{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:17:00Z","timestamp":1791346620176,"version":"4.3.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Ocular toxoplasmosis is a major cause of posterior uveitis worldwide. The diagnosis is based mainly on ophthalmological examination. Biological diagnosis is necessary in atypical cases, and this requires aqueous humor sampling by anterior chamber paracentesis. We evaluated real-time PCR targeting the\n            <jats:italic>Toxoplasma gondii<\/jats:italic>\n            529-bp repeat element, the Goldmann-Witmer coefficient (GWC), and immunoblotting for the diagnosis of toxoplasmic retinochoroiditis in 54 patients with atypical uveitis. The results of these biological tests, applied to paired aqueous humor-serum samples, were compared to the clinical findings. Combining either PCR or the GWC with immunoblotting increased the sensitivity to 73% or 70%, respectively. Together, PCR and the GWC had 80% sensitivity. If feasible, sensitivity can be increased by combining the three methods (85% sensitivity). The interval between symptom onset and anterior chamber paracentesis strongly influenced the detection of specific intraocular antibody synthesis. The sensitivity of the GWC increased from 45% to 56% when sampling was performed 10 days after symptom onset, and that of immunoblotting increased from 53% to 72% when puncture was performed 30 days after symptom onset. PCR analysis of aqueous humor samples detected toxoplasmic DNA in 55% of patients. In contrast to the results of immunoblotting and the GWC, the results of PCR were not influenced by the interval between symptom onset and paracentesis. PCR was more informative than the GWC and immunoblotting for immunocompromised patients. Acute necrotizing retinal lesions were significantly larger in PCR-positive patients, with a mean of 3.5 optic disc diameters, than in PCR-negative patients, with a mean of 1.5 optic disc diameters.\n          <\/jats:p>","DOI":"10.1128\/jcm.00128-09","type":"journal-article","created":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T00:45:14Z","timestamp":1242261914000},"page":"2131-2135","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Contributions of Immunoblotting, Real-Time PCR, and the Goldmann-Witmer Coefficient to Diagnosis of Atypical Toxoplasmic Retinochoroiditis"],"prefix":"10.1128","volume":"47","author":[{"given":"H.","family":"Talabani","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology-Mycology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Asseraf","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ho\u0302pital Cochin, AP-HP, Universite\u0301 Paris Descartes"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Yera","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology-Mycology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Delair","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ho\u0302pital Cochin, AP-HP, Universite\u0301 Paris Descartes"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Ancelle","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology-Mycology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Thulliez","sequence":"additional","affiliation":[{"name":"Laboratory of Toxoplasmosis, Institut de Pue\u0301riculture de Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P.","family":"Bre\u0301zin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ho\u0302pital Cochin, AP-HP, Universite\u0301 Paris Descartes"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Dupouy-Camet","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology-Mycology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"699","volume":"324","year":"1991","unstructured":"Brezin, A. P., C. E. Eqwuagu, C. Silveira, P. Thulliez, M. C. Martins, R. M. Mahdi, R. Belfort, Jr., and R. B. Nussenblatt. 1991. Analysis of aqueous humor in ocular toxoplasmosis. N. Engl. J. Med.324:699.","journal-title":"N. Engl. J. 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Ridings. 1995. Ocular toxoplasmosis: value of immunoblotting for the determination of an intra-ocular synthesis of antibodies. Pathol. Biol. (Paris)43:772-778. (In French.)","journal-title":"Pathol. Biol. 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