{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T14:29:43Z","timestamp":1780410583551,"version":"3.54.1"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An external quality assessment (EQA) panel consisting of a total of 48 samples in bronchoalveolar lavage (BAL) fluid or transport medium was prepared in collaboration with Quality Control for Molecular Diagnostics (QCMD) (\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.qcmd.org\">www.qcmd.org<\/jats:ext-link>\n            ). The panel was used to assess the proficiency of the three laboratories that would be responsible for examining the 6,000 samples to be collected in the GRACE Network of Excellence (\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.grace-lrti.org\">www.grace-lrti.org<\/jats:ext-link>\n            ). The main objective was to decide on the best-performing testing approach for the detection of influenza viruses A and B, parainfluenza virus types 1 to 3, respiratory syncytial virus (RSV), human metapneumovirus, coronavirus, rhinovirus, adenovirus,\n            <jats:named-content content-type=\"genus-species\">Chlamydophila pneumoniae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Mycoplasma pneumoniae<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            by nucleic acid amplification techniques (NAATs). Two approaches were chosen: (i) laboratories testing samples using their in-house procedures for extraction and amplification and (ii) laboratories using their in-house amplification procedures on centrally extracted samples. Furthermore, three commercially available multiplex NAAT tests\u2014the ResPlex (Qiagen GmbH, Hilden, Germany), RespiFinder plus (PathoFinder, Maastricht, The Netherlands), and RespiFinder Smart 21 (PathoFinder) tests\u2014were evaluated by examination of the same EQA panel by the manufacturer. No large differences among the 3 laboratories were noticed when the performances of the assays developed in-house in combination with the in-house extraction procedures were compared. Also, the extraction procedure (central versus local) had little effect on performance. However, large differences in amplification efficacy were found between the commercially available tests; acceptable results were obtained by using the PathoFinder assays.\n          <\/jats:p>","DOI":"10.1128\/jcm.00200-11","type":"journal-article","created":{"date-parts":[[2011,12,15]],"date-time":"2011-12-15T04:57:46Z","timestamp":1323925066000},"page":"977-987","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Performance of Different Mono- and Multiplex Nucleic Acid Amplification Tests on a Multipathogen External Quality Assessment Panel"],"prefix":"10.1128","volume":"50","author":[{"given":"K.","family":"Loens","sequence":"first","affiliation":[{"name":"Department of Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"van Loon","sequence":"additional","affiliation":[{"name":"Department of Virology, Medical Microbiology, University Medical Center, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Coenjaerts","sequence":"additional","affiliation":[{"name":"Department of Virology, Medical Microbiology, University Medical Center, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"van Aarle","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Goossens","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Wallace","sequence":"additional","affiliation":[{"name":"Quality Control for Molecular Diagnostics, Glasgow, Scotland, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. J. C.","family":"Claas","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ieven","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2010.09.022"},{"key":"e_1_3_2_3_2","volume-title":"QCMD report.","author":"De Mendonca R","year":"2007","unstructured":"De MendoncaR MacKayWG. 2007. QCMD 2007 Legionella pneumophila (LPDNA07) proficiency programme. QCMD report. QCMD, Glasgow, Scotland."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03529.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21771"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/511076"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.02.010"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00894-06"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0975-2"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.05.006"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00037-07"},{"key":"e_1_3_2_12_2","unstructured":"MeijerA\nNiestersH\nMacKayWG. 2007. QCMD 2007 influenzavirus A and B (INFA07) proficiency programme. QCMD Glasgow Scotland."},{"key":"e_1_3_2_13_2","unstructured":"NiestersH\nMacKayWG\nvan LoonAM. 2007. QCMD 2007 influenza virus haemagglutinin typing INFHT07 proficiency programme. QCMD Glasgow Scotland."},{"key":"e_1_3_2_14_2","volume-title":"QCMD report.","author":"Niesters H","year":"2007","unstructured":"NiestersH van LoonAM MacKayWG. 2007. QCMD 2007 metapneumovirus and respiratory syncytial virus (MPVRSV07) proficiency programme. QCMD report. QCMD, Glasgow, Scotland."},{"key":"e_1_3_2_15_2","volume-title":"QCMD 2007 adenovirus (ADVDNA07) proficiency programme.","author":"Pandit A","year":"2007","unstructured":"PanditA ClaasEC. 2007. QCMD 2007 adenovirus (ADVDNA07) proficiency programme. QCMD, Glasgow, Scotland."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.08.021"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1564-1569.2004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/431588"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(03)00131-3"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00097-10"},{"key":"e_1_3_2_21_2","unstructured":"van LoonAM\nMacKayWG. 2006. QCMD 2006 influenza virus A and B (INFRNA06) proficiency programme. QCMD Glasgow Scotland."},{"key":"e_1_3_2_22_2","unstructured":"van LoonAM\nNiestersH\nMacKayWG. 2007. QCMD 2007 rhinovirus and coronavirus (RVCVRNA07) proficiency programme. QCMD Glasgow Scotland."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.3.1483-1486.2004"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00200-11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00200-11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:22:25Z","timestamp":1646479345000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00200-11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1128\/JCM.00200-11"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00200-11","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]},"assertion":[{"value":"2011-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-10-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}