{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T04:00:27Z","timestamp":1785038427512,"version":"3.55.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with\n            <jats:italic>Entamoeba histolytica<\/jats:italic>\n            . Fecal testing for\n            <jats:italic>E. histolytica<\/jats:italic>\n            parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection of\n            <jats:italic>E. histolytica<\/jats:italic>\n            DNA in blood, urine, and saliva samples from amebic liver abscess as well as amebic colitis patients in Bangladesh. A total of 98 amebic liver abscess and 28 amebic colitis patients and 43 control subjects were examined. The real-time PCR assay detected\n            <jats:italic>E. histolytica<\/jats:italic>\n            DNA in 49%, 77%, and 69% of blood, urine, and saliva specimens from the amebic liver abscess patients. For amebic colitis the sensitivity of the real-time PCR assay for detection of\n            <jats:italic>E. histolytica<\/jats:italic>\n            DNA in blood, urine, and saliva was 36%, 61%, and 64%, respectively. All blood, urine, and saliva samples from control subjects were negative by the real-time PCR assay for\n            <jats:italic>E. histolytica<\/jats:italic>\n            DNA. When the real-time PCR assay results of the urine and saliva specimens were taken together (positive either in urine or saliva), the real-time PCR assay was 97% and 89% sensitive for detection of\n            <jats:italic>E. histolytica<\/jats:italic>\n            DNA in liver abscess and intestinal infection, respectively. We conclude that the detection of\n            <jats:italic>E. histolytica<\/jats:italic>\n            DNA in saliva and urine could be used as a diagnostic tool for amebic liver abscess.\n          <\/jats:p>","DOI":"10.1128\/jcm.00152-10","type":"journal-article","created":{"date-parts":[[2010,6,10]],"date-time":"2010-06-10T01:53:55Z","timestamp":1276134835000},"page":"2798-2801","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Diagnosis of Amebic Liver Abscess and Amebic Colitis by Detection of\n            <i>Entamoeba histolytica<\/i>\n            DNA in Blood, Urine, and Saliva by a Real-Time PCR Assay"],"prefix":"10.1128","volume":"48","author":[{"given":"Rashidul","family":"Haque","sequence":"first","affiliation":[{"name":"International Centre For Diarrhoeal Disease Research, Bangladesh, Dhaka-1000, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamun","family":"Kabir","sequence":"additional","affiliation":[{"name":"International Centre For Diarrhoeal Disease Research, Bangladesh, Dhaka-1000, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zannatun","family":"Noor","sequence":"additional","affiliation":[{"name":"International Centre For Diarrhoeal Disease Research, Bangladesh, Dhaka-1000, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M. Mazidur","family":"Rahman","sequence":"additional","affiliation":[{"name":"International Centre For Diarrhoeal Disease Research, Bangladesh, Dhaka-1000, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh","family":"Mondal","sequence":"additional","affiliation":[{"name":"International Centre For Diarrhoeal Disease Research, Bangladesh, Dhaka-1000, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal","family":"Alam","sequence":"additional","affiliation":[{"name":"Rajshahi Medical College, Rajshahi, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Intekhab","family":"Rahman","sequence":"additional","affiliation":[{"name":"Rajshahi Medical College, Rajshahi, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullh","family":"Al Mahmood","sequence":"additional","affiliation":[{"name":"Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nooruddin","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"William A.","family":"Petri","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease and International Health, University of Virginia Health System, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.6.2344-2347.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4413-4417.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.5.1108-1111.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.1117-1121.1998"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.1031"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.449-452.1998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022710"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3235-3239.2000"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.10.2558-2561.1995"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.713"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1972.21.157"},{"key":"e_1_3_2_13_2","first-page":"1215","year":"2005","unstructured":"Kasper, D. 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