{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:46:35Z","timestamp":1791218795008,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Classically,\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            infection is associated with high levels of specific IgM antibody and a rise in specific IgG levels 1 to 3 weeks later. Atypical IgG seroconversion, without IgM detection or with transient IgM levels, has been described during serologic follow-up of seronegative pregnant women and raises difficulties in interpreting the results. To evaluate the frequency and the characteristics of these atypical cases of seroconversion, an investigation was conducted within the French National Reference Center for Toxoplasmosis, from which 26 cases collected from 12 laboratories belonging to the network were identified. The aim of this work was to retrospectively analyze the results of serologic testing, the treatments administered, and the results of prenatal and postnatal follow-up for these women. In each case, IgG antibodies were detected using both screening and confirmatory tests. IgM antibodies were not detected in 15 cases, and the levels were equivocal or low-positive in 11 cases. The IgG avidity results were low in 16 cases and high in one case. Most of the pregnant women (22\/26) were treated with spiramycin from the time that IgG antibodies appeared until delivery. Amniotic fluid was analyzed for\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            DNA by PCR in 11\/26 cases, and the results were negative in all cases. Congenital toxoplasmosis was ruled out in 12\/26 newborns. There was no abnormality observed at birth for 10 newborns and no information available for 4 newborns. In conclusion, when the interpretation of serological results is so difficult, it seems cautious to initiate treatment by spiramycin and to follow the pregnant women and their newborns.\n          <\/jats:p>","DOI":"10.1128\/jcm.00169-13","type":"journal-article","created":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T04:14:37Z","timestamp":1366863277000},"page":"2103-2111","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Toxoplasma Seroconversion with Negative or Transient Immunoglobulin M in Pregnant Women: Myth or Reality? A French Multicenter Retrospective Study"],"prefix":"10.1128","volume":"51","author":[{"given":"H.","family":"Fricker-Hidalgo","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universit\u00e9 Joseph Fourier, Grenoble 1 et Centre Hospitalier Universitaire A. Michallon, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Cimon","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Institut de Biologie en Sant\u00e9, Centre Hospitalier Universitaire, Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Chemla","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, H\u00f4pital Maison Blanche, Centre Hospitalier Universitaire, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. L.","family":"Darde","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universit\u00e9 de Limoges et Centre Hospitalier Universitaire Dupuytren, Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Delhaes","sequence":"additional","affiliation":[{"name":"Service de Parasitologie et Mycologie, H\u00f4pital A. Calmette, Centre Hospitalier Universitaire, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"L'Ollivier","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, H\u00f4pital de la Timone, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Godineau","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier de Saint-Denis, Saint-Denis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Houze","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, H\u00f4pital Claude Bernard Bichat, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Paris","sequence":"additional","affiliation":[{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Laboratoire de Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Quinio","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier R\u00e9gional Universitaire, Brest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Robert-Gangneux","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Villard","sequence":"additional","affiliation":[{"name":"Institut de Parasitologie et de Pathologie Tropicale de Strasbourg, Universit\u00e9 de Strasbourg, H\u00f4pitaux Universitaires de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Villena","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, H\u00f4pital Maison Blanche, Centre Hospitalier Universitaire, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Candolfi","sequence":"additional","affiliation":[{"name":"Institut de Parasitologie et de Pathologie Tropicale de Strasbourg, Universit\u00e9 de Strasbourg, H\u00f4pitaux Universitaires de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Pelloux","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universit\u00e9 Joseph Fourier, Grenoble 1 et Centre Hospitalier Universitaire A. Michallon, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.09.005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05013-11"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.10.018"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762009000200029"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2010.06.002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3182343096"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.02.004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","unstructured":"VillenaI\nAncelleT\nDelmasC\nGarciaP\nBrezinAP\nThulliezP\nWallonM\nKingL\nGouletV. 2010. Congenital toxoplasmosis in France in 2007: first results from a national surveillance system. Euro Surveill. 15:pii=19600. http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId=19600.","DOI":"10.2807\/ese.15.25.19600-en"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-011-0069-x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/09273948.2010.605198"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2012.693197"},{"key":"e_1_3_2_13_2","first-page":"43","article-title":"S\u00e9rodiagnostic de la toxoplamose en 2010: conduite \u00e0 tenir et interpr\u00e9tation en fonction des profils s\u00e9rologiques obtenus par les m\u00e9thodes de d\u00e9pistage","volume":"298","author":"Villard O","year":"2011","unstructured":"VillardO Jung-EtienneJ CimonB FranckJ Fricker-HidalgoH GodineauN HouzeS ParisL PellouxH VillenaI CandolfiE, le r\u00e9seau du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose. 2011. S\u00e9rodiagnostic de la toxoplamose en 2010: conduite \u00e0 tenir et interpr\u00e9tation en fonction des profils s\u00e9rologiques obtenus par les m\u00e9thodes de d\u00e9pistage. Feuill. Biol. 298:43\u201349.","journal-title":"Feuill. Biol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00025-08"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00253-09"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01444.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(98)00077-7"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.006668-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.12.010"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01076-08"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01905.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.04.001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.07.013"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-006-0204-1"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/590149"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05303-11"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00182-08"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00128-09"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2187-2187.1997"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3423-3424.1999"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.11.6.562-568.1980"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.3.579-583.1996"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762009000200038"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01164-09"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03101.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00011289"},{"key":"e_1_3_2_37_2","first-page":"143","article-title":"How to resolve the difficulties encountered in the serodiagnosis of toxoplasmosis in pregnant women?","volume":"17","author":"Cimon B","year":"2002","unstructured":"CimonB PennP BrunS ChabasseD. 2002. How to resolve the difficulties encountered in the serodiagnosis of toxoplasmosis in pregnant women? Immunol. Biol. Spec. 17:143\u2013147. (In French.)","journal-title":"Immunol. Biol. Spec"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1086\/595793"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.03.012"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00169-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00169-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:35:46Z","timestamp":1646480146000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00169-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7]]},"references-count":38,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1128\/JCM.00169-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00169-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7]]},"assertion":[{"value":"2013-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}