{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:50:23Z","timestamp":1762865423302},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:00:00Z","timestamp":1217548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The performance of a new commercial PCR-enzyme-linked immunosorbent assay (ELISA) (Cryptodiag; Bio Advance, France) for the diagnosis of cryptosporidiosis and the identification of\n            <jats:italic>Cryptosporidium hominis<\/jats:italic>\n            and\n            <jats:italic>C. parvum<\/jats:italic>\n            from stool samples was examined. This test is based on PCR amplification of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            DNA extracted from stools, followed by an ELISA based on hybridization with\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            sp.-,\n            <jats:italic>C. hominis<\/jats:italic>\n            -, or\n            <jats:italic>C. parvum<\/jats:italic>\n            -specific probes. In spiking experiments, approximately five oocysts were detected either in water or in stool suspensions while assessing for the efficient removal of stool PCR inhibitors. No cross-reactivity was observed in the detection of\n            <jats:italic>C. parvum<\/jats:italic>\n            and\n            <jats:italic>C. homin<\/jats:italic>\n            is using the respective specific probes. Thirty-three fecal samples from patients with microscopically proven cryptosporidiosis and 118 from patients with or without other digestive protozoan infections were tested by Cryptodiag, blinded to the results of microscopy. Compared to microscopy, the sensitivity of Cryptodiag was 97.0% (32\/33) and 100% (33\/33), including the gray zone, and specificity was 98.3% (116\/118) and 96.6% (114\/118), including the gray zone. Among 34 positive results, Cryptodiag identified 19 due to\n            <jats:italic>C. hominis<\/jats:italic>\n            , 8 due to\n            <jats:italic>C. parvum<\/jats:italic>\n            , and 7 due to\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            spp. Genotyping by Cryptodiag agreed with reference typing methods in 85% of cases of\n            <jats:italic>C. parvum<\/jats:italic>\n            or\n            <jats:italic>C. hominis<\/jats:italic>\n            infections. Cryptodiag proved to be reliable and sensitive for the diagnosis of cryptosporidiosis. The use of specific probes allowed the identification of\n            <jats:italic>C. hominis<\/jats:italic>\n            and\n            <jats:italic>C. parvum<\/jats:italic>\n            , i.e., the two main species responsible for human cryptosporidiosis, and rapidly provided information on the possible source of infection.\n          <\/jats:p>","DOI":"10.1128\/jcm.00226-08","type":"journal-article","created":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T01:14:21Z","timestamp":1213319661000},"page":"2590-2594","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Assessment of Cryptodiag for Diagnosis of Cryptosporidiosis and Genotyping\n            <i>Cryptosporidium<\/i>\n            Species"],"prefix":"10.1128","volume":"46","author":[{"given":"C.","family":"Savin","sequence":"first","affiliation":[{"name":"Bio Advance, Espace Villa Parc, L'E\u0301rable, 1 avenue Marne et Gondoire, 77600 Bussy Saint Martin, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Sarfati","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Faculte\u0301 de Me\u0301decine Denis Diderot, Universite\u0301 Paris 7, 16 rue Henri Huchard, 75018 Paris, France"},{"name":"Laboratoire de Parasitologie-Mycologie, Ho\u0302pital Saint-Louis, Assistance Publique-Ho\u0302pitaux de Paris, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Menotti","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Faculte\u0301 de Me\u0301decine Denis Diderot, Universite\u0301 Paris 7, 16 rue Henri Huchard, 75018 Paris, France"},{"name":"Laboratoire de Parasitologie-Mycologie, Ho\u0302pital Saint-Louis, Assistance Publique-Ho\u0302pitaux de Paris, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Jaouhari","sequence":"additional","affiliation":[{"name":"Bio Advance, Espace Villa Parc, L'E\u0301rable, 1 avenue Marne et Gondoire, 77600 Bussy Saint Martin, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Wurtzer","sequence":"additional","affiliation":[{"name":"Bio Advance, Espace Villa Parc, L'E\u0301rable, 1 avenue Marne et Gondoire, 77600 Bussy Saint Martin, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. 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F.","family":"Garin","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Faculte\u0301 de Me\u0301decine Denis Diderot, Universite\u0301 Paris 7, 16 rue Henri Huchard, 75018 Paris, France"},{"name":"Laboratoire de Parasitologie-Mycologie, Ho\u0302pital Saint-Louis, Assistance Publique-Ho\u0302pitaux de Paris, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Derouin","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Faculte\u0301 de Me\u0301decine Denis Diderot, Universite\u0301 Paris 7, 16 rue Henri Huchard, 75018 Paris, France"},{"name":"Laboratoire de Parasitologie-Mycologie, Ho\u0302pital Saint-Louis, Assistance Publique-Ho\u0302pitaux de Paris, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2007.02873.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00138-07"},{"key":"e_1_3_2_4_2","first-page":"185","volume":"47","year":"2005","unstructured":"Caccio, S. 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