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Sequence variation was assessed in regions within the small subunit of nuclear rRNA (p-SSU), the 70-kDa heat shock protein (p-<jats:italic>hsp70<\/jats:italic>), and the 60-kDa glycoprotein (p-<jats:italic>gp60<\/jats:italic>) genes by employing single-strand conformation polymorphism analysis and sequencing. Based on the analyses of p-SSU and p-<jats:italic>hsp70<\/jats:italic>,<jats:italic>Cryptosporidium hominis<\/jats:italic>(<jats:italic>n<\/jats:italic>= 38) and<jats:italic>Cryptosporidium parvum<\/jats:italic>(<jats:italic>n<\/jats:italic>= 24) were identified. The analysis of p-<jats:italic>gp60<\/jats:italic>revealed eight distinct subgenotypes, classified as<jats:italic>C. hominis<\/jats:italic>IaA17R1 (<jats:italic>n<\/jats:italic>= 3), IbA9G3R2 (<jats:italic>n<\/jats:italic>= 14), IbA10G2R2 (<jats:italic>n<\/jats:italic>= 20), and IfA12G1R1 (<jats:italic>n<\/jats:italic>= 1), as well as<jats:italic>C. parvum<\/jats:italic>IIaA18G3R1 (<jats:italic>n<\/jats:italic>= 15), IIaA20G3R1 (<jats:italic>n<\/jats:italic>= 6), IIaA22G4R1 (<jats:italic>n<\/jats:italic>= 2), and IIcA5G3R2 (<jats:italic>n<\/jats:italic>= 1). Subgenotypes IaA17R1 and IIaA22G4R1 are new. Of the six other subgenotypes, IbA10G2R2, IIaA18G3R1, IIaA20G3R1, and IIcA5G3R2 were reported previously from the state of Victoria. This is the fourth record in Australia of<jats:italic>C. parvum<\/jats:italic>subgenotype IIaA18G3R1 from humans, which, to date, has been isolated only from cattle in other countries. This subgenotype might be a significant contributor to sporadic human cryptosporidiosis and may indicate a greater zoonotic contribution to the infection of humans in the area of study. Comparative analyses revealed, for the first time, the differences in the genetic makeup of<jats:italic>Cryptosporidium<\/jats:italic>populations between two relatively close, major metropolitan cities.<\/jats:p>","DOI":"10.1128\/jcm.00116-08","type":"journal-article","created":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T01:27:08Z","timestamp":1209605228000},"page":"2252-2262","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Classification of<i>Cryptosporidium<\/i>Species from Patients with Sporadic Cryptosporidiosis by Use of Sequence-Based Multilocus Analysis following Mutation Scanning"],"prefix":"10.1128","volume":"46","author":[{"given":"Aaron R.","family":"Jex","sequence":"first","affiliation":[{"name":"Department of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aradhana","family":"Pangasa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bronwyn E.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Whipp","sequence":"additional","affiliation":[{"name":"Melbourne Diagnostic Unit, Victoria 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoff","family":"Hogg","sequence":"additional","affiliation":[{"name":"Melbourne Diagnostic Unit, Victoria 3052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha I.","family":"Sinclair","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Preventive Medicine, Monash University, Central and Eastern Clinical School, Alfred Hospital, Melbourne, Victoria 3004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melita","family":"Stevens","sequence":"additional","affiliation":[{"name":"Melbourne Water Corporation, East Melbourne, Victoria 3002, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, The University of Melbourne, Werribee, Victoria 3030, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0140-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094786"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-8508(03)00030-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-843R.1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2006.00162.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0164-5"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2744-2747.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767130-00015"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.1.2.297"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki456"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1071\/NB06018"},{"key":"e_1_3_2_13_2","first-page":"185","volume":"47","year":"2005","unstructured":"Cacci\u00f2, S. 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