{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:54:36Z","timestamp":1791298476724,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82172311"],"award-info":[{"award-number":["82172311"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and technology innovation action plan of Shanghai","award":["21DZ2200800"],"award-info":[{"award-number":["21DZ2200800"]}]},{"name":"CHINET","award":["2020QD049"],"award-info":[{"award-number":["2020QD049"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2024,7,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The increasing use of ceftazidime-avibactam has led to the emergence of a wide range of ceftazidime-avibactam-resistant\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      variants. Particularly, the conventional carbapenemase phenotypic assay exhibited a high false-negative rate for KPC-2 variants. In this study, three colloidal gold immunoassays, including the Gold Mountainriver CGI test, Dynamiker CGI test and NG-Test CARBA5, and GeneXpert Carba-R, were used to detect the presence of KPC-2 carbapenemase and its various variants in 42\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      strains. These strains covered\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      (13\/42) and 16 other\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      variants including\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-12<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-23<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-25<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-33<\/jats:sub>\n                      (6\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-35<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-44<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-71<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-76<\/jats:sub>\n                      (8\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-78<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-79<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-100<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-127<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-128<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-144<\/jats:sub>\n                      (1\/42),\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-157<\/jats:sub>\n                      (2\/42), and\n                      <jats:italic>bla<\/jats:italic>\n                      <jats:sub>KPC-180<\/jats:sub>\n                      (1\/42). For KPC-2 strains, all four assays showed 100% negative percentage agreement (NPA) and 100% positive percentage agreement (PPA) with sequencing results. For all 16 KPC-2 variants, GeneXpert Carba-R showed 100% NPA and 100% PPA, and the three colloidal gold immunoassays showed 100% NPA, while the PPAs of the Gold Mountainriver CGI test, Dynamiker CGI test, and NG-Test CARBA5 were 87.5%, 87.5%, and 68.8%, respectively. We also found a correlation between the mutation site in the amino acid of the variants and false-negative results by colloidal gold immunoassays. In conclusion, the GeneXpert Carba-R has been proven to be a reliable method in detecting KPC-2 and its variants, and the colloidal gold immunoassay tests offer a practical and cost-effective approach for their detection. For the sample with a negative result by a colloidal gold immunoassay test but not matching the drug-resistant phenotype, it is recommended to retest using another type of kit or the GeneXpert Carba-R assay, which can significantly improve the accuracy of detection.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.00154-24","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T09:04:35Z","timestamp":1716973475000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of three colloidal gold immunoassays and GeneXpert Carba-R for the detection of\n                    <i>Klebsiella pneumoniae bla<\/i>\n                    <sub>KPC-2<\/sub>\n                    variants"],"prefix":"10.1128","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7363-7286","authenticated-orcid":true,"given":"Jinghao","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China"},{"name":"Department of Laboratory Medicine, Huadong Hospital, Fudan University, Shanghai, China"},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, China"},{"name":"Joint Laboratory of Hospital &amp; 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