{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T06:45:37Z","timestamp":1777963537938,"version":"3.51.4"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            High-risk human papillomavirus (HPV) DNA\/RNA testing provides higher sensitivity but lower specificity than cytology for the identification of high-grade cervical intraepithelial neoplasia (CIN). Several new HPV tests are now available for this purpose, and a direct comparison of their properties is needed. Seven tests were evaluated with samples in liquid PreservCyt transport medium from 1,099 women referred for colposcopy: the Hybrid Capture 2 (Qiagen), Cobas (Roche), PreTect HPV-Proofer (NorChip), Aptima HPV (Gen-Probe), and Abbott RealTi\n            <jats:italic>m<\/jats:italic>\n            e assays, the BD HPV test, and CINtec p16\n            <jats:sup>INK4a<\/jats:sup>\n            cytology (mtm laboratories) immunocytochemistry. Sensitivity, specificity, and positive predictive value (PPV) were based on the worst histology found on either the biopsy or the treatment specimen after central review. Three hundred fifty-nine women (32.7%) had CIN grade 2+ (CIN2+), with 224 (20.4%) having CIN3+. For detection of CIN2+, Hybrid Capture 2 had 96.3% sensitivity, 19.5% specificity, and 37.4% PPV. Cobas had 95.2% sensitivity, 24.0% specificity, and 37.6% PPV. The BD HPV test had 95.0% sensitivity, 24.2% specificity, and 37.8% PPV. Abbott RealTi\n            <jats:italic>m<\/jats:italic>\n            e had 93.3% sensitivity, 27.3% specificity, and 38.2% PPV. Aptima had 95.3% sensitivity, 28.8% specificity, and 39.3% PPV. PreTect HPV-Proofer had 74.1% sensitivity, 70.8% specificity, and 55.4% PPV. CINtec p16\n            <jats:sup>INK4a<\/jats:sup>\n            cytology had 85.7% sensitivity, 54.7% specificity, and 49.1% PPV. Cytology of a specimen taken at colposcopy (mild dyskaryosis or worse) had 88.9% sensitivity, 58.1% specificity, and 50.7% PPV. Our study confirms that, in a referral setting, HPV testing by a number of different tests provides high sensitivity for high-grade disease. Further work is needed to confirm these findings in a routine screening setting.\n          <\/jats:p>","DOI":"10.1128\/jcm.00181-12","type":"journal-article","created":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T01:00:51Z","timestamp":1331773251000},"page":"1867-1873","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":145,"title":["Comparison of Seven Tests for High-Grade Cervical Intraepithelial Neoplasia in Women with Abnormal Smears: the Predictors 2 Study"],"prefix":"10.1128","volume":"50","author":[{"given":"Anne","family":"Szarewski","sequence":"first","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Mesher","sequence":"additional","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Cadman","sequence":"additional","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet","family":"Austin","sequence":"additional","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lesley","family":"Ashdown-Barr","sequence":"additional","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Ho","sequence":"additional","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Terry","sequence":"additional","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart","family":"Liddle","sequence":"additional","affiliation":[{"name":"The Doctors Laboratory, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Cellular Pathology, Royal Free Hampstead NHS Trust, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Stoler","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Cytopathology Department, St. Mary's Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corrina","family":"Wright","sequence":"additional","affiliation":[{"name":"Cytopathology Department, St. Mary's Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Bergeron","sequence":"additional","affiliation":[{"name":"Laboratoire Pasteur-Cerba, Cergy Pontoise, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. P","family":"Soutter","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, Hammersmith and Queen Charlotte's &amp; Chelsea Hospitals, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deirdre","family":"Lyons","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, St. Mary's Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack","family":"Cuzick","sequence":"additional","affiliation":[{"name":"Centre for Cancer Prevention, Wolfson Institute of Preventive Medicine, Queen Mary, University of London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22757"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24774"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61450-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602541"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02311-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00422-11"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21801"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21955"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14955-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP3CD9YKYFJDQL"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-199903000-00005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.a1754"},{"key":"e_1_3_2_14_2","volume-title":"International Papillomavirus Conference","author":"Joo S","year":"2010","unstructured":"JooS SalvaN NaterT DockterJ. 2010. 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