multiplex Detection of Bacteria Associated with Normal Microbiota and with Bacterial Vaginosis in Vaginal Swabs by Use of Oligonucleotide-Coupled Fluorescent Microspheres"],"prefix":"10.1128","volume":"47","author":[{"given":"Tim J.","family":"Dumonceaux","sequence":"first","affiliation":[{"name":"National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, MB, Canada R3K 0R2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Schellenberg","sequence":"additional","affiliation":[{"name":"Departments of Medical Microbiology and Immunology, University of Manitoba, 730 William Avenue, Winnipeg, MB, Canada R3E 0W3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Goleski","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, MB, Canada R3K 0R2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet E.","family":"Hill","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK, Canada S7N 5B4"},{"name":"Women's Health Research Institute, Provincial Health Services Authority, B325B, 4500 Oak Street, Vancouver, BC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter","family":"Jaoko","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Nairobi, P.O. 19676, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Kimani","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, MB, Canada R3K 0R2"},{"name":"Department of Medical Microbiology, University of Nairobi, P.O. 19676, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Money","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of British Columbia, 2H30, 4500 Oak Street, Vancouver, BC, Canada"},{"name":"Women's Health Research Institute, Provincial Health Services Authority, B325B, 4500 Oak Street, Vancouver, BC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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