{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T21:06:29Z","timestamp":1785877589051,"version":"3.56.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Babesiosis in a sable antelope (\n            <jats:italic>Hippotragus niger<\/jats:italic>\n            Harris, 1838) was first reported in 1930; the parasite was named\n            <jats:italic>Babesia irvinesmithi<\/jats:italic>\n            . Recently, specimens from an adult sable that presented with a sudden onset of disease and that subsequently died during immobilization were submitted for molecular characterization. Microscopic examination of thin blood smears revealed the presence of small piroplasms. DNA was extracted from blood samples; the V4 variable region of the 18S rRNA gene was amplified and analyzed using the reverse line blot (RLB) assay. Amplicons did not hybridize with any of the\n            <jats:italic>Babesia<\/jats:italic>\n            or\n            <jats:italic>Theileria<\/jats:italic>\n            species-specific probes present on the blot and hybridized only with a\n            <jats:italic>Babesia<\/jats:italic>\n            or\n            <jats:italic>Theileria<\/jats:italic>\n            genus-specific probe, suggesting the presence of a novel species. The full-length 18S rRNA gene sequence was obtained and aligned with published sequences of related genera, and phylogenetic trees were constructed. Sequence similarity analyses indicated that a\n            <jats:italic>Babesia<\/jats:italic>\n            species, designated\n            <jats:italic>Babesia<\/jats:italic>\n            sp. (sable), was present. The sequence showed its highest similarity to\n            <jats:italic>B. orientalis<\/jats:italic>\n            and to an unnamed\n            <jats:italic>Babesia<\/jats:italic>\n            species previously detected in bovine samples. The latter was later established to be\n            <jats:italic>Babesia occultans<\/jats:italic>\n            . A\n            <jats:italic>Babesia<\/jats:italic>\n            sp. (sable)-specific RLB oligonucleotide probe was designed and used to screen 200 South African sable samples, but so far, no other sample has been found to be positive for the presence of\n            <jats:italic>Babesia<\/jats:italic>\n            sp. (sable) DNA. In summary, we identified a novel piroplasm parasite from a sable antelope that died from an unknown illness. While the parasite was observed in blood smears, there is no direct evidence that it was the cause of death.\n          <\/jats:p>","DOI":"10.1128\/jcm.00167-08","type":"journal-article","created":{"date-parts":[[2008,5,29]],"date-time":"2008-05-29T00:53:49Z","timestamp":1212022429000},"page":"2247-2251","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":112,"title":["Identification of a Novel\n            <i>Babesia<\/i>\n            sp. from a Sable Antelope (\n            <i>Hippotragus niger<\/i>\n            Harris, 1838)"],"prefix":"10.1128","volume":"46","author":[{"given":"Marinda C.","family":"Oosthuizen","sequence":"first","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich","family":"Zweygarth","sequence":"additional","affiliation":[{"name":"Agricultural Research Council-Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola E.","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milana","family":"Troskie","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banie L.","family":"Penzhorn","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"The phylogeny of Babesia Theileria and related parasites and the development of species-specific probes for diagnostic purposes. 1994"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1373.076"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00179-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4172-4177.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.24.4992"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2028.1965.tb00745.x"},{"key":"e_1_3_2_8_2","unstructured":"Appendices I II and III. 2007"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_3_2_10_2","first-page":"215","volume":"48","year":"1981","unstructured":"Gray, J. 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