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The<jats:italic>emm1<\/jats:italic>\/ST28 strain is associated with necrotizing fasciitis and streptococcal toxic shock syndrome. Growth-phase regulation is one of the important regulatory mechanisms in GAS, which controls gene expression at restricted phases of growth. CovRS, a two-component regulatory system, is considered the regulator of streptococcal pyrogenic exotoxin B (SpeB) and is thought to be activated in the exponential phase of growth. In the present study, Northern hybridization analysis showed that 52% of the analyzed GAS strains expressed<jats:italic>covR<\/jats:italic>at the exponential phase, but 48% of the strains expressed<jats:italic>covR<\/jats:italic>at the early stationary phase of growth. Strains transcribing<jats:italic>covR<\/jats:italic>at the early stationary phase showed better growth and earlier SpeB expression than the other group of strains. Multilocus sequence typing and pulsed-field gel electrophoresis analysis showed only<jats:italic>emm1<\/jats:italic>\/ST28 strains (which comprise a clonal cluster) were expressing<jats:italic>covR<\/jats:italic>at the early stationary phase of growth, indicating that<jats:italic>emm1<\/jats:italic>\/ST28 strains have special characteristics which may be related to their worldwide distribution.<\/jats:p>","DOI":"10.1128\/jcm.00202-09","type":"journal-article","created":{"date-parts":[[2009,8,27]],"date-time":"2009-08-27T01:45:10Z","timestamp":1251337510000},"page":"3161-3169","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["<i>emm1<\/i>\/Sequence Type 28 Strains of Group A Streptococci That Express<i>covR<\/i>at Early Stationary Phase Are Associated with Increased Growth and Earlier SpeB Secretion"],"prefix":"10.1128","volume":"47","author":[{"given":"Chuan","family":"Chiang-Ni","sequence":"first","affiliation":[{"name":"Institute of Basic Medical Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Xing","family":"Zheng","sequence":"additional","affiliation":[{"name":"Departments of Medical Laboratory Science and Biotechnology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueh-Ren","family":"Ho","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiu-Mei","family":"Wu","sequence":"additional","affiliation":[{"name":"Departments of Medical Laboratory Science and Biotechnology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woei-Jer","family":"Chuang","sequence":"additional","affiliation":[{"name":"Biochemistry"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee-Shin","family":"Lin","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-T.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Tzu Chi University, Hualien, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Chuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Pediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiunn-Jong","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Basic Medical Sciences"},{"name":"Departments of Medical Laboratory Science and Biotechnology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.1081"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-3"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.10.3311-3318.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04255.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404163101"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.17.9862"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.11.4744-4750.1996"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3523-3534.2000"},{"key":"e_1_3_2_10_2","first-page":"27","volume":"189","year":"2008","unstructured":"Chaussee, M. 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