{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:40:40Z","timestamp":1791614440787,"version":"4.3.4"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Uppsala University\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            multilocus sequence type (MLST) database (\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/mlstdb.bmc.uu.se\">http:\/\/mlstdb.bmc.uu.se<\/jats:ext-link>\n            ) is based on five target regions (non-housekeeping genes) and the\n            <jats:italic>ompA<\/jats:italic>\n            gene. Each target has various numbers of alleles\u2014\n            <jats:italic>hctB<\/jats:italic>\n            , 89; CT058, 51; CT144, 30; CT172, 38; and\n            <jats:italic>pbpB<\/jats:italic>\n            , 35\u2014derived from 13 studies. Our aims were to perform an overall analysis of all\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            MLST sequence types (STs) in the database, examine STs with global spread, and evaluate the phylogenetic capability by using the five targets. A total of 415 STs were recognized from 2,089 specimens. The addition of 49\n            <jats:italic>ompA<\/jats:italic>\n            gene variants created 459 profiles. ST variation and their geographical distribution were characterized using eBURST and minimum spanning tree analyses. There were 609 samples from men having sex with men (MSM), with 4 predominating STs detected in this group, comprising 63% of MSM cases. Four other STs predominated among 1,383 heterosexual cases comprising, 31% of this group. The diversity index in ocular trachoma cases was significantly lower than in sexually transmitted chlamydia infections. Predominating STs were identified in 12 available\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            whole genomes which were compared to 22\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            full genomes without predominating STs. No specific gene in the 12 genomes with predominating STs could be linked to successful spread of certain STs. Phylogenetic analysis showed that MLST targets provide a tree similar to trees based on whole-genome analysis. The presented MLST scheme identified\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            strains with global spread. It provides a tool for epidemiological investigations and is useful for phylogenetic analyses.\n          <\/jats:p>","DOI":"10.1128\/jcm.00249-15","type":"journal-article","created":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T02:21:46Z","timestamp":1430360506000},"page":"2172-2179","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Global Multilocus Sequence Type Analysis of Chlamydia trachomatis Strains from 16 Countries"],"prefix":"10.1128","volume":"53","author":[{"given":"Bj\u00f6rn","family":"Herrmann","sequence":"first","affiliation":[{"name":"Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Isaksson","sequence":"additional","affiliation":[{"name":"Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Ryberg","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanette","family":"T\u00e5ngrot","sequence":"additional","affiliation":[{"name":"Bioinformatics Infrastructure for Life Sciences (BILS) and Computational Life Science Cluster (CLiC), Department of Molecular Biology, Ume\u00e5 University, Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isam","family":"Saleh","sequence":"additional","affiliation":[{"name":"Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Versteeg","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Public Health Service of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Gravningen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control, University Hospital of North Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Bruisten","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Public Health Service of Amsterdam, Amsterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Global incidence and prevalence of selected curable sexually transmitted infections\u20142008","author":"World Health Organization","year":"2012","unstructured":"World Health Organization. 2012. 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