{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:24:11Z","timestamp":1789809851970,"version":"4.0.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In French Guiana, leishmaniasis is an essentially cutaneous infection. It constitutes a major public health problem, with a real incidence of 0.2 to 0.3%.\n            <jats:named-content content-type=\"genus-species\">Leishmania guyanensis<\/jats:named-content>\n            is the causal species most frequently encountered in French Guiana. The treatment of leishmaniasis is essentially drug based, but the therapeutic compounds available have major side effects (e.g., liver damage and diabetes) and must be administered parenterally or are costly. The efficacy of some of these agents has declined due to the emergence of resistance in certain strains of\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            . There is currently no vaccine against leishmaniasis, and it is therefore both necessary and urgent to identify new compounds effective against\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            . The search for new drugs requires effective tests for evaluations of the leishmanicidal activity of a particular molecule or extract. Microculture tetrazolium assays (MTAs) are colorimetric tests based on the use of tetrazolium salts. We compared the efficacies of three tetrazolium salts\u20143-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2\n            <jats:italic>H<\/jats:italic>\n            -tetrazolium-5-carboxanilide (XTT), and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2\n            <jats:italic>H<\/jats:italic>\n            -tetrazolium (WST-8)\u2014for quantification of the promastigotes of various species of\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            . We found that the capacity of\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            to metabolize a tetrazolium salt depended on the salt used and the species of\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            . WST-8 was the tetrazolium salt best metabolized by\n            <jats:named-content content-type=\"genus-species\">L. guyanensis<\/jats:named-content>\n            and gave the best sensitivity.\n          <\/jats:p>","DOI":"10.1128\/jcm.00201-14","type":"journal-article","created":{"date-parts":[[2014,4,10]],"date-time":"2014-04-10T03:55:23Z","timestamp":1397102123000},"page":"2131-2138","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Comparison of Tetrazolium Salt Assays for Evaluation of Drug Activity against Leishmania spp"],"prefix":"10.1128","volume":"52","author":[{"given":"Marine","family":"Ginouves","sequence":"first","affiliation":[{"name":"Universit\u00e9 des Antilles et de la Guyane, Laboratoire d'\u00c9pid\u00e9miologie des Parasitoses Tropicales EA 3593, Labex CEBA UFR de M\u00e9decine, Cayenne, French Guiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Carme","sequence":"additional","affiliation":[{"name":"Universit\u00e9 des Antilles et de la Guyane, Laboratoire d'\u00c9pid\u00e9miologie des Parasitoses Tropicales EA 3593, Labex CEBA UFR de M\u00e9decine, Cayenne, French Guiana"},{"name":"Laboratoire Hospitalo-Universitaire de Parasitologie et Mycologie, Centre Hospitalier de Cayenne, Cayenne, French Guiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Couppie","sequence":"additional","affiliation":[{"name":"Universit\u00e9 des Antilles et de la Guyane, Laboratoire d'\u00c9pid\u00e9miologie des Parasitoses Tropicales EA 3593, Labex CEBA UFR de M\u00e9decine, Cayenne, French Guiana"},{"name":"Service de Dermatologie, Centre Hospitalier de Cayenne, Cayenne, French Guiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghislaine","family":"Prevot","sequence":"additional","affiliation":[{"name":"Universit\u00e9 des Antilles et de la Guyane, Laboratoire d'\u00c9pid\u00e9miologie des Parasitoses Tropicales EA 3593, Labex CEBA UFR de M\u00e9decine, Cayenne, French Guiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"WHO Technical Report Series 949","author":"WHO","year":"2010","unstructured":"WHO. 2010. 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