{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:40:41Z","timestamp":1791614441308,"version":"4.3.4"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We aimed to compare conventional<jats:italic>ompA<\/jats:italic>typing of<jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>with multilocus sequence typing (MLST) and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA). Previously used MLST and MLVA systems were compared to modified versions that used shorter target regions and nested PCR. Heterosexual couples were selected from among persons with urogenital<jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>infections visiting the sexually transmitted infection outpatient clinic in Amsterdam, The Netherlands. We identified 30 couples with a total of 65<jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>-positive samples on which MLST and MLVA for eight target regions were performed. All regions were successfully sequenced in 52 samples, resulting in a complete profile for 18 couples and 12 individuals. Nine<jats:italic>ompA<\/jats:italic>genovars from D to K, with two variants of genovar G, were found. The numbers of sequence type and MLVA type profiles were 20 for MLST and 21 for MLVA, and a combination of MLST and MLVA yielded 28 profiles, with discriminatory indexes (<jats:italic>D<\/jats:italic>) ranging from 0.95 to 0.99. Partners in 17 couples shared identical profiles, while partners in 1 couple had completely different profiles. Three persons had infections at multiple anatomical locations, and within each of these three individuals, all profiles were identical. The discriminatory capacity of all MLST and MLVA methods is much higher than that of<jats:italic>ompA<\/jats:italic>genotyping (<jats:italic>D<\/jats:italic>= 0.78). No genotype variation was found within the samples of the same person or from heterosexual couples with a putative single transmission. This shows that the chlamydial genome in clinical specimens has an appropriate polymorphism to enable epidemiological cluster analysis using MLST and MLVA.<\/jats:p>","DOI":"10.1128\/jcm.00128-11","type":"journal-article","created":{"date-parts":[[2011,6,9]],"date-time":"2011-06-09T15:13:42Z","timestamp":1307632422000},"page":"2844-2853","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":79,"title":["Evaluation of High-Resolution Typing Methods for Chlamydia trachomatis in Samples from Heterosexual Couples"],"prefix":"10.1128","volume":"49","author":[{"given":"Reinier J. M.","family":"Bom","sequence":"first","affiliation":[{"name":"Public Health Laboratory, Public Health Service of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linus","family":"Christerson","sequence":"additional","affiliation":[{"name":"Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten F.","family":"Schim van der Loeff","sequence":"additional","affiliation":[{"name":"Department of Research, Public Health Service of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roel A.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Centre of Infectious Diseases and Immunology Amsterdam, Academic Medical Centre, Amsterdam, The Netherlands"},{"name":"Centre for Infectious Disease Control, National Institute of Public Health and the Environment, Bilthoven, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia M.","family":"Bruisten","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Public Health Service of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/156.6.953"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.3201\/eid1611.100379","article-title":"Typing of lymphogranuloma venereum Chlamydia trachomatis strains. 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Infect. 13(Suppl. 3):1\u201346.","journal-title":"Infect."},{"key":"e_1_3_3_26_2","unstructured":"World Health Organization. 2010. Sexually transmitted diseases. 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