{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:30:50Z","timestamp":1790677850285,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T00:00:00Z","timestamp":1498176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-33"],"award-info":[{"award-number":["ANR-10-LABX-33"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The global spread of carbapenemase-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flow immunoassay (LFIA) (called the NDM LFIA) for the rapid and reliable detection of NDM-like carbapenemase-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            from culture colonies. We evaluated the NDM LFIA using 175 reference enterobacterial isolates with characterized \u03b2-lactamase gene content and 74 nonduplicate consecutive carbapenem-resistant clinical isolates referred for expertise to the French National Reference Center (NRC) for Antibiotic Resistance during a 1-week period (in June 2016). The reference collection included 55 non-carbapenemase producers and 120 carbapenemase producers, including 27 NDM producers. All 27 NDM-like carbapenemase producers of the reference collection were correctly detected in less than 15 min by the NDM LFIA, including 22 strains producing NDM-1, 2 producing NDM-4, 1 producing NDM-5, 1 producing NDM-7, and 1 producing NDM-9. All non-NDM-1 producers gave a negative result with the NDM LFIA. No cross-reaction was observed with carbapenemases (VIM, IMP, NDM, KPC, and OXA-48-like), extended-spectrum \u03b2-lactamases (ESBLs) (TEM, SHV, and CTX-M), AmpCs (CMY-2, DHA-2, and ACC-1), and oxacillinases (OXA-1, -2, -9, and -10). Similarly, among the 74 referred nonduplicate consecutive clinical isolates, all 7 NDM-like producers were identified. Overall, the sensitivity and specificity of the assay were 100% for NDM-like carbapenemase detection with strains cultured on agar. The NDM LFIA was efficient, rapid, and easy to implement in the routine workflow of a clinical microbiology laboratory for the confirmation of NDM-like carbapenemase-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.00248-17","type":"journal-article","created":{"date-parts":[[2017,4,13]],"date-time":"2017-04-13T00:40:22Z","timestamp":1492044022000},"page":"2018-2029","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Development and Validation of a Lateral Flow Immunoassay for Rapid Detection of NDM-Producing Enterobacteriaceae"],"prefix":"10.1128","volume":"55","author":[{"given":"Herv\u00e9","family":"Boutal","sequence":"first","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse, CEA, INRA, Universit\u00e9 Paris-Saclay, Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Naas","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Devilliers","sequence":"additional","affiliation":[{"name":"Service de Pharmacologie et Immunoanalyse, CEA, INRA, Universit\u00e9 Paris-Saclay, Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saoussen","family":"Oueslati","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7384","authenticated-orcid":false,"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Bernabeu","sequence":"additional","affiliation":[{"name":"EA7361, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene Unit, APHP, H\u00f4pital Bic\u00eatre, Le Kremlin-Bic\u00eatre, 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