{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:18:00Z","timestamp":1772824680512,"version":"3.50.1"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A novel rapid peptide nucleic acid fluorescence\n            <jats:italic>in situ<\/jats:italic>\n            hybridization (FISH) method,\n            <jats:named-content content-type=\"genus-species\">Staphylococcus Quick<\/jats:named-content>\n            FISH, for the direct detection of\n            <jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>\n            species from positive blood culture bottles was evaluated in a multicenter clinical study. The method utilizes a microscope slide with predeposited positive- and negative-control organisms and a self-reporting 15-min hybridization step, which eliminates the need for a wash step. Five clinical laboratories tested 722 positive blood culture bottles containing Gram-positive cocci in clusters. The sensitivities for detection of\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            and coagulase-negative staphylococci (CoNS) were 99.5% (217\/218) and 98.8% (487\/493), respectively, and the combined specificity of the assay was 89.5% (17\/19). The combined positive and negative predictive values of the assay were 99.7% (696\/698) and 70.8% (17\/24), respectively. Studies were also performed on spiked cultures to establish the specificity and performance sensitivity of the method.\n            <jats:named-content content-type=\"genus-species\">Staphylococcus Quick<\/jats:named-content>\n            FISH has a turnaround time (TAT) of &lt;30 min and a hands-on time (HOT) of &lt;5 min. The ease and speed of the method have the potential to improve the accuracy of therapeutic intervention by providing\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            \/CoNS identification simultaneously with Gram stain results.\n          <\/jats:p>","DOI":"10.1128\/jcm.00225-12","type":"journal-article","created":{"date-parts":[[2012,4,5]],"date-time":"2012-04-05T04:28:21Z","timestamp":1333600101000},"page":"1994-1998","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Multicenter Evaluation of the Staphylococcus QuickFISH Method for Simultaneous Identification of Staphylococcus aureus and Coagulase-Negative Staphylococci Directly from Blood Culture Bottles in Less than 30 Minutes"],"prefix":"10.1128","volume":"50","author":[{"given":"Melissa K.","family":"Deck","sequence":"first","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica S.","family":"Anderson","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca J.","family":"Buckner","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia","family":"Colasante","sequence":"additional","affiliation":[{"name":"Health Network Laboratories, Allentown, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Coull","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Crystal","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts, 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USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa L.","family":"Klimas","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Lindao","sequence":"additional","affiliation":[{"name":"Health Network Laboratories, Allentown, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle C.","family":"Meltzer","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margie","family":"Morgan","sequence":"additional","affiliation":[{"name":"Cedars-Sinai Medical Center, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janeen","family":"Shepard","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts, 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