{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:16:09Z","timestamp":1787062569007,"version":"build-2736575974"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Brucellosis is a highly zoonotic disease that affects animals and human beings.\n                    <jats:named-content content-type=\"genus-species\">Brucella suis<\/jats:named-content>\n                    is the etiological agent of porcine brucellosis and one of the major human brucellosis pathogens. Laboratory diagnosis of porcine brucellosis mainly relies on serological tests, and it has been widely demonstrated that serological assays based on the detection of anti O-polysaccharide antibodies are the most sensitive tests. Here, we validate a recombinant glycoprotein antigen, an\n                    <jats:italic>N<\/jats:italic>\n                    -formylperosamine O-polysaccharide\u2013protein conjugate (OAg-AcrA), for diagnosis of porcine brucellosis. An indirect immunoassay based on the detection of anti-O-polysaccharide IgG antibodies was developed coupling OAg-AcrA to enzyme-linked immunosorbent assay plates (glyco-iELISA). To validate the assay, 563 serum samples obtained from experimentally infected and immunized pigs, as well as animals naturally infected with\n                    <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n                    biovar 1 or 2, were tested. A receiver operating characteristic (ROC) analysis was performed, and based on this analysis, the optimum cutoff value was 0.56 (relative reactivity), which resulted in a diagnostic sensitivity and specificity of 100% and 99.7%, respectively. A cutoff value of 0.78 resulted in a test sensitivity of 98.4% and a test specificity of 100%. Overall, our results demonstrate that the glyco-iELISA is highly accurate for diagnosis of porcine brucellosis, improving the diagnostic performance of current serological tests. The recombinant glycoprotein OAg-AcrA can be produced in large homogeneous batches in a standardized way, making it an ideal candidate for further validation as a universal antigen for diagnosis of \u201csmooth\u201d brucellosis in animals and humans.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00151-16","type":"journal-article","created":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T23:34:08Z","timestamp":1458171248000},"page":"1448-1455","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["A Bacterial Glycoengineered Antigen for Improved Serodiagnosis of Porcine Brucellosis"],"prefix":"10.1128","volume":"54","author":[{"given":"Mar\u00eda E.","family":"Cortina","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas Dr. Rodolfo A. Ugalde, Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECH), Universidad Nacional de San Mart\u00edn, CONICET, San Mart\u00edn, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo E.","family":"Balzano","sequence":"additional","affiliation":[{"name":"Laboratorio de Referencia de la OIE para Brucelosis, Direcci\u00f3n General de Laboratorio y Control T\u00e9cnico (DiLab), Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA), Mart\u00ednez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego A.","family":"Rey Serantes","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas Dr. Rodolfo A. Ugalde, Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECH), Universidad Nacional de San Mart\u00edn, CONICET, San Mart\u00edn, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana J.","family":"Caillava","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas Dr. Rodolfo A. Ugalde, Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECH), Universidad Nacional de San Mart\u00edn, CONICET, San Mart\u00edn, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Elena","sequence":"additional","affiliation":[{"name":"Laboratorio de Referencia de la OIE para Brucelosis, Direcci\u00f3n General de Laboratorio y Control T\u00e9cnico (DiLab), Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA), Mart\u00ednez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Unidade Estrat\u00e9gica de Investiga\u00e7\u00e3o e Servi\u00e7os em Produ\u00e7\u00e3o e Sa\u00fade Anima (UEISPSA), Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria (INIAV), Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Nicola","sequence":"additional","affiliation":[{"name":"Laboratorio de Referencia de la OIE para Brucelosis, Direcci\u00f3n General de Laboratorio y Control T\u00e9cnico (DiLab), Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA), Mart\u00ednez, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan E.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas Dr. Rodolfo A. Ugalde, Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECH), Universidad Nacional de San Mart\u00edn, CONICET, San Mart\u00edn, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego J.","family":"Comerci","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas Dr. Rodolfo A. Ugalde, Instituto Tecnol\u00f3gico de Chascom\u00fas (IIB-INTECH), Universidad Nacional de San Mart\u00edn, CONICET, San Mart\u00edn, Buenos Aires, Argentina"},{"name":"Comisi\u00f3n Nacional de Energ\u00eda At\u00f3mica, Grupo Pecuario, Centro At\u00f3mico Ezeiza, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s E.","family":"Ciocchini","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas Dr. Rodolfo A. 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