{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:45:59Z","timestamp":1792572359260,"version":"4.5.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T00:00:00Z","timestamp":1243814400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The aim of this study was to develop and evaluate a sensitive method for the simultaneous identification of 14 urogenital potential pathogens. A multiplex PCR-based reverse line blot (mPCR\/RLB) assay was developed to detect 14 urogenital pathogens or putative pathogens, namely\n            <jats:italic>Trichomonas vaginalis<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            ,\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            ,\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            ,\n            <jats:italic>Ureaplasma parvum<\/jats:italic>\n            ,\n            <jats:italic>U. urealyticum<\/jats:italic>\n            ,\n            <jats:italic>Gardnerella vaginalis<\/jats:italic>\n            ,\n            <jats:italic>Haemophilus influenzae<\/jats:italic>\n            , herpes simplex virus type 1 (HSV1) and HSV2,\n            <jats:italic>N. meningitidis<\/jats:italic>\n            ,\n            <jats:italic>Mycoplasma hominis<\/jats:italic>\n            ,\n            <jats:italic>M. genitalium<\/jats:italic>\n            , and adenovirus, using two species-specific primer pairs and probes for each. The method was validated using a reference strain or a well-characterized clinical isolate of each target organism and was found to be both sensitive and specific. The limits of detection for the mPCR\/RLB assay varied among the 14 target organisms from 4.2 \u00d7 10\n            <jats:sup>\u22121<\/jats:sup>\n            to 7.0 \u00d7 10\n            <jats:sup>\u221211<\/jats:sup>\n            ng\/\u03bcl of genomic DNA. There were no cross-reactions among any of the probes. This method was used to test 529 first-voided urine specimens from male patients with and without urethritis attending two Sydney sexual health clinics. One or more target species were detected in 193 (36%) subjects. Of 233 positive results, overall 216 (93%) were concordant between mPCR\/RLB and a comparator method (culture and\/or species-specific PCR), 9 were positive only by mPCR\/RLB, and 8 were positive only by the comparator method. The mPCR\/RLB method was an accurate, convenient, and inexpensive method for the detection of multiple potential pathogens in first-voided urine specimens from men.\n          <\/jats:p>","DOI":"10.1128\/jcm.00120-09","type":"journal-article","created":{"date-parts":[[2009,4,9]],"date-time":"2009-04-09T01:07:01Z","timestamp":1239239221000},"page":"1871-1877","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR-Based Reverse Line Blot Assay"],"prefix":"10.1128","volume":"47","author":[{"given":"Michelle L.","family":"Mckechnie","sequence":"first","affiliation":[{"name":"Sexually Transmitted Infections Research Centre (STIRC), University of Sydney, Marian Villa, Westmead Hospital, New South Wales 2145, Australia"},{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Hillman","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Infections Research Centre (STIRC), University of Sydney, Marian Villa, Westmead Hospital, New South Wales 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Couldwell","sequence":"additional","affiliation":[{"name":"Parramatta Sexual Health Clinic, Level 2, Jeffery House, 158 Marsden St., Parramatta, New South Wales 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanrong","family":"Kong","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanor","family":"Freedman","sequence":"additional","affiliation":[{"name":"Sydney Sexual Health Centre, Sydney Hospital and Eye Hospital, Macquarie Street, Sydney, New South Wales 2000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales 2145, Australia"},{"name":"Research Laboratory for Infectious Skin Diseases, Department of Dermatology, Wuhan First Hospital, Wuhan 430022, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendolyn L.","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.498-505.2001"},{"key":"e_1_3_2_3_2","first-page":"1","volume":"35","year":"2004","unstructured":"Baczynska, A., H. F. Svenstrup, J. Fedder, S. Birkelund, and G. Christiansen. 2004. Development of real-time PCR for detection of Mycoplasma hominis. BMC Microbiol.35:1-13.","journal-title":"BMC Microbiol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2003.007641"},{"key":"e_1_3_2_5_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/095646240001100504"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.2002.tb04554.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/499434"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1071\/SH04028"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.778-782.2003"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.OLQ.0000114653.26951.71"},{"key":"e_1_3_2_12_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2004.010439"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199110000-00006"},{"key":"e_1_3_2_15_2","first-page":"5619","volume":"12","year":"2003","unstructured":"Hardick, J., S. Yang, S. Lin, D. Duncan, and C. Gaydos. 2003. Use of the Roche LightCycler instrument in a real-time PCR for Trichomonas vaginalis in urine samples from females and males. J. Clin. Microbiol.12:5619-5622.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-198807000-00007"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45847-0"},{"key":"e_1_3_2_19_2","first-page":"373","volume":"73","year":"1997","unstructured":"Keane, F. E. A., B. J. Thomas, L. Whitaker, A. Renton, and D. Taylor-Robinson. 1997. An association between non-gonococcal urethritis and bacterial vaginosis and the implications for patients and their sexual partners. Genitourin. Med.73:373-377.","journal-title":"Genitourin. Med."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.404"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31815b0168"},{"key":"e_1_3_2_22_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_23_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_24_2","first-page":"13","volume":"60","year":"2005","unstructured":"Mallard, K., K. Schopfer, and T. Bodmer. 2005. Development of real-time PCR for the differential detection and quantification of Ureaplasma urealyticum and Ureaplasma parvum. J. Clin. Microbiol.60:13-19.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.2005.074062"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2006.01660.x"},{"key":"e_1_3_2_27_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_28_2","unstructured":"Manual of clinical microbiology 2007"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2004.01.013"},{"key":"e_1_3_2_30_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_31_2","first-page":"345","volume":"61","year":"1985","unstructured":"Noble, R. C. 1985. Colonisation of the urethra with Streptococcus pneumoniae: a case report. Genitourin. Med.61:345-346.","journal-title":"Genitourin. Med."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/131.4.376"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-001-0665-1"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.10.2286-2294.1989"},{"key":"e_1_3_2_35_2","first-page":"910","volume":"12","year":"2005","unstructured":"Rauter, C., M. Mueller, I. Diterich, S. Zeller, D. Hassler, T. Meergans, and T. Hartung. 2005. Critical evaluation of urine-based PCR assay for diagnosis of Lyme borreliosis. Clin. Diagn. Lab. Immun.12:910-917.","journal-title":"Clin. Diagn. Lab. Immun."},{"key":"e_1_3_2_36_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.9.3963-3969.2004"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199605233342103"},{"key":"e_1_3_2_39_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_40_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1528-1533.2004"},{"key":"e_1_3_2_42_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/153.1.165"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2007.01.011"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198005013021805"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.3.1483-1486.2004"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01890.x"},{"key":"e_1_3_2_48_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1413-1418.2006"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199110103251507"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000243621.89212.40"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2002.tb00634.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00120-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00120-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:02:15Z","timestamp":1646463735000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00120-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1128\/JCM.00120-09"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00120-09","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]},"assertion":[{"value":"2009-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-03-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}