{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:30:44Z","timestamp":1791167444558,"version":"4.1.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The MTBDRsl assay (Hain Lifescience GmbH, Germany) is a new line probe assay for the detection of extensively drug-resistant tuberculosis (XDR TB). The test simultaneously detects resistance to ethambutol, aminoglycosides\/cyclic peptides, and fluoroquinolones through detection of mutations in the relevant genes. The assay format is identical to the MTBDR Hain assay. The assay was evaluated for the detection of second-line-drug resistance in Vietnamese isolates using two sample sets from the microbiology department of Pham Ngoc Thach Hospital, Ho Chi Minh City, Viet Nam, with existing conventional phenotypic drug susceptibility results for second-line drugs: 41 consecutive fluoroquinolone-resistant isolates and 21 consecutive multidrug-resistant but fluoroquinolone-sensitive isolates. The sensitivity for detection of fluoroquinolone resistance was 75.6% (31\/41) (95% confidence interval [95% CI], 59.7% to 87.6%), and for kanamycin resistance, the sensitivity was 100% (5\/5) (95% CI, 47.8% to 100%). The sensitivity of the test for detection of ethambutol resistance was low, consistent with previous reports, at 64.2% (34\/53) (95% CI, 49.8% to 76.9%). The specificity of the test was 100% for all three drugs. These data suggest that the MTBDRsl assay is a rapid, specific test for the detection of XDR TB but should not be used exclusively to \u201crule out\u201d second-line-drug resistance. Further operational evaluation is required and should be integrated with evaluations of the MTBDR test.<\/jats:p>","DOI":"10.1128\/jcm.00201-10","type":"journal-article","created":{"date-parts":[[2010,6,24]],"date-time":"2010-06-24T02:40:46Z","timestamp":1277347246000},"page":"2934-2939","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Evaluation of the MTBDRsl Test for Detection of Second-Line-Drug Resistance in\n            <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Vo Sy","family":"Kiet","sequence":"first","affiliation":[{"name":"Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi Ngoc","family":"Lan","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital for Tuberculosis and Lung Diseases, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duong Duy","family":"An","sequence":"additional","affiliation":[{"name":"Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Huy","family":"Dung","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital for Tuberculosis and Lung Diseases, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dai Viet","family":"Hoa","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital for Tuberculosis and Lung Diseases, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen","family":"van Vinh Chau","sequence":"additional","affiliation":[{"name":"Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Tran","family":"Chinh","sequence":"additional","affiliation":[{"name":"Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Farrar","sequence":"additional","affiliation":[{"name":"Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, Old Road, Headington, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxine","family":"Caws","sequence":"additional","affiliation":[{"name":"Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben Ham Tu, District 5, Ho Chi Minh City, Vietnam"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, Old Road, Headington, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.10.2270"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200709-1436OC"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812472106"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01947-09"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00330-06"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69573-1"},{"key":"e_1_3_1_8_2","unstructured":"Genotype MTBDRsl version 1.0 manual. 2009"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00081-09"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00803-09"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/857901"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00036-08"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-5-62"},{"key":"e_1_3_1_14_2","first-page":"520","volume":"47","year":"2009","unstructured":"Sugawara, I., J. Zhang, and C. Li. 2009. Cross-resistance of Mycobacterium tuberculosis isolates among streptomycin, kanamycin and amikacin. Indian J. Exp. Biol. 47 : 520-522.","journal-title":"Indian J. Exp. Biol."},{"key":"e_1_3_1_15_2","first-page":"736","volume":"12","year":"2008","unstructured":"van Doorn, H. R., D. D. An, M. D. de Jong, N. T. Lan, D. V. Hoa, H. T. Quy, N. V. Chau, P. M. Duy, D. Q. Tho, N. T. Chinh, J. J. Farrar, and M. Caws. 2008. Fluoroquinolone resistance detection in Mycobacterium tuberculosis with locked nucleic acid probe real-time PCR. Int. J. Tuber. Lung Dis. 12 : 736-742.","journal-title":"Int. J. Tuber. Lung Dis."},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01476-09"},{"key":"e_1_3_1_17_2","unstructured":"World Health Organization. 2009. Global Tuberculosis Control-epidemiology strategy and financing. WHO report 2009. WHO\/HTM\/TB\/2009.411. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/global_report\/2009\/en\/\n."},{"key":"e_1_3_1_18_2","unstructured":"World Health Organization. 2008. Anti-tuberculosis drug resistance in the world. Fourth Global Report. WHO\/HTM\/TB\/2008.394. The World Health Organization\/International Union Against Tuberculosis and Lung Disease (WHO\/UNION) Global Project on Anti-Tuberculosis Drug Resistance Surveillance 2002-2007. World Health Organization Geneva Switzerland. http:\/\/whqlibdoc.who.int\/hq\/2008\/WHO_HTM_TB_2008.394_eng.pdf\n."},{"key":"e_1_3_1_19_2","unstructured":"World Health Organization. 2008. Molecular line probe assays for rapid screening of patients at risk of multi-drug resistant tuberculosis (MDR TB). Expert Group Report. World Health Organization and the UNICEF\/UNDP\/World Bank\/WHO Special Programme for Research and Training in Tropical Diseases (TDR). World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/features_archive\/expert_group_report_june08.pdf\n."},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907925106"},{"key":"e_1_3_1_21_2","first-page":"1320","volume":"13","year":"2009","unstructured":"Zhang, Y., and W. W. Yew. 2009. Mechanisms of drug resistance in Mycobacterium tuberculosis. Int. J. Tuber. Lung Dis. 13 : 1320-1330.","journal-title":"Int. J. Tuber. 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