{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:01:14Z","timestamp":1791295274019,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The GenoType MTBDR\n                    <jats:italic>sl<\/jats:italic>\n                    test and DNA sequencing were used to rapidly detect second-line drug- and ethambutol (EMB)-resistant\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    . The ability of these two assays to detect the presence of mutations associated with resistance to fluoroquinolones (FLQ), aminoglycosides\/cyclic peptide (AG\/CP), and EMB in the\n                    <jats:italic>gyrA<\/jats:italic>\n                    ,\n                    <jats:italic>rrs<\/jats:italic>\n                    , and\n                    <jats:italic>embB<\/jats:italic>\n                    genes (for the GenoType MTBDR\n                    <jats:italic>sl<\/jats:italic>\n                    test) and\n                    <jats:italic>gyrA<\/jats:italic>\n                    ,\n                    <jats:italic>gyrB<\/jats:italic>\n                    ,\n                    <jats:italic>rrs<\/jats:italic>\n                    ,\n                    <jats:italic>eis<\/jats:italic>\n                    ,\n                    <jats:italic>embC<\/jats:italic>\n                    ,\n                    <jats:italic>embA<\/jats:italic>\n                    ,\n                    <jats:italic>embB<\/jats:italic>\n                    , and\n                    <jats:italic>embR<\/jats:italic>\n                    genes (for DNA sequencing) was compared to that of conventional agar proportion drug susceptibility testing (DST). We evaluated 234 multidrug-resistant (MDR)\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    isolates. The two molecular methods had high levels of specificity (95.8 to 100%). The sensitivities for FLQ resistance detection for both methods were 85.1%. For AG (kanamycin [KM] and amikacin [AM]) and CP (capreomycin CAP]), the sensitivities of resistance detection using the GenoType MTBDR\n                    <jats:italic>sl<\/jats:italic>\n                    test were 43.2%, 84.2%, and 71.4%, respectively, while with the inclusion of an extra gene,\n                    <jats:italic>eis<\/jats:italic>\n                    , in sequencing, the sensitivity reached 70.3% for detection of KM resistance. The sensitivities of EMB resistance detection were 56.2% and 90.7% with the GenoType MTBDR\n                    <jats:italic>sl<\/jats:italic>\n                    test and sequencing, respectively. We found that the GenoType MTBDR\n                    <jats:italic>sl<\/jats:italic>\n                    test can rapidly detect resistance to FLQ, CAP, and AM. The accuracy of the GenoType MTBDR\n                    <jats:italic>sl<\/jats:italic>\n                    test for the detection of FLQ and AM resistance was comparable to that of conventional DST; however, the test was less accurate for the detection of KM and EMB resistance and demonstrated a poor predictive value for CAP resistance. We recommend including new alleles consisting of the\n                    <jats:italic>eis<\/jats:italic>\n                    promoter and\n                    <jats:italic>embB<\/jats:italic>\n                    genes in molecular analysis. However, conventional DST is necessary to rule out false-negative results from molecular assays.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00197-11","type":"journal-article","created":{"date-parts":[[2011,5,11]],"date-time":"2011-05-11T20:16:19Z","timestamp":1305144979000},"page":"2502-2508","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Performance Assessment of the GenoType MTBDR\n                    <i>sl<\/i>\n                    Test and DNA Sequencing for Detection of Second-Line and Ethambutol Drug Resistance among Patients Infected with Multidrug-Resistant Mycobacterium tuberculosis"],"prefix":"10.1128","volume":"49","author":[{"given":"Wei-Lun","family":"Huang","sequence":"first","affiliation":[{"name":"Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Lin","family":"Chi","sequence":"additional","affiliation":[{"name":"Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Hua","family":"Wu","sequence":"additional","affiliation":[{"name":"Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruwen","family":"Jou","sequence":"additional","affiliation":[{"name":"Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2006.05.004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1295"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2270"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10517-008-0034-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01947-09"},{"key":"e_1_3_2_7_2","unstructured":"Centers for Disease Control Department of Health Executive Yuan. 2009. 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