{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:14:33Z","timestamp":1760645673090},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Molecular methods such as PCR have become attractive tools for diagnosis of cutaneous leishmaniasis (CL), both for their high sensitivity and for their specificity. However, their practical use in routine diagnosis is limited due to the infrastructural requirements and the lack of any standardization. Recently, a simplified and standardized PCR format for molecular detection of\n            <jats:italic>Leishmania<\/jats:italic>\n            was developed. The\n            <jats:italic>Leishmania<\/jats:italic>\n            OligoC-TesT is based on simple and rapid detection using a dipstick with PCR-amplified\n            <jats:italic>Leishmania<\/jats:italic>\n            DNA. In this study, we estimated the diagnostic accuracy of the\n            <jats:italic>Leishmania<\/jats:italic>\n            OligoC-TesT for 61 specimens from 44 CL-suspected patients presenting at the leishmaniasis clinic of the Instituto de Medicina Tropical Alexander von Humboldt, Peru. On the basis of parasitological detection and the leishmanin skin test (LST), patients were classified as (i) confirmed CL cases, (ii) LST-positive cases, and (iii) LST-negative cases. The sensitivities of the\n            <jats:italic>Leishmania<\/jats:italic>\n            OligoC-TesT was 74% (95% confidence interval (CI), 60.5% to 84.1%) for lesion aspirates and 92% (95% CI, 81.2% to 96.9%) for scrapings. A significantly higher sensitivity was observed with a conventional PCR targeting the kinetoplast DNA on the aspirates (94%) (\n            <jats:italic>P<\/jats:italic>\n            = 0.001), while there was no significant difference in sensitivity for the lesion scrapings (88%) (\n            <jats:italic>P<\/jats:italic>\n            = 0.317). In addition, the\n            <jats:italic>Leishmania<\/jats:italic>\n            OligoC-TesT was evaluated for 13 CL-suspected patients in two different peripheral health centers in the central jungle of Peru. Our findings clearly indicate the high accuracy of the\n            <jats:italic>Leishmania<\/jats:italic>\n            OligoC-TesT for lesion scrapings for simple and rapid molecular diagnosis of CL in Peru.\n          <\/jats:p>","DOI":"10.1128\/jcm.00259-09","type":"journal-article","created":{"date-parts":[[2009,6,25]],"date-time":"2009-06-25T02:13:44Z","timestamp":1245896024000},"page":"2560-2563","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["<i>Leishmania<\/i>\n            OligoC-TesT as a Simple, Rapid, and Standardized Tool for Molecular Diagnosis of Cutaneous Leishmaniasis in Peru"],"prefix":"10.1128","volume":"47","author":[{"given":"Diego","family":"Espinosa","sequence":"first","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea K.","family":"Boggild","sequence":"additional","affiliation":[{"name":"Tropical Diseases Unit, Division of Infectious Diseases, University Health Network, Toronto General Hospital, Toronto, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stijn","family":"Deborggraeve","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Laurent","sequence":"additional","affiliation":[{"name":"Coris BioConcept, Gembloux, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian","family":"Valencia","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Universidad Nacional San Antonio Abad del Cusco, Cusco, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ce\u0301sar","family":"Miranda-Vera\u0301stegui","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Llanos-Cuentas","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Leclipteux","sequence":"additional","affiliation":[{"name":"Coris BioConcept, Gembloux, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Claude","family":"Dujardin","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Bu\u0308scher","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Are\u0301valo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru"},{"name":"Departamento de Bioquimica, Biologia Molecular y Farmacologia, Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/518041"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1435-1439.2006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01286-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762004000700001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1983.32.475"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02594-05"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/592509"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/375273"},{"key":"e_1_3_2_10_2","first-page":"692","volume":"2","year":"2004","unstructured":"Desjeux, P. 2004. 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