{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:15:09Z","timestamp":1790644509294,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Disseminated toxoplasmosis is a life-threatening infection in transplant recipients, which results either from reactivation of latent infection or from organ-transmitted primary infection. Preventive measures and diagnostic screening methods differ between countries and are related to the seroprevalence of<jats:italic>Toxoplasma<\/jats:italic>spp. in the general population. Here we report a case of disseminated toxoplasmosis in a heart transplant recipient with previous immunity that occurred after cotrimoxazole prophylaxis for the prevention of<jats:italic>Pneumocystis jiroveci<\/jats:italic><jats:italic>i<\/jats:italic>pneumonia was stopped. Quantitative PCR proved useful for the diagnosis and monitoring of<jats:italic>Toxoplasma<\/jats:italic>infection. Decreasing parasitic burdens in sequential samples of cerebrospinal fluid, blood, and bronchoalveolar lavage fluid correlated with a favorable outcome and allowed modulation of the immunosuppressive drug regimen. The duration of anti-<jats:italic>Toxoplasma<\/jats:italic>treatment and the need for maintenance prophylaxis are discussed, as well as prophylaxis for solid-organ transplant recipients. Although a rare event in heart transplant recipients,<jats:italic>Toxoplasma<\/jats:italic>reactivation must be investigated promptly, since early treatment improves the prognosis.<\/jats:p>","DOI":"10.1128\/jcm.00252-10","type":"journal-article","created":{"date-parts":[[2010,5,13]],"date-time":"2010-05-13T02:15:16Z","timestamp":1273716916000},"page":"2541-2545","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Correlation of Parasite Load Determined by Quantitative PCR to Clinical Outcome in a Heart Transplant Patient with Disseminated Toxoplasmosis"],"prefix":"10.1128","volume":"48","author":[{"given":"Sole\u0300ne","family":"Patrat-Delon","sequence":"first","affiliation":[{"name":"Service de Re\u0301animation Me\u0301dicale et Maladies Infectieuses"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Gangneux","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Lavoue\u0301","sequence":"additional","affiliation":[{"name":"Service de Re\u0301animation Me\u0301dicale et Maladies Infectieuses"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Lelong","sequence":"additional","affiliation":[{"name":"Service de Cardiologie, Centre Hospitalier et Universitaire de Rennes, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Guiguen","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"le Tulzo","sequence":"additional","affiliation":[{"name":"Service de Re\u0301animation Me\u0301dicale et Maladies Infectieuses"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Robert-Gangneux","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/597477"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000044864.99398.F1"},{"key":"e_1_3_2_4_2","first-page":"1380","volume":"25","year":"2006","unstructured":"Baran, D. A., M. M. Alwarshetty, S. Alvi, L. H. Arroyo, S. Lubitz, S. Pinney, A. L. Gass, and M. J. Zucker. 2006. Is toxoplasmosis prophylaxis necessary in cardiac transplantation? Long-term follow-up at two transplant centers. J. Heart Lung Transplant. 25 : 1380-1382.","journal-title":"Long-term follow-up at two transplant centers. J. 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Prophylaxis of toxoplasmosis infection with pyrimethamine\/sulfadoxine (Fansidar) in bone marrow transplant recipients. Bone Marrow Transplant. 14 : 241-245.","journal-title":"Bone Marrow Transplant."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2005.00086.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/595709"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(96)80059-2"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01690095"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.20537"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e318169bebd"},{"key":"e_1_3_2_20_2","first-page":"27","volume":"99","year":"1983","unstructured":"Luft, B. J., Y. Naot, F. G. Araujo, E. B. Stinson, and J. S. Remington. 1983. Primary and reactivated Toxoplasma infection in patients with cardiac transplants. Clinical spectrum and problems in diagnosis in a defined population. Ann. Intern. Med. 99 : 27-31.","journal-title":"Clinical spectrum and problems in diagnosis in a defined population. Ann. Intern. 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