{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:54:18Z","timestamp":1791251658884,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sixty-two clinical isolates of\n            <jats:italic>Enterobacter aerogenes<\/jats:italic>\n            resistant to expanded-spectrum cephalosporins were collected between July 2003 and May 2005. Among these isolates, 23 (37.1%) were imipenem (IPM) susceptible, and 39 (62.9%) were IPM insusceptible, of which 89.7% (35\/39) were resistant and 10.3% (4\/39) were intermediate. Isolate genotypes were compared by pulsed-field gel electrophoresis. Of 62 isolates, 48 belonged to epidemic pulsotype A (77.4%). This pulsotype included 37.5% and 58.4% of \u03b2-lactam phenotypes b and a, respectively. Nine isolates (14.5%) belonged to pulsotype E, which included 22.3% and 77.7% of phenotypes b and a, respectively. The \u03b2-lactamases with pIs of 5.4, 6.5, 8.2, and 8.2 corresponded to extended-spectrum \u03b2-lactamases (ESBLs) TEM-20, TEM-24, SHV-5, and SHV-12, respectively. Of 39 IPM-insusceptible\n            <jats:italic>E. aerogenes<\/jats:italic>\n            isolates, 26 (66.6%) were determined to be metallo-\u03b2-lactamase producers, by using a phenotypic method. Of these isolates, 24 harbored a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-1<\/jats:sub>\n            gene encoding a protein with a pI of &gt;9.5, and two carried the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VIM-2<\/jats:sub>\n            gene encoding a protein with a pI of 5.3, corresponding to \u03b2-lactamases IMP-1 and VIM-2, respectively. The remaining 13 (33.4%) isolates were negative for the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMP-1<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>VIM-2<\/jats:sub>\n            genes but showed an alteration of their outer membrane proteins (OMPs). Ten of these isolates produced the two possible OMPs (32 and 42 kDa), with IPM MICs between 8 and 32 \u03bcg\/ml, and three others produced only a 32-kDa OMP with IPM MICs &gt;32 \u03bcg\/ml. This work demonstrates that, in addition to resistance to expanded-spectrum cephalosporins, IPM resistance can occur in ESBL-producing\n            <jats:italic>E. aerogenes<\/jats:italic>\n            isolates by carbapenemase production or by the loss of porin in the outer membrane.\n          <\/jats:p>","DOI":"10.1128\/jcm.00197-07","type":"journal-article","created":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T02:04:48Z","timestamp":1201745088000},"page":"1037-1044","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Successive Emergence of Extended-Spectrum \u03b2-Lactamase-Producing and Carbapenemase-Producing\n            <i>Enterobacter aerogenes<\/i>\n            Isolates in a University Hospital"],"prefix":"10.1128","volume":"46","author":[{"given":"M.","family":"Biendo","sequence":"first","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Canarelli","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"},{"name":"Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 80036 Amiens, Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Hamdad","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Adjide","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Laurans","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"},{"name":"Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 80036 Amiens, Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Eb","sequence":"additional","affiliation":[{"name":"Service de Bacte\u0301riologie et d'Hygie\u0300ne CHU NORD, Place Victor Pauchet, 80054 Amiens, Cedex 1 France"},{"name":"Laboratoire de Bacte\u0301riologie, Faculte\u0301 de Me\u0301decine, 80036 Amiens, Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.3.852-860.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.969"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.9.2163-2169.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.7.2170-2175.1999"},{"key":"e_1_3_2_6_2","first-page":"112","year":"2006","unstructured":"Biendo, M., G. 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