{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T16:00:59Z","timestamp":1782316859877,"version":"3.54.5"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Nucleic acid amplification testing (NAAT) has become the preferred method to detect\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            , but no commercial tests are cleared by the U.S. Food and Drug Administration for use with rectal swab samples. This study evaluated the performance of strand displacement amplification (SDA) and transcription-mediated amplification (TMA) to detect\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            and to determine if TMA could also detect\n            <jats:named-content content-type=\"genus-species\">Mycoplasma genitalium<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Trichomonas vaginalis<\/jats:named-content>\n            in men and women reporting a history of receptive anal intercourse. Discordant results between the NAATs were reevaluated using the Aptima CT or Aptima GC assay, each of which targets primers other than those targeted by the Aptima Combo 2 (AC2) assay, as the confirmatory test. Of 497 evaluable participants, 41 (8.2%) were positive for\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            , 21 (4.2%) were positive for\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            , 26 (5.2%) were positive for\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            , and 47 (9.5%) were positive for\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            . The sensitivity and specificity of the\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            test were 100% and 99.8% for AC2 and 56.1% and 100% for SDA, respectively. The sensitivity and specificity of the\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            test were 100% and 100% for AC2 and 76.2% and 100% for SDA, respectively, while culture was only 23.8% sensitive. Of the 114 participants who had a positive result for any of the four infectious agents, 16 were positive for two pathogens and 3 were positive for three pathogens. These data suggest that rectal infection is common and that the AC2 is superior to SDA for the detection of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            from rectal swab samples.\n          <\/jats:p>","DOI":"10.1128\/jcm.00185-12","type":"journal-article","created":{"date-parts":[[2012,4,5]],"date-time":"2012-04-05T04:28:21Z","timestamp":1333600101000},"page":"2005-2008","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Use of Nucleic Acid Amplification Testing for Diagnosis of Anorectal Sexually Transmitted Infections"],"prefix":"10.1128","volume":"50","author":[{"given":"Lisa A.","family":"Cosentino","sequence":"first","affiliation":[{"name":"Magee-Womens Research Institute, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"},{"name":"Microbicide Trials Network, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Campbell","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abi","family":"Jett","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Macio","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"},{"name":"Microbicide Trials Network, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Zamborsky","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross D.","family":"Cranston","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Hillier","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"},{"name":"Microbicide Trials Network, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"},{"name":"Departments of Obstetrics, Gynecology and Reproductive Sciences and Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.031443"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02398-09"},{"key":"e_1_3_2_4_2","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2010","volume":"59","author":"Centers for Disease Control and Prevention","year":"2010","unstructured":"Centers for Disease Control and Prevention. 2010. Sexually transmitted diseases treatment guidelines, 2010. MMWR Morb. Mortal. Wkly. Rep. 59:1\u2013109.","journal-title":"MMWR Morb. Mortal. Wkly. 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