{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:09:47Z","timestamp":1790694587409,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"BioMerieux"},{"DOI":"10.13039\/100000054","name":"HHS | NIH | National Cancer Institute","doi-asserted-by":"publisher","award":["CA008748"],"award-info":[{"award-number":["CA008748"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This multicenter study was designed to assess the accuracy and reproducibility of the Vitek MS v3.0 matrix-assisted laser desorption ionization\u2013time of flight (MALDI-TOF) mass spectrometry system for identification of\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            species compared to DNA sequencing. A total of 963 clinical isolates representing 51 taxa were evaluated. In all, 663 isolates were correctly identified to the species level (69%), with another 231 (24%) correctly identified to the complex or group level. Fifty-five isolates (6%) could not be identified despite repeat testing. All of the tuberculous mycobacteria (45\/45; 100%) and most of the nontuberculous mycobacteria (569\/606; 94%) were correctly identified at least to the group or complex level. However, not all species or subspecies within the\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">M. avium<\/jats:named-content>\n            complexes and within the\n            <jats:named-content content-type=\"genus-species\">M. fortuitum<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">M. mucogenicum<\/jats:named-content>\n            groups could be differentiated. Among the 312\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            isolates tested, 236 (76%) were correctly identified to the species level, with an additional 44 (14%) correctly identified to the complex level. Species within the\n            <jats:named-content content-type=\"genus-species\">N. nova<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. transvalensis<\/jats:named-content>\n            complexes could not always be differentiated. Eleven percent of the isolates (103\/963) underwent repeat testing in order to get a final result. Identification of a representative set of\n            <jats:named-content content-type=\"genus-species\">Mycobacterium<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            species was highly reproducible, with 297 of 300 (99%) replicates correctly identified using multiple kit lots, instruments, analysts, and sites. These findings demonstrate that the system is robust and has utility for the routine identification of mycobacteria and\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            in clinical practice.\n          <\/jats:p>","DOI":"10.1128\/jcm.00237-18","type":"journal-article","created":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T14:12:19Z","timestamp":1523542339000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species"],"prefix":"10.1128","volume":"56","author":[{"given":"Barbara A.","family":"Body","sequence":"first","affiliation":[{"name":"Department of Esoteric Microbiology, Lab. Corp. of America Holdings, Burlington, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melodie A.","family":"Beard","sequence":"additional","affiliation":[{"name":"Department of Esoteric Microbiology, Lab. Corp. of America Holdings, Burlington, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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