{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:31:22Z","timestamp":1787016682118,"version":"build-2736575974"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Defining type-specific human papillomavirus (HPV) infections within cervical tissues is important for understanding the pathogenesis of cervical neoplasia and assessing the effectiveness of prophylactic vaccines with limited type-specific spectra. We compared HPV DNA-testing results from 146 matched exfoliated-cell and formalin-fixed-tissue specimens collected by cervicovaginal lavage (CVL) within 90 days of each other from women with histologically confirmed cervical intraepithelial lesions (CIN). The CVL specimens were HPV typed using a MY09\/11 L1 consensus primer PCR method followed by dot blot hybridization. The tissue specimens were HPV typed using an SPF\n                    <jats:sub>10<\/jats:sub>\n                    line probe assay HPV detection system. Of the 146 specimen pairs with evidence of CIN in the tissue, 91.8% were positive for one or more HPV types in both the tissue and cellular specimens. Tissue sections were more likely to be HPV negative (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01). Typing directly from tissue sections resolved multiple infections detected in exfoliated cells to a single HPV type in only 46.9% of cases. Combined use of both specimen types to attribute lesions to HPV type 16 (HPV-16) and\/or -18 led to 43.1% attributed to HPV-16 and\/or -18 by both specimen types and 19.9% attributed to HPV-16 and\/or -18 by one, but not both, specimen types. Unambiguous attribution of cervical lesions to a single, specific HPV type remains a difficult proposition. Use of multiple specimen types or the development of highly sensitive and robust in situ hybridization HPV-testing methods to evaluate the certainty of attribution of lesions to HPV types might provide insights in future efforts, including HPV vaccine trials.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00216-07","type":"journal-article","created":{"date-parts":[[2007,8,15]],"date-time":"2007-08-15T20:29:32Z","timestamp":1187209772000},"page":"3245-3250","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Human Papillomavirus (HPV) Genotyping Using Paired Exfoliated Cervicovaginal Cells and Paraffin-Embedded Tissues To Highlight Difficulties in Attributing HPV Types to Specific Lesions"],"prefix":"10.1128","volume":"45","author":[{"given":"Patti E.","family":"Gravitt","sequence":"first","affiliation":[{"name":"Departments of Epidemiology and Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leen Jan","family":"van Doorn","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratories, Voorburg, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"Quint","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratories, Voorburg, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Schiffman","sequence":"additional","affiliation":[{"name":"Hormonal and Reproductive Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20852-7234"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Hildesheim","sequence":"additional","affiliation":[{"name":"Hormonal and Reproductive Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20852-7234"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew G.","family":"Glass","sequence":"additional","affiliation":[{"name":"Kaiser Permanente, Portland, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda B.","family":"Rush","sequence":"additional","affiliation":[{"name":"Kaiser Permanente, Portland, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared","family":"Hellman","sequence":"additional","affiliation":[{"name":"Information Management Services, Silver Spring, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E.","family":"Sherman","sequence":"additional","affiliation":[{"name":"Hormonal and Reproductive Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20852-7234"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Burk","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine, Bronx, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia S.","family":"Wang","sequence":"additional","affiliation":[{"name":"Hormonal and Reproductive Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20852-7234"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"114","volume":"12","year":"2003","unstructured":"Alvarez, R., M. Conner, H. Weiss, P. Klug, S. Niwas, U. Manne, et al. 2003. The efficacy of 9-cis-retinoic acid (aliretinoin) as a chemopreventive agent for cervical dysplasia: results of a randomized double-blind clinical trial. Cancer Epidemiol. Biomarkers Prev.12:114-119.","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"key":"e_1_3_1_3_2","first-page":"131","year":"1992","unstructured":"Bauer, H. M., C. E. Greer, and M. M. Manos. 1992. Determination of genital human papillomavirus infection by consensus PCR amplification, p. 131-152. In C. S. Herrington and J. O. D. McGee (ed.), Diagnostic molecular pathology: a practical approach, vol. II. Oxford University Press, Oxford, United Kingdom.","journal-title":"Diagnostic molecular pathology: a practical approach"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10220"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(89)90947-2"},{"key":"e_1_3_1_6_2","first-page":"81","year":"2004","unstructured":"Gravitt, P. E., and R. Viscidi. 2004. Measurement of exposure, p. 81-100. In T. Rohan and K. V. Shah (ed.), Cervical cancer from etiology to prevention. Kluwer Academic Publishers, Dordrecht, The Netherlands.","journal-title":"Cervical cancer from etiology to prevention"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.357-361.2000"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68439-0"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.01.091"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2508-2517.1999"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65688-X"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/91.11.954"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021641"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.855"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/95.1.46"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/94.2.102"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-2599"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.979-983.2002"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(00)00244-5"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00216-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00216-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:28:25Z","timestamp":1646440105000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00216-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1128\/JCM.00216-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00216-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]},"assertion":[{"value":"2007-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}