{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:05:11Z","timestamp":1791119111179,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            As an issue of biosecurity, species-specific genetic markers have been well characterized. However,\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            strain-specific information is currently not sufficient for traceability to identify the origin of the strain. By using genome-wide screening using short read mapping, we identified strain-specific single nucleotide polymorphisms (SNPs) among\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains including Japanese isolates, and we further developed a simplified 80-tag SNP typing method for the primary investigation of traceability. These 80-tag SNPs were selected from 2,965 SNPs on the chromosome and the pXO1 and pXO2 plasmids from a total of 19\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains, including the available genome sequences of 17 strains in the GenBank database and 2 Japanese isolates that were sequenced in this study. Phylogenetic analysis based on 80-tag SNP typing showed a higher resolution power to discriminate 12 Japanese isolates rather than the 25 loci identified by multiple-locus variable-number tandem-repeat analysis (MLVA). In addition, the 80-tag PCR testing enabled the discrimination of\n            <jats:italic>B. anthracis<\/jats:italic>\n            from other\n            <jats:italic>B. cereus<\/jats:italic>\n            group species, helping to identify whether a suspected sample originates from the intentional release of a bioterrorism agent or environmental contamination with a virulent agent. In conclusion, 80-tag SNP typing can be a rapid and sufficient test for the primary investigation of strain origin. Subsequent whole-genome sequencing will reveal apparent strain-specific genetic markers for traceability of strains following an anthrax outbreak.\n          <\/jats:p>","DOI":"10.1128\/jcm.00137-10","type":"journal-article","created":{"date-parts":[[2010,6,17]],"date-time":"2010-06-17T01:56:56Z","timestamp":1276739816000},"page":"2821-2829","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Genome-Wide Single Nucleotide Polymorphism Typing Method for Identification of\n            <i>Bacillus anthracis<\/i>\n            Species and Strains among\n            <i>B. cereus<\/i>\n            Group Species"],"prefix":"10.1128","volume":"48","author":[{"given":"Makoto","family":"Kuroda","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Genomics, Pathogen Genomics Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakuni","family":"Serizawa","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Genomics, Pathogen Genomics Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Okutani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Sekizuka","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Genomics, Pathogen Genomics Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satomi","family":"Banno","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Genomics, Pathogen Genomics Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp255"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00940-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00241-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-21"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.9.3382-3390.2006"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402414101"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.17.2236"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01582"},{"key":"e_1_3_2_10_2","first-page":"522","volume":"46","year":"2005","unstructured":"Jones, S. W., M. E. Dobson, S. C. Francesconi, R. Schoske, and R. Crawford. 2005. DNA assays for detection, identification, and individualization of select agent microorganisms. Croat. Med. J. 46 : 522-529.","journal-title":"Croat. Med. 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