{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:52:45Z","timestamp":1790970765751,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n We aimed to detect the etiological agents of acute diarrhea by a molecular gastrointestinal pathogen test (MGPT) and to assess the impact of MGPT on antimicrobial stewardship programs (ASP). This is a prospective observational study and was conducted between 1 January 2015 and 30 June 2017. We included consequent patients who had acute diarrhea. At the end of 2015, we implemented ASP in acute diarrhea cases and compared the outcomes in the pre-ASP and post-ASP periods. An FDA-cleared multiplexed gastrointestinal PCR panel system, the BioFire FilmArray (Idaho Technology, Salt Lake City, UT), which detects 20 pathogens in stool, was used. In 499 out of 699 patients (71%), at least one pathogen was detected. Among 314 adults with positive MGPT, 101 (32%) enteropathogenic\n Escherichia coli<\/jats:named-content>\n (EPEC), 71 (23%) enteroaggregative\n E. coli<\/jats:named-content>\n (EAEC), 68 (22%) enterotoxigenic\n E. coli<\/jats:named-content>\n (ETEC), 55 (18%) Shiga toxin-producing\n E. coli<\/jats:named-content>\n (STEC) (17%)\n Norovirus<\/jats:named-content>\n , 48 (15%)\n Campylobacter<\/jats:named-content>\n , 21 (7%)\n Salmonella<\/jats:named-content>\n , and 20 (6%)\n Clostridium difficile<\/jats:named-content>\n strains were detected. Among 185 children, 55 (30%) EPEC, 37 (20%)\n C. difficile<\/jats:named-content>\n , 32 (17%)\n Norovirus<\/jats:named-content>\n , 29 (16%) EAEC, 22 (12%) STEC, 21 (11%) ETEC, 21 (11%)\n Campylobacter<\/jats:named-content>\n , 20 (11%)\n Salmonella<\/jats:named-content>\n , and 16 (5%)\n Rotavirus<\/jats:named-content>\n strains were detected. Inappropriate antibiotic use decreased in the post-ASP period compared with the pre-ASP period among inpatients (42.9% and 25.8%, respectively;\n P<\/jats:italic>\n = 0.023). Using MGPT in clinical practice significantly decreased the unnecessary use of antibiotics. Detection of high rates of\n C. difficile<\/jats:named-content>\n in children and\n Salmonella<\/jats:named-content>\n spp., as well as relatively high rates of\n Campylobacter<\/jats:named-content>\n spp., which were hard to isolate by routine stool culture, were remarkable.\n <\/jats:p>","DOI":"10.1128\/jcm.00148-18","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T10:10:27Z","timestamp":1520331027000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Rapid Molecular Detection of Gastrointestinal Pathogens and Its Role in Antimicrobial Stewardship"],"prefix":"10.1128","volume":"56","author":[{"given":"\u015eiran","family":"Keske","sequence":"first","affiliation":[{"name":"Infectious Diseases and Clinical Microbiology Department, American Hospital, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burak","family":"Zabun","sequence":"additional","affiliation":[{"name":"School of Medicine, Ko\u00e7 University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kahraman","family":"Aksoy","sequence":"additional","affiliation":[{"name":"School of Medicine, Ko\u00e7 University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00fcsun","family":"Can","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Department, School of Medicine, Ko\u00e7 University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erhan","family":"Palao\u011flu","sequence":"additional","affiliation":[{"name":"Central Laboratory, American Hospital, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6nder","family":"Erg\u00f6n\u00fcl","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Clinical Microbiology Department, School of Medicine, Ko\u00e7 University, Istanbul, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2016.126"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000768"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268816002533"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-017-1018-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.04.007"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01637-14"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix288"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.08.001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv252"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-017-3174-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix669"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6615a1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61602-X"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(99)00128-X"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8553(05)70206-5"},{"key":"e_1_3_2_17_2","unstructured":"Centers for Disease Control and Prevention. 2013. Antibiotic resistance threats in the United States 2013. Centers for Disease Control and Prevention Atlanta GA. https:\/\/www.cdc.gov\/drugresistance\/threat-report-2013\/."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30325-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku046"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2017.06.022"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00148-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00148-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:59:17Z","timestamp":1646485157000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00148-18"}},"subtitle":[],"editor":[{"given":"Andrew B.","family":"Onderdonk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,5]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1128\/JCM.00148-18"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00148-18","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5]]},"assertion":[{"value":"2018-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00148-18"}}