{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:18:48Z","timestamp":1791217128127,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Haemophilus influenzae<\/jats:named-content>\n            type b (Hib) is, in contrast to non-type b\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            , associated with severe invasive disease, such as meningitis and epiglottitis, in small children. To date, accurate\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            capsule typing requires PCR, a time-consuming and cumbersome method. Matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS) provides rapid bacterial diagnostics and is increasingly used in clinical microbiology laboratories. Here, MALDI-TOF MS was evaluated as a novel approach to separate Hib from other\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            . PCR-verified Hib and non-Hib reference isolates were selected based on genetic and spectral characteristics. Mass spectra of reference isolates were acquired and used to generate different classification algorithms for Hib\/non-Hib differentiation using both ClinProTools and the MALDI Biotyper software. A test series of mass spectra from 33 Hib and 77 non-Hib isolates, all characterized by PCR, was used to evaluate the algorithms. Several algorithms yielded good results, but the two best were a ClinProTools model based on 22 separating peaks and subtyping main spectra (MSPs) using MALDI Biotyper. The ClinProTools model had a sensitivity of 100% and a specificity of 99%, and the results were 98% reproducible using a different MALDI-TOF MS instrument. The Biotyper subtyping MSPs had a sensitivity of 97%, a specificity of 100%, and 93% reproducibility. Our results suggest that it is possible to use MALDI-TOF MS to differentiate Hib from other\n            <jats:named-content content-type=\"genus-species\">H. influenzae<\/jats:named-content>\n            . This is a promising method for rapidly identifying Hib in unvaccinated populations and for the screening and surveillance of Hib carriage in vaccinated populations.\n          <\/jats:p>","DOI":"10.1128\/jcm.00137-15","type":"journal-article","created":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T02:21:46Z","timestamp":1430360506000},"page":"2215-2224","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Identification of Haemophilus influenzae Type b Isolates by Use of Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry"],"prefix":"10.1128","volume":"53","author":[{"given":"Viktor","family":"M\u00e5nsson","sequence":"first","affiliation":[{"name":"Clinical Microbiology, Department of Translational Medicine, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrik","family":"Resman","sequence":"additional","affiliation":[{"name":"Clinical Microbiology, Department of Translational Medicine, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Kostrzewa","sequence":"additional","affiliation":[{"name":"Bruker Daltonics, Bremen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Nilson","sequence":"additional","affiliation":[{"name":"Clinical Microbiology, Labmedicin, Region Sk\u00e5ne, Sweden"},{"name":"Division of Medical Microbiology, Department of Laboratory Medicine, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Riesbeck","sequence":"additional","affiliation":[{"name":"Clinical Microbiology, Department of Translational Medicine, Lund University, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.302-317.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000056"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.07.100"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-1994-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03417.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-35"},{"key":"e_1_3_2_8_2","unstructured":"Lepp T Uhnoo I Sch\u00f6lin A Szirmai M Lindstrand A Tegnell A. 2014. The childhood immunization program in Sweden 2013 annual report. Public Health Agency of Sweden Solna Sweden. http:\/\/www.skane.se\/Upload\/Webbplatser\/Smittskydd\/Dokument\/vaccinationst%C3%A4ckning\/A%CC%8Arsrapport_barnvaccinationsprogrammet%202013%5B2%5D.pdf."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61203-4"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02509-10"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.10.2382-2386.1994"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.07.004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00794-07"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.20.7758"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.8.1837-1845.1988"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01207-07"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03311.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03368.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-013-0609-x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01872-14"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101924"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12715"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1623-1636.2003"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01526-13"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12113"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01265-14"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2741-2749.2005"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-1958-x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00626-12"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00137-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00137-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:12:46Z","timestamp":1646482366000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00137-15"}},"subtitle":[],"editor":[{"given":"A. 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